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              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
 
 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
(Published 
online: 07-09-2014) 
              
              8. 
              Comparison of blood profiles between healthy 
              and Brucella affected cattle - 
              Rita Nath, Sutopa Das, 
              Satya Sarma and Maitrayee Devi 
              
              Veterinary World, 7(9): 668-670   
              
   
                
                
doi: 
              10.14202/vetworld.2014.668-670 
                  Rita 
              Nath: Department 
              of Veterinary Biochemistry, College of Veterinary Science, 
              Khanapara, Guwahati, Assam, India; ritasankr@rediffmail.com Sutopa 
              Das: Department of 
              Microbiology, College of Veterinary Science, Khanapara, Guwahati, 
              Assam, India; d_sutopa@yahoo.com Satya 
              Sarma: Department 
              of Veterinary Biochemistry, College of Veterinary Science, 
              Khanapara, Guwahati, Assam, India; sarmasatya49@yahoo.in 
              Maitrayee Devi: Department of Microbiology, College of 
              Veterinary Science, Khanapara, Guwahati, Assam, India;
              maitrayeee@gmail.com Received: 23-05-2014, Revised: 20-07-2014, 
              Accepted: 27-07-2014, Published online: 07-09-2014 
              
              
              Corresponding author: Rita Nath, 
              email: ritasankr@rediffmail.com 
 
              Abstract 
 Aim:
              The present investigation was undertaken to evaluate the blood 
              biochemical metabolites of crossbred cattle suffering from 
              brucellosis, in Khetri region of Kamrup District of Assam, India.
               
              Materials and Methods: Blood was collected by venipuncture 
              from the jugular vein using 18 gauge hypodermic needle fitted to a 
              Vacutainer-Leur adapter and evacuated tubes. Samples were screened 
              for Brucella by rose Bengal plate test using plate test 
              antigen. The study consisted of two groups. Group I consisted of 
              healthy crossbred cows and Group II consisted of crossbred cows 
              affected with brucellosis. Blood metabolites were estimated by 
              spectrophotometric methods. 
              Results: Out of 14 serum samples, 8 samples were positive for
              Brucella and remaining 6 were found to be negative. It was 
              observed that serum albumin and urea of the affected cattle showed 
              a significant decrease (p<0.05), but serum globulin, cholesterol, 
              triglyceride, aspartate transaminase, and alanine transaminase 
              activities were significantly increased (p<0.05) in comparison to 
              the healthy cattle. Glucose, protein, albumin: globulin ratio, 
              blood urea nitrogen did not show much variation between the 
              Brucellosis affected and healthy cattle.  
              Conclusion: Results obtained showed that brucellosis have a 
              harmful effect on the animals health as they disturb the vital 
              organs functions, which appear in the form of changes in blood 
              biochemical parameters.  
              Keywords: biochemical metabolites, 
              brucellosis, cattle, rose Bengal plate test. 
 
              References 
 
                
                  | 1. Rajashekara, G., Eskra, L., Mathison, A., Petersen, G., Yu, 
                  Q., Harms, J. and Splitter, G. (2006) Brucella; functional 
                  genomics and host-pathogen interaction. Anim. Health. Res., 
                  7(1-2): 1-11. |  
                  |  |  
                  | 2. Maadi, H., Moharamnejad, M. and Haghi, M. (2011) Prevalence 
                  of brucellosis in cattle in Urmia, Iran. Pak. Vet. J., 31: 
                  81-82. |  
                  |  |  
                  | 3. Akhtar, R., He, Y.O, Larson, C.B., Chaudhary, Z.I. and 
                  Ahmad, M.U.D. (2012) Differential stimulatory activities of 
                  smooth and rough Brucella abortus lipopolysaccharide in murine 
                  macrophages. Pak. Vet. J., 32: 339-344. |  
                  |  |  
                  | 4. Behzadi, M.A. and Mogheiseh, A (2011). Epidemiological 
                  survey of Brucella canis infection in different breeds of dogs 
                  in Fars province. Pak. Vet. J., 32: 234-236. |  
                  |  |  
                  | 5. Abubakar, M., Mansoor, M. and Arshed, M.J. (2012) Bovine 
                  brucellosis: Old and new concepts with Pakistan perspective. 
                  Pak. Vet. J., 32: 147-155. |  
                  |  |  
                  | 6. El-Boshy, M., Abbas, M., El-Khoderyl, H. and Osman, S. 
                  (2009) Cytokine response and clinicopathological findings in 
                  Brucella infected camels (Camelus dromedarius). Vet. Med., 
                  54(1): 25-32. |  
                  |  |  
                  | 7. Bouhroum, N.L, Bensahli, B. and Niar, A. (2012) Evolution 
                  of biochemical parameters in post partum cows affected with 
                  Brucella, in the wilaya of Relizane. Afr. J. Biotechnol., 
                  11(7): 1818-1822. http://dx.doi.org/10.5897/AJB11.1461
 |  
                  |  |  
                  | 8. Radostits, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, 
                  P.D. (2007) Veterinary Medicine: A Textbook of the Diseases of 
                  Cattle, Horse, Sheep, Pigs & Goat. Saunders, St Louis. 
                  p1518-1522. |  
                  |  |  
                  | 9. Al-Majali, A.M. (2005) Seroepidemiology of caprine 
                  Brucellosis in Jordan. Small Rumin. Res., 58: 13-18. http://dx.doi.org/10.1016/j.smallrumres.2004.07.013
 |  
                  |  |  
                  | 10. Aldomy, F., Alkhawaldeh, M. and Younis, I.B. (2009) Immune 
                  responses of goats (Shami breed) to vaccination with a full, 
                  reduced and conjunctival dose of brucevac (Brucella melitensis 
                  Rev.1) vaccine. Pak. Vet. J., 29: 149-153. |  
                  |  |  
                  | 11. Nath, R (2008) Changes in serum enzymes during 
                  experimental aflatoxicosis in broiler chicken. Indian. Vet. 
                  J., 85(1): 10-12. |  
                  |  |  
                  | 12. Snedecor, G.M., and Cochran, W.G. (1994). Statistical 
                  Method. 7th ed. Oxford and IBH Publishing Co, New Delhi. |  
                  |  |  
                  | 13. Renukaradhya, G.J., Isloor, S. and Rajasekhar, M. (2002). 
                  Epidemiology, zoonotic aspects, vaccination and 
                  control/eradication of brusellosis in India. Vet. Microbiol., 
                  90(1-4): 183-195. http://dx.doi.org/10.1016/S0378-1135(02)00253-5
 |  
                  |  |  
                  | 14. Abenga, J.N. and Anosa, V.O. (2005) Serum total protein & 
                  creatine levels in experimental Gambian trypanosomosis of 
                  Vervet monkeys. Afr. J. Biotechnol., 4: 187-190. |  
                  |  |  
                  | 15. Hamada, D.M., Mohamed, A.H, Mabrouk, A., Emad, M. and Ah, 
                  M.E. (2013) Seroprevalence of abortion causing agents in 
                  Egyptian sheep and goat breeds and their effects on the 
                  animal's performance. J. Agric. Sci., 5(9): 92-101. |  
                  |  |  
                  | 16. Bruce, W.J., Tajie, H.H., Nilofer, Q. and Gary, A. (2002). 
                  Splitter rough lipopolysaccharide from Brucella abortus and 
                  escherichia coli. differentially activates the same mitogen-activated 
                  protein kinase signaling pathways for tumor necrosis factor 
                  alpha in raw 264.7 macrophage-like cells. Infect. Immun., 
                  70(12): 7165-7168. http://dx.doi.org/10.1128/IAI.70.12.7165-7168.2002
 PMCid:PMC132973
 |  
                  |  |  
                  | 17. Huang, L.Y., Alibert, J., Leifer, C.A., Segal, D.M., Sher, 
                  A., Golenbock, D.T. and Golding, B. (2003) Heat-killed 
                  Brucella abortus induces TNF and IL12p40 by distinct My 
                  D88-dependent patways: TNF, unlike IL-12p40 secretion, is 
                  Toll-like receptor 2 dependent. J. Immunol., 171(3): 
                  1441-1446. http://dx.doi.org/10.4049/jimmunol.171.3.1441
 PMid:12874236
 |  
                  |  |  
                  | 18. Feingold, K.R., Soued, M., Staprand, I., Gavin, L.A., 
                  Donahue, M.E., Huang, B.J., Moser, A.H., Gulli, R. and 
                  Grumfeld, C. (1989) Effect of TNF on lipid metabolism in the 
                  diabetic rat evidence that inhibition of adipose tissue 
                  lipoprotein lipase activity is not required for TNF induced 
                  hyperlipidemia. J. Clin. Invest., 83(4): 1116-1121. http://dx.doi.org/10.1172/JCI113991
 PMid:2703526 PMCid:PMC303797
 |  
                  |  |  
                  | 19. Al-Kaysi, A.M., Eid, R.A.A., and Fahmy, B.G.A. (2010) 
                  Biochemical studies on the effect of Toxoplasma infection on 
                  liver and kidney functions in mice. Egypt. J. Comp. Path. Clin. 
                  Pathol., 23: 174-185. |  
                  |  |  
                  | 20. Al-Hussary, N.A.J. and Al-Zuhairy, A.S.M. (2010) Effect of 
                  toxoplasmosis ans brucellosis on some biochemical parameters 
                  in ewes. Iraqi. J. Vet. Sci. 24: 73-80. |  
                  |  |  
                  | 21. Gul, S.T., Khan, A., Ahmad, M. and Hussain, I. (2013) 
                  Seroprevalence of brucellosis and associated hemato-biochemical 
                  changes in Pakistani horses. Pak. J. Agric. Sci., 50(4): 
                  745-750. |  |