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Research
(Published
online: 06-09-2014)
4.
Detection of natural prevalence and
infection of ixodid ticks with Theileria equi in hilly
equines of Palam valley (India) -
Prateek Kashyap, Aman
D. Moudgil and Pallavi
Veterinary World, 7(9): 652-655
doi:
10.14202/vetworld.2014.652-655
Prateek Kashyap: ERA and Brooke
India, Equine Welfare Unit, Palampur, Kangra, Himachal Pradesh,
India; prateekpandit0871@gmail.com
Aman D. Moudgil: Department of
Veterinary Parasitology, College of Veterinary Science, Guru Angad
Dev Veterinary & Animal Sciences University, Ludhiana, Punjab,
India; moudgil.aman@gmail.com
Pallavi: Division of Veterinary Public
Health, Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar Pradesh, India; upadhyayapallavi.31@gmail.com
Received: 01-06-2014, Revised: 22-07-2014,
Accepted: 31-07-2014. Published Online: 06-09-2014
Corresponding author: Prateek
Kashyap, email: prateekpandit0871@gmail.com
Abstract
Aim:
The aim was to study the prevalence of tick infestation in
equines of Palam valley and specific detection of Theileria
equi infection in tick samples with nested polymerase chain
reaction (PCR) based assay.
Materials and Methods: In order to study the prevalence of
ixodid tick population in hilly equines and their potential role
in the transmission of T. equi, a total of 74 ticks were
collected from apparently healthy equines, which were then
processed and identified by classical parasitological technique.
The molecular techniques (nested PCR) were applied for
identification of infection of T. equi.
Results: The ticks (n=74) collected from apparently healthy
equines belonged mainly to three different species, of which 42
(56.75%) were Rhipicephalus microplus, whereas 16 (21.62%)
were of Hyalomma species and 16 (21.62%) were of
Hemaphysalis species. A total of 21 (30%) ticks were recovered
from male and 53 (75.7%) from female equines. Adult equines
harbored 94.6% (n=70) when compared to 5.4% (n=4) harbored by
young ones. On nested PCR amplification an amplicon of 665 bp size
specific for T. equi was detected in 6.75% (5/74) ticks (in
7.5% ticks recovered from a female and 4.7% from male equines).
Conclusion: Nested PCR assay resulted in significantly higher
efficacy of detection of the parasite in ticks. These results
clearly demonstrate the presence of equine theileriosis in hilly
northern state of the country and potential roles of ticks (R.
microplus, Haemaphysalis and Hyalomma species)
in its transmission.
Keywords: equines, Himachal Pradesh, nested
polymerase chain reaction, Theileria equi
References
1. Kumar, S., Kumar, R. and Sugimoto, C. (2009) A perspective
on Theileria equi in donkeys. Jpn. J. Vet. Res., 56(4):
171-180.
PMid:19358444 |
|
|
2. Rothschild, C.M. and Knowles, D.P. (2007) Equine
piroplasmosis. In: Equine Infectious Diseases. Saunders
Elsevier, St. Louis, MO. p465-473.
http://dx.doi.org/10.1016/B978-1-4160-2406-4.50065-X |
|
|
3. De Waal, D.T. (1992) Equine piroplasmosis: A review. Br.
Vet. J., 148: 6-14.
http://dx.doi.org/10.1016/0007-1935(92)90061-5 |
|
|
4. Abedi, B., Razmi, G., Seifi, H. and Naghibi, A. (2014)
Molecular and serological detection of Theileria equi and
Babesia caballi infection in horses and ixodid ticks in Iran.
Ticks Tick Borne Dis., 5(3): 239-244.
http://dx.doi.org/10.1016/j.ttbdis.2013.11.008
PMid:24556274 |
|
|
|
5. Takeet, M., Adeleye, A., Adebayo, O.O. and Akande, F.
(2009) Haematology and serum biochemical alteration in stress
induced equine theileriosis. A case report. Sci. World J., 4:
19-21. |
|
|
6. Jaffer, O., Abdishakur, F., Hakimuddin, F., Riya, A.,
Wernery, U. and Schuster, R.K. (2010) A comparative study of
serological tests and PCR for the diagnosis of equine
piroplasmosis. Parasitol. Res., 106(3): 709-713.
http://dx.doi.org/10.1007/s00436-009-1669-5
PMid:19894063 |
|
|
7. Mujica, F.F., Perrone, T., Forlano, M., Coronado, A.,
Melendez, R.D., Barrios, N., Alvarez, R., and Granda, F.
(2011) Serological prevalence of Babesia caballi and Theileria
equi in horses of Lara State, Venezuela. Vet. Parasitol.,
178(1-2): 180-183.
http://dx.doi.org/10.1016/j.vetpar.2010.12.036
PMid:21273001 |
|
|
8. Abutarbush, S.M., Alqawasmeh, D.M., Mukbel, R.M. and Al-Majali,
A.M. (2012) Equine babesiosis: Seroprevalence, risk factors
and comparison of different diagnostic methods in Jordan.
Transbound Emerg. Dis., 59(1): 72-78.
http://dx.doi.org/10.1111/j.1865-1682.2011.01244.x
PMid:21771287 |
|
|
|
9. García-Bocanegra, I., Arenas-Montes, A., Hernández, E.,
Adaszek, L., Carbonero, A., Almería, S., Jaén-Téllez, J.A.,
Gutiérrez-Palomino, P., and Arenas, A. (2013) Seroprevalence
and risk factors associated with Babesia caballi and Theileria
equi infection in equids. Vet. J., 19(2)5: 172-178. |
|
|
10. Farkas, R., Tánczos, B., Gyurkovszky, M., Földvári, G.,
Solymosi, N., Edelhofer, R. and Hornok, S. (2013) Serological
and molecular detection of Theileria equi infection in horses
in Hungary. Vet. Parasitol., 192(1-3): 143-148.
http://dx.doi.org/10.1016/j.vetpar.2012.09.035
PMid:23099089 |
|
|
11. Bose, R., Jorgensen, W.K., Dalgliesh, R.J., Friedhoff, K.T.
and De Vos, A.J. (1995) Current state and future trends in the
diagnosis of babesiosis. Vet. Parasitol., 57(1-3): 61-74.
http://dx.doi.org/10.1016/0304-4017(94)03111-9 |
|
|
12. Bahiruddin, J.B., Camma, C. and Rebelo, E. (1999)
Molecular detection of Babesia equi and Babesia caballi in
horse blood by PCR amplification of part of the 16S rRNA gene.
Vet. Parasitol., 84(1-2): 75-83.
http://dx.doi.org/10.1016/S0304-4017(99)00049-7 |
|
|
13. Nicolaiewsky, T.B., Richter, M.F., Lunge, V.R., Cunha, C.W.,
Delagostin, O., Ikuta, N., Fonseca, A.S., Silva, S.S. and
Ozaki, L.S. (2001) Detection of Babesia equi (Laveran, 1901)
by nested polymerase chain reaction. Vet. Parasitol., 101(1):
9-21.
http://dx.doi.org/10.1016/S0304-4017(01)00471-X |
|
|
14. Battsetseg, B., Lucero, S., Xuan, X., Claveria, F.G.,
Inoue, N., Alhassan, A., Kanno, T., Igarashi, I., Nagasawa,
H., Mikami, T. and Fujisaki, K. (2002) Detection of natural
infection of Boophilus microplus with Babesia equi and Babesia
caballi in Brazilian horses using nested polymerase chain
reaction. Vet. Parasitol., 22: 351-357.
http://dx.doi.org/10.1016/S0304-4017(02)00131-0 |
|
|
15. Rampersad, J., Cesar, E., Campell, M.D., Samlal, M. and
Ammons, D. (2003) A field eval-uation of PCR for the routine
detection of Babesia equi in horses. Vet. Parasitol., 114(2):
81-87.
http://dx.doi.org/10.1016/S0304-4017(03)00129-8 |
|
|
|
16. Hoogstraal, H. (1956) In: African Ixodoidea. Ticks of the
Sudan, Vol. 1. US Naval Medical Research Unit No. 3, Cairo,
Egypt. |
|
|
17. Estrada-Pena, A., Bouattour, A., Camicas, J.L. and Walker,
A.R. (2004) In: Ticks of Domestic Animals in the Mediterranean
Region, a Guide to Identification of Species. Bioscience
Reports, UK.
PMCid:PMC3320401 |
|
|
|
18. Al-Saad, K.M. (2009) Acute babesiosis in foals. J. Anim.
Vet. Adv., 8: 2585-2589. |
|
|
19. Baldani, C.D., Nakaghi, A.C., Machado, R.Z. (2010)
Occurrence of Theileria equi in horses raised in the
Jaboticabal microregion, Săo Paulo State, Brazil. Rev. Bras.
Parasitol. Vet., 19: 228-232.
http://dx.doi.org/10.1590/S1984-29612010000400007
PMid:21184699 |
|
|
20. Lori, A., Gabrielli, S., Calderini, P., Moretti, A.,
Pietrobelli, M., Tampieri, M.P., Galuppi, R. and Cancrini, G.
(2010) Tick reservoirs for piroplasms in central and Northern
Italy. Vet. Parasitol., 170(3-4): 291-296.
http://dx.doi.org/10.1016/j.vetpar.2010.02.027
PMid:20304560 |
|
|
21. Moretti, A., Mangili, V., Salvatori, R., Maresca, C.,
Scoccia, E., Torina, A., Moretta, I., Gabrielli, S., Tampieri,
M.P. and Pietrobelli, M. (2010) Prevalence and diagnosis of
Babesia and Theileria infections in horses in Italy: A
preliminary study. Vet. J., 184(3): 346-350.
http://dx.doi.org/10.1016/j.tvjl.2009.03.021
PMid:19394253 |
|
|
22. Alanazi, A.D., Alyousif, M.S. and Hassieb, M.M. (2012)
Seroprevalence study on Theileria equi and Babesia caballi
antibodies in horses from central province of Saudi Arabia. J.
Parasitol., 98(5): 1015-1017.
http://dx.doi.org/10.1645/GE-2997.1
PMid:22489955 |
|
|
23. Steinman, A., Zimmerman, T., Klement, E., Lensky, I.M.,
Berlin, D., Gottlieb, Y. and Baneth, G. (2012) Demographic and
environmental risk factors for infection by Theileria equi in
590 horses in Israel. Vet. Parasitol., 187(3-4): 558-562.
http://dx.doi.org/10.1016/j.vetpar.2012.01.018
PMid:22293151 |
|
|
24. Hailat, N.Q., Lafi, S.Q., Al-Darraji, A.M. and Al-Ani, F.K.
(1997) Equine babesiosis associated with strenuous exercise:
Clinical and pathological studies in Jordan. Vet. Parasitol.,
69(1-2): 1-8.
http://dx.doi.org/10.1016/S0304-4017(96)01100-4 |
|
|
25. Scoles, G.A., Hutcheson, H.J., Schlater, J.L., Hennager,
S.G. and Knowles, D.P. (2011) Equine piroplasmosis associated
with Amblyomma cajennense ticks, Texas, USA. Emerg. Infect.
Dis., 17(10): 1903-1905.
http://dx.doi.org/10.3201/eid1710.101182
PMid:22000367 PMCid:PMC3310643 |
|
