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R esearch
(Published online:
24-04-2015)
19.
Single nucleotide markers of D-loop for
identification of Indian wild pig (Sus scrofa cristatus)
- Gaurav
Kumar Srivastava, Nidhi Rajput, Kajal Kumar Jadav, Avadh Bihari
Shrivastav and Himanshu R. Joshi
Veterinary World, 8(4): 532-536
doi:
10.14202/vetworld.2015.532-536
Gaurav
Kumar Srivastava:
Centre for Wildlife Forensic and Health, Nanaji Deshmukh
Veterinary Science University, Jabalpur, Madhya Pradesh, India;
gauravsrivastava@gmail.com
Nidhi
Rajput:
Centre for Wildlife Forensic and Health, Nanaji Deshmukh
Veterinary Science University, Jabalpur, Madhya Pradesh, India;
nidhi3rajput@gmail.com
Kajal
Kumar Jadav:
Centre for Wildlife Forensic and Health, Nanaji Deshmukh
Veterinary Science University, Jabalpur, Madhya Pradesh, India;
kaju364@rediffmail.com
Avadh
Bihari Shrivastav:
Centre for Wildlife Forensic and Health, Nanaji Deshmukh
Veterinary Science University, Jabalpur, Madhya Pradesh, India;
drabshrivastav@yahoo.co.in
Himanshu R. Joshi: Centre for Wildlife Forensic and Health,
Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya
Pradesh, India;
himanshu.joshi87@gmail.com
Received:
28-12-2014, Revised: 21-03-2015, Accepted: 28-03-2015, Published
online: 24-04-2015
Corresponding author:
Kajal Kumar Jadav, e-mail: KKJ: kaju364@rediffmail.com
Citation:
Srivastava GK, Rajput N, Jadav KK, Shrivastav
AB, Joshi HR (2015) Single nucleotide markers of D-loop for
identification of Indian wild pig (Sus scrofa cristatus),
Veterinary World 8(4); 532-536.
Abstract
Aim:
Partial fragment of D-loop region extending from 35 to 770
were compared with corresponding sequences of 16 wild pigs and 9
domestic pig breeds from different parts of the world for
detection of single nucleotide polymorphism (SNP) markers in the
region. The paper also reappraises SNP markers from two fragments
of cytochrome b gene and a fragment 12S rRNA gene distinguishing
the Indian wild pig from other pig species of the world.
Materials and Methods: Deoxyribonucleic acid (DNA) was
isolated from 14 and 12 tissue samples of Indian wild and domestic
pigs, respectively, collected from Central India for
characterization of the D-loop DNA sequences using universal
primers. The sequences obtained were aligned along with the
retrieved sequences to analyze species-specific SNP marker.
Results: A total of 58 mitochondrial D-loop
gene sequences of pig races were aligned to identify 1349
polymorphic sites in the fragment from nucleotide positions 35-770
bp and four SNP markers were identified to differentiate Indian
wild pig from all the sequences investigated in this study. With
the inclusion of cytochrome b gene and 12S rRNA gene fragments,
the present study contributes to the total 15 SNP markers, which
have been identified in the mitochondrial fragment of 1936 bp for
identification of Indian wild pig.
Conclusion: SNP markers have advantages over other marker
types and do not require subsequent standardization to compare
data across studies or laboratories.
Keywords: D-loop, Indian wild pig, single
nucleotide markers, species identification.
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