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R esearch
(Published online:
16-04-2015)
11.
Evaluation of lateral flow assay as a field
test for investigation of brucellosis outbreak in an organized
buffalo farm: A pilot study - R. Shome, G. Filia, B. S.
Padmashree, N. Krithiga, Swati Sahay, K. Triveni, B. R. Shome, V.
Mahajan, Amarjit Singh and H. Rahman
Veterinary World, 8(4): 492-496
doi:
10.14202/vetworld.2015.492-496
R.
Shome: Bacteriology
Lab-1, National Institute of Veterinary Epidemiology and Disease
Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka,
Bengaluru - 560 064, Karnataka, India;
rajeswarishome@gmail.com
G.
Filia: Animal Disease
Research Centre, College of Veterinary Science, Guru Angad Dev
Veterinary and Animal Sciences University,
Ludhiana - 141 004, Punjab,
India; harpalfilia@rediffmail.com
B. S.
Padmashree: Bacteriology
Lab-1, National Institute of Veterinary Epidemiology and Disease
Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka,
Bengaluru - 560 064, Karnataka, India;
padmashreebioscience@gmail.com
N.
Krithiga: Bacteriology
Lab-1, National Institute of Veterinary Epidemiology and Disease
Informatics (Formerly PD_ADMAS), Ramagondanahalli, Yelahanka,
Bengaluru - 560 064, Karnataka, India;
krithigavet_83@yahoo.co.in
Swati
Sahay: Bacteriology
Lab-1, National Institute of Veterinary Epidemiology and Disease
Informatics (Formerly PD_ADMAS),
Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka,
India; simi17kol@gmail.com
K.
Triveni: Bacteriology
Lab-1, National Institute of Veterinary Epidemiology and Disease
Informatics (Formerly PD_ADMAS),
Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka,
India; trivenichat@gmail.com,
B. R.
Shome: Bacteriology
Lab-1, National Institute of Veterinary Epidemiology and Disease
Informatics (Formerly PD_ADMAS),
Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka,
India; brshome@gmail.com
V.
Mahajan: Animal Disease
Research Centre, College of Veterinary Science, Guru Angad Dev
Veterinary and Animal Sciences University, Ludhiana - 141 004,
Punjab, India; mahajanv17@gmail.com
Amarjit
Singh: Animal Disease
Research Centre, College of Veterinary Science, Guru Angad Dev
Veterinary and Animal Sciences University, Ludhiana - 141 004,
Punjab, India; amarjitsingh64@gmail.com
H.
Rahman: Bacteriology Lab-1, National Institute of Veterinary
Epidemiology and Disease Informatics (Formerly PD_ADMAS),
Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka,
India; hricar@gmail.com
Received: 21-10-2014, Revised: 01-03-2015, Accepted: 12-03-2015,
Published online: 16-04-2015
Corresponding author:
Rajeswari Shome, e-mail: rajeswarishome@gmail.com
Citation:
Shome R, Filia G,
Padmashree BS, Krithiga N, Sahay S, Triveni K, Shome BR, Mahajan
V, Singh A, Rahman H (2015) Evaluation of lateral flow assay as a
field test for investigation of brucellosis outbreak in an
organised buffalo farm: A pilot study, Veterinary World 8(4):
492-496.
Abstract
Aim:
The aim was to evaluate lateral flow assay (LFA) as a field
test for investigation of brucellosis outbreak in organized
buffalo farm.
Materials and Methods: A total of 153 serum samples were
tested to detect the presence of brucella antibodies by LFA
and three other serological tests i.e. rose bengal plate test (RBPT),
protein G based indirect enzyme-linked immunoassay (iELISA), and
competitive ELISA (cELISA). The performances of LFA and other
serological tests were evaluated using OIE complaint cELISA as the
gold standard.
Results: Serological tests revealed 50% of the animals were
seropositive for Brucella antibodies and correlated with
clinical history of abortions, infertility, and productive
failures. The newly developed assay showed 87.1% and 92.6%
sensitivity and specificity, which was even higher than the
specificity of RBPT.
Conclusions: The investigation proved the potential usefulness
of LFA for field diagnosis of brucellosis in the regions where
laboratory facilities are limited.
Keywords: brucellosis outbreak, competitive enzyme-linked
immunoassay, indirect enzyme-linked immunoassay, lateral flow
assay, rose bengal plate test.
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