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R esearch
(Published online:
07-04-2015)
2.
Diagnosis of peste des petits ruminants infection in small
ruminants through in-house developed Indirect ELISA: Practical
considerations
-
K. K.
Sharma, D. P. Kshirsagar, I. H. Kalyani, D. R. Patel, P. D. Vihol
and J. M. Patel
Veterinary World, 8(4): 443-448
doi:
10.14202/vetworld.2015.443-448
K. K. Sharma:
Department of Veterinary Microbiology,
Vanbandhu College of Veterinary Science and Animal Husbandry,
Navsari Agricultural University, Navsari, Gujarat, India;
kishan12sharma@rediffmail.com
D. P. Kshirsagar:
Department of
Veterinary Public Health and Epidemiology, Vanbandhu College of
Veterinary Science and Animal Husbandry, Navsari Agricultural
University, Navsari, Gujarat, India;
drdpk04v@gmail.com
I. H. Kalyani:
Department of Veterinary
Microbiology, Vanbandhu College of Veterinary Science and Animal
Husbandry, Navsari Agricultural University, Navsari, Gujarat,
India; ihkvet@gmail.com
D. R. Patel:
Department of Veterinary Microbiology,
Vanbandhu College of Veterinary Science and Animal Husbandry,
Navsari Agricultural University, Navsari, Gujarat, India;
vetdharmesh74@yahoo.com
P. D. Vihol:
Department of Veterinary Pathology,
Vanbandhu College of Veterinary Science and Animal Husbandry,
Navsari Agricultural University, Navsari, Gujarat, India; drpriti@nau.in
J. M. Patel:
Department of Veterinary Pathology,
Vanbandhu College of Veterinary Science and Animal Husbandry,
Navsari Agricultural University, Navsari, Gujarat, India; dr.jams@rediffmail.com
Received:
26-11-2014, Revised: 25-02-2015, Accepted: 05-03-2015, Published
online: 07-04-2015
Corresponding author:
D. P. Kshirsagar, e-mail:
drdpk04v@gmail.com
Citation:
Sharma KK, Kshirsagar DP, Kalyani IH, Patel DR, Vihol PD, Patel JM
(2015) Diagnosis of peste des petits ruminants infection in small
ruminants through in-house
developed Indirect ELISA: Practical considerations,
Veterinary World 8(4): 443-448.
Abstract
Aim: The work was conducted
to diagnose peste des petits ruminants (PPR) outbreak through an
in house developed indirect ELISA (thereafter referred as iELISA)
its comparison with other available diagnostic tests and
description of practical considerations in its development,
utility and limitations.
Materials and Methods: An outbreak resembled to PPR occurred
in two different places of southern Gujarat viz. Vapi and
Navsari, affecting 622 animals, including both goat (n = 476) and
sheep (n = 146). Animals displayed the typical signs of PPR at
Vapi; however diarrhea was the inconsistent feature in animals of
Navsari. The affection caused morbidity of 100% and mortality were
73.68% (n = 392/532) and 56.67% (n = 51/90) in Vapi and Navsari
outbreaks, respectively. Relevant ante mortem and post mortem
samples were collected from representative animals. At the outset
of the epidemic no kit was available with us, so agar gel
immunodiffusion (AGID) was carried out and a commercial ELISA (cELISA)
kit was ordered for making diagnosis through antibody
demonstration. Meanwhile, an iELISA was developed in house using
PPR vaccine as antigen and protein G conjugated HRPO antibody as
detector. Histopathology and results of sandwich ELISA were also
used to diagnose PPR virus (PPRV) in the outbreak.
Results: The iELISA developed had detected PPRV antibodies in
22/24 samples (91.66%). Significant difference was observed in
disease sensitivity pattern of two species by Chi-square test.
While AGID failed to detect antibodies in any sample. Results were
reconfirmed by comparing with commercially available cELISA kit.
Conclusion: PPR is an economically important disease and for
the rapid diagnosis of PPR the in house developed antibody capture
iELISA can be a suitable cost effective alternative.
Keywords: agar gel immunodiffusion, competitive ELISA, Goat,
indirect ELISA, peste des petits ruminants.
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