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Research (Published online: 07-09-2014)

8. Comparison of blood profiles between healthy and Brucella affected cattle - Rita Nath, Sutopa Das, Satya Sarma and Maitrayee Devi

Veterinary World, 7(9): 668-670

 

 

   doi: 10.14202/vetworld.2014.668-670

 

 

Rita Nath: Department of Veterinary Biochemistry, College of Veterinary Science, Khanapara, Guwahati, Assam, India; ritasankr@rediffmail.com

Sutopa Das: Department of Microbiology, College of Veterinary Science, Khanapara, Guwahati, Assam, India; d_sutopa@yahoo.com

Satya Sarma: Department of Veterinary Biochemistry, College of Veterinary Science, Khanapara, Guwahati, Assam, India; sarmasatya49@yahoo.in

Maitrayee Devi: Department of Microbiology, College of Veterinary Science, Khanapara, Guwahati, Assam, India; maitrayeee@gmail.com

Received: 23-05-2014, Revised: 20-07-2014, Accepted: 27-07-2014, Published online: 07-09-2014

Corresponding author: Rita Nath, email: ritasankr@rediffmail.com



Aim: The present investigation was undertaken to evaluate the blood biochemical metabolites of crossbred cattle suffering from brucellosis, in Khetri region of Kamrup District of Assam, India.

Materials and Methods: Blood was collected by venipuncture from the jugular vein using 18 gauge hypodermic needle fitted to a Vacutainer-Leur adapter and evacuated tubes. Samples were screened for Brucella by rose Bengal plate test using plate test antigen. The study consisted of two groups. Group I consisted of healthy crossbred cows and Group II consisted of crossbred cows affected with brucellosis. Blood metabolites were estimated by spectrophotometric methods.

Results: Out of 14 serum samples, 8 samples were positive for Brucella and remaining 6 were found to be negative. It was observed that serum albumin and urea of the affected cattle showed a significant decrease (p<0.05), but serum globulin, cholesterol, triglyceride, aspartate transaminase, and alanine transaminase activities were significantly increased (p<0.05) in comparison to the healthy cattle. Glucose, protein, albumin: globulin ratio, blood urea nitrogen did not show much variation between the Brucellosis affected and healthy cattle.

Conclusion: Results obtained showed that brucellosis have a harmful effect on the animals health as they disturb the vital organs functions, which appear in the form of changes in blood biochemical parameters.

Keywords: biochemical metabolites, brucellosis, cattle, rose Bengal plate test.



1. Rajashekara, G., Eskra, L., Mathison, A., Petersen, G., Yu, Q., Harms, J. and Splitter, G. (2006) Brucella; functional genomics and host-pathogen interaction. Anim. Health. Res., 7(1-2): 1-11.
 
2. Maadi, H., Moharamnejad, M. and Haghi, M. (2011) Prevalence of brucellosis in cattle in Urmia, Iran. Pak. Vet. J., 31: 81-82.
 
3. Akhtar, R., He, Y.O, Larson, C.B., Chaudhary, Z.I. and Ahmad, M.U.D. (2012) Differential stimulatory activities of smooth and rough Brucella abortus lipopolysaccharide in murine macrophages. Pak. Vet. J., 32: 339-344.
 
4. Behzadi, M.A. and Mogheiseh, A (2011). Epidemiological survey of Brucella canis infection in different breeds of dogs in Fars province. Pak. Vet. J., 32: 234-236.
 
5. Abubakar, M., Mansoor, M. and Arshed, M.J. (2012) Bovine brucellosis: Old and new concepts with Pakistan perspective. Pak. Vet. J., 32: 147-155.
 
6. El-Boshy, M., Abbas, M., El-Khoderyl, H. and Osman, S. (2009) Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius). Vet. Med., 54(1): 25-32.
 
7. Bouhroum, N.L, Bensahli, B. and Niar, A. (2012) Evolution of biochemical parameters in post partum cows affected with Brucella, in the wilaya of Relizane. Afr. J. Biotechnol., 11(7): 1818-1822.
http://dx.doi.org/10.5897/AJB11.1461
 
8. Radostits, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D. (2007) Veterinary Medicine: A Textbook of the Diseases of Cattle, Horse, Sheep, Pigs & Goat. Saunders, St Louis. p1518-1522.
 
9. Al-Majali, A.M. (2005) Seroepidemiology of caprine Brucellosis in Jordan. Small Rumin. Res., 58: 13-18.
http://dx.doi.org/10.1016/j.smallrumres.2004.07.013
 
10. Aldomy, F., Alkhawaldeh, M. and Younis, I.B. (2009) Immune responses of goats (Shami breed) to vaccination with a full, reduced and conjunctival dose of brucevac (Brucella melitensis Rev.1) vaccine. Pak. Vet. J., 29: 149-153.
 
11. Nath, R (2008) Changes in serum enzymes during experimental aflatoxicosis in broiler chicken. Indian. Vet. J., 85(1): 10-12.
 
12. Snedecor, G.M., and Cochran, W.G. (1994). Statistical Method. 7th ed. Oxford and IBH Publishing Co, New Delhi.
 
13. Renukaradhya, G.J., Isloor, S. and Rajasekhar, M. (2002). Epidemiology, zoonotic aspects, vaccination and control/eradication of brusellosis in India. Vet. Microbiol., 90(1-4): 183-195.
http://dx.doi.org/10.1016/S0378-1135(02)00253-5
 
14. Abenga, J.N. and Anosa, V.O. (2005) Serum total protein & creatine levels in experimental Gambian trypanosomosis of Vervet monkeys. Afr. J. Biotechnol., 4: 187-190.
 
15. Hamada, D.M., Mohamed, A.H, Mabrouk, A., Emad, M. and Ah, M.E. (2013) Seroprevalence of abortion causing agents in Egyptian sheep and goat breeds and their effects on the animal's performance. J. Agric. Sci., 5(9): 92-101.
 
16. Bruce, W.J., Tajie, H.H., Nilofer, Q. and Gary, A. (2002). Splitter rough lipopolysaccharide from Brucella abortus and escherichia coli. differentially activates the same mitogen-activated protein kinase signaling pathways for tumor necrosis factor alpha in raw 264.7 macrophage-like cells. Infect. Immun., 70(12): 7165-7168.
http://dx.doi.org/10.1128/IAI.70.12.7165-7168.2002
PMCid:PMC132973
 
17. Huang, L.Y., Alibert, J., Leifer, C.A., Segal, D.M., Sher, A., Golenbock, D.T. and Golding, B. (2003) Heat-killed Brucella abortus induces TNF and IL12p40 by distinct My D88-dependent patways: TNF, unlike IL-12p40 secretion, is Toll-like receptor 2 dependent. J. Immunol., 171(3): 1441-1446.
http://dx.doi.org/10.4049/jimmunol.171.3.1441
PMid:12874236
 
18. Feingold, K.R., Soued, M., Staprand, I., Gavin, L.A., Donahue, M.E., Huang, B.J., Moser, A.H., Gulli, R. and Grumfeld, C. (1989) Effect of TNF on lipid metabolism in the diabetic rat evidence that inhibition of adipose tissue lipoprotein lipase activity is not required for TNF induced hyperlipidemia. J. Clin. Invest., 83(4): 1116-1121.
http://dx.doi.org/10.1172/JCI113991
PMid:2703526 PMCid:PMC303797
 
19. Al-Kaysi, A.M., Eid, R.A.A., and Fahmy, B.G.A. (2010) Biochemical studies on the effect of Toxoplasma infection on liver and kidney functions in mice. Egypt. J. Comp. Path. Clin. Pathol., 23: 174-185.
 
20. Al-Hussary, N.A.J. and Al-Zuhairy, A.S.M. (2010) Effect of toxoplasmosis ans brucellosis on some biochemical parameters in ewes. Iraqi. J. Vet. Sci. 24: 73-80.
 
21. Gul, S.T., Khan, A., Ahmad, M. and Hussain, I. (2013) Seroprevalence of brucellosis and associated hemato-biochemical changes in Pakistani horses. Pak. J. Agric. Sci., 50(4): 745-750.