Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
R esearch
(Published online:
24-04-2015)
20.
Hepatoprotective activity of Moringa
oleifera against cadmium toxicity in rats -
Reetu Toppo, Birendra
Kumar Roy, Ravuri Halley Gora, Sushma Lalita Baxla and Prabhat
Kumar
Veterinary World, 8(4): 537-540
doi:
10.14202/vetworld.2015.537-540
Reetu
Toppo:
Department of Veterinary Pharmacology & Toxicology, College of
Veterinary Science & A. H., Birsa Agricultural University,
Ranchi -
834 006, Jharkhand, India; drreetuoraon@gmail.com
Birendra Kumar Roy:
Department of Veterinary Pharmacology & Toxicology, College of
Veterinary Science & A. H., Birsa Agricultural University, Ranchi
- 834 006, Jharkhand, India;
roybk2001@yahoo.co.in
Ravuri
Halley Gora:
Department of Veterinary Pharmacology & Toxicology, College of
Veterinary Science & A. H., Birsa Agricultural University,
Ranchi -
834 006, Jharkhand, India; halleygora@gmail.com
Sushma
Lalita Baxla:
Department of Veterinary Pharmacology & Toxicology, College of
Veterinary Science & A. H., Birsa Agricultural University, Ranchi
- 834 006, Jharkhand, India;
sushmalbaxla@gmail.com
Prabhat
Kumar: Department of Veterinary Pharmacology & Toxicology,
College of Veterinary Science & A. H., Birsa Agricultural
University,
Ranchi -
834 006, Jharkhand, India;
guddunalanda@gmail.com
Received:
04-12-2014, Revised: 23-03-2015, Accepted: 28-03-2015, Published
online: 24-04-2015
Corresponding author:
Ravuri Halley Gora, e-mail: halleygora@gmail.com
Citation:
Toppo R, Roy BK, Gora RH, Baxla SL, Kumar P
(2015) Hepatoprotective activity of Moringa oleifera
against cadmium toxicity in rats,
Veterinary World 8(4); 537-540.
Abstract
Aim:
The present investigation has been conducted to evaluate the
hepatoprotective activity of Moringa oleifera against
cadmium-induced toxicity in rats.
Materials and Methods: For this study, 18 Wistar albino rats
were taken. Control group, Group I rats were given cadmium
chloride @ 200 ppm per kg and Group II rats were treated with
M. oleifera extract @ 500 mg/kg along with cadmium chloride @
200 ppm per kg (daily oral for 28 days). On 29 th
day, animals were slaughtered and various
parameters were determined. Serum biomarkers, oxidative stress
parameters, histomorphological examination were carried out with
estimation of cadmium concentration in liver tissues.
Results: Oral administration of cadmium chloride @ 200 ppm/kg
for 28 days resulted in a significant increase in aspartate
aminotransferase (AST), alanine transaminase (ALT), alkaline
phosphatase (ALP), significant (p ≤0.01)
increase of lipid peroxidation (LPO) and decrease in superoxide
dismutase (SOD), and increase in cadmium accumulation in liver.
Treatment with M. oleifera @ 500 mg/kg significantly
(p<0.01) decreased the elevated ALP, AST, ALT, LPO levels and
increase in SOD levels, and as compared to cadmium chloride
treated group. However, there was no significant difference in
cadmium concentration in liver when compared with cadmium chloride
treated group.
Conclusion: The study conclude that supplementation of M.
oleifera (500 mg/kg), daily oral for 28 days has shown
protection against cadmium-induced hepatotoxicity.
Keywords: cadmium, Moringa oleifera,
rats, serum bio-markers, toxicity.
References
1. Patrick, L. (2003) Toxic metals and antioxidants: Part II:
The role of antioxidants in arsenic and cadmium toxicity.
Altern. Med. Rev., 8(2):106-128.
PMid:12777158 |
|
|
|
2. Mehana, E.S. (2008). Pathological and clinico-pathological
studies on the protective effect of vitamin E against cadmium
chloride toxicosis in male albino rats. Egypt. J. Comp. Path.
Clin. Path., 21(3): 32-52. |
|
|
|
3. Tarasub, N., Devakul, N. and Ayutthaya, W. (2008) Effect of
curcumin on cadmium-induced hepatotoxicity in rats. Thai. J.
Toxicol., 23(2): 100-107. |
|
|
|
4. Ajilore, B.S. and Olugbenga, A. (2012) Hepatoprotective
potentials of methanolic extract of the leaf of Momordica
charantia linn on cadmium induced hepatotoxicity in rats. J.
Nat. Sci. Res., 2(7): 41-47. |
|
|
5. Amara, S., Douki, T., Garrel, C., Favier, A., Rhouma, K.,
Sakly, M. and Abdelmelek, H. (2011) Effect of static magnetic
field and cadmium on oxidative stress and DNA damage in rat
cortex brain and hippocampus. Toxicol. Indian. Health., 27(2):
99-106.
http://dx.doi.org/10.1177/0748233710381887
PMid:20837562 |
|
|
|
6. Davidson-Hunt, I. (2000) Ecological ethno botany: Stumbling
toward new practices and paradigms. MASA J., 16: 1-13. |
|
|
|
7. Sinha, M., Das, D.K., Datta, S., Gosh, S. and Dey, S.
(2011) Amelioration of ionizing radiation induced lipid
peroxidation in mouse liver by Moringa oleifera Lam. Leaf
extract. Indian. J. Exp. Biol., 50(3): 209-215. |
|
|
|
8. Onyekaba1, T.U., Chinedu, O.G. and Fred, A.C. (2013)
Phytochemical screening and investigations of antibacterial
activities of various fractions of the ethanol leaves extract
of Moringa oleifera LAM (Moringaceae). J. Pharm. Chem. Biol.
Sci., 3(3): 962-973. |
|
|
|
9. Anwar, F. and Rashid, U. (2007) Physiochemical
characteristics of Moringa oleifera seeds and seed oil from a
wild provenance of Pakistan. Pak. J. Bot., 39: 1443-1453. |
|
|
|
10. Walter, A. (2011) Antibacterial activity of Moringa
oleifera and Moringa stenopetala methanol and n-hexane seed
extracts on bacteria implicated in water-borne diseases. Afr.
J. Microbiol. Res., 51: 153-157. |
|
|
|
11. Paliwal, R. (2011) Elucidation of free radical scavenging
and antioxidant activity of aqueous and hydro-ethanolic
extracts of Moringa oleifera pods. Res. J. Pharm. Tech., 4:
566-571. |
|
|
|
12. Nikkon, F. (2003) In vitro antimicrobial activity of the
compound isolated from chloroform extract of Moringa oleifera
Lam. Pak. J. Biol. Sci., 22: 1888-1890. |
|
|
|
13. Gehan, A.E., El-Emery and Ayman, Y.A. (2010) Cadmium
ginger two way antagonistic relationship. Arab. J. Biotech.,
13(1): 115-124. |
|
|
|
14. Kumar, C.S., Balamurugan, B., Merugeswaran, S., Natarajan,
P., hHaravanan, S.P., Petchimuthu, S and Murungan, T.S. (2010)
Protective activity of leaves and roots extracts of Moringa
Oleifera Lam. Int. J. Medicobiol. Res., 1(2): 90-93. |
|
|
15. Madesh, M. and Balasubramanian, K.A. (1998) Microtiter
plate assay for superoxide dismutase using MTT reduction by
superoxide. Indian. J. Biochem. Biophys., 35(3): 184-188.
PMid:9803669 |
|
|
16. Rehman, S.U. (1984) Lead induced regional lipid
peroxidation in brain. Toxicol. Lett., 21(3): 333-337.
http://dx.doi.org/10.1016/0378-4274(84)90093-6 |
|
|
17. Datta, B.K., Mishra, A., Singh, A., Sar, T.K., Sarkar, S.,
Bhatacharya, A., Chakraborty, A.K. and Mandal, T.K. (2010)
Chronic arsenicosis in cattle with special reference to its
metabolism in arsenic endemic village of Nadia district West
Bengal India. Sci Total Environ, 409(2): 284-288.
http://dx.doi.org/10.1016/j.scitotenv.2010.10.003
PMid:21059470 |
|
|
18. Stillman, M.J. and Zelazowski, A.J. (1988) Domain
specificity in metal binding to metallothi – Onein. A circular
dichroism and magnetic circular dichroism study of cadmium and
zinc binding at temperature extremes. J. Biol. Chem., 263(13):
6128-6133.
PMid:3360778 |
|
|
19. Habeebu, J., Liu, J. and Klaassen, C.D. (1998)
Cadmium-induced apoptosis in mouse liver. Toxicol. Appl.
Pharmacol., 149: 203-209.
http://dx.doi.org/10.1006/taap.1997.8334
PMid:9571989 |
|
|
20. Naik, P. (2010) Biochemistry. 3rd ed. Jaypee Publishers
Ltd, Panama. p138-141, 565.
http://dx.doi.org/10.5005/jp/books/11094 |
|
|
|
21. Mauro, P. and Renze, B. (2008) In: Fundamentals of
Clinical Chemistry. 6th ed. p325. |
|
|
22. Haidry, M.T. and Malik. A. (2014) Hepatoprotective and
antioxidative effects of Terminalia arjuna against cadmium
provoked toxicity in Albino rats (Ratus norvigieus). Biochem.
Pharmacol., 3: 1.
http://dx.doi.org/10.4172/2167-0501.1000130 |
|
|
23. Ibiam, A.U., Ugwuja, E.I., Ejeogo, C. and Ugwu, O. (2013)
Cadmium induced toxicity and the hepatoprotective potentials
of aqueous extract of Jessiaca nervosa leaf. Adv. Pharm
Bulletin., 3(2): 309-313.
PMid:24312853 PMCid:PMC3848217 |
|
|
|
24. Rajasekaran, A. and Periasamy, M. (2012) Hepatoprotective
effect of ethanolic leaf extract of Calycopteris floribunda
lam on cadmium induced hepatotoxicity in rats. Res. J. Pharm.
Biol. Chem. Sci., 3(3): 382-390. |
|
|
|
25. Gaurav, D., Preet, S. and Dua, K.K. (2011) Protective
influence of dietary nutrients on antioxidant defense system
in the blood of rats treated with cadmium. Adv. Appl. Sci.
Res., 2(2): 69-78. |
|
|
26. Uchida, H., Kurata, Y., Hiratsuka, H. and Umemura,
T.(2010) The effect of vitamin D deficient diet on chronic
cadmium exposure in rats. Toxicol. Pathol., 38(5): 730-737.
http://dx.doi.org/10.1177/0192623310374328
PMid:20622193 |
|
|
|
27. Kumar, P.V., Pricy, A.A., Kumar, C.S. and Goud, G.K.K.
(2010) Hepatoprotective effect of green tea (Camellia sinesis)
on cadmium chloride induced toxicity in rats. J. Chem. Pharm.
Res., 2(6): 125-128. |
|
|
|
28. Ibrahim, N.K. (2013) Possible protective effect of
kombucha tea ferment on cadmium chloride induced liver and
kidney damage in irradiated rats Int. J. Biol. Life Sci., 9:
1. |
|
|
29. Pari, L. and Karthikesan, K. (2007) Protective role of
caffeic acid against alcohol – Induced biochemical changes in
rats. Fundam Clin Pharmacol., 21(4): 355-361.
http://dx.doi.org/10.1111/j.1472-8206.2007.00505.x
PMid:17635173 |
|
|
|
30. Selvakumar, D. and Natarajan, P. (2008) Hepato-protective
activity of Moringa oleifera Lam. leaves in carbon
tetrachloride induced hepatotoxicity in albino rats.
Pharmacogn. Mag., 4: 97-98. |
|
|
|
31. Mitra, E., Ghosh, A.K., Ghosh, D., Firdaus, S.Y.,
Mukherjee, D., Chattopadhya, A., Pattari, S.K., Dutta, S. and
Bandyopadhya, D.(2013) Ameliorative effect of aqueous curry
leaf (Murraya Koenigi) extract against cadmium induced
oxidative stress in rat liver: Involvement of antioxidant
mechanisms. Int. J. Pharm. Pharm. Sci., 5(2): 570-583. |
|
|
|
32. Esther, A.O., Funnmilola, O.Y., Violette, A.N. and
Oziegbe, O.O. (2013) Effect of African Walnut (Tetracarpidium
conophorum (Mull. Arg.). Hutch and Dalziel syn. PlukenetI
conophora oil on cadmium – Induced oxidative stress in male
albino rats. Res. J. Pharm. Biol. Chem. Sci., 4(3): 35-42. |
|
|
33. Ognjanovic, B.I., Markovic, S.D., Pavlovic, S.Z., Zikic,
R.V., Stajn, A.S. and Saicic, Z.S. (2007) Effect of chronic
cadmium exposure on antioxidant defence system in some tissue
of rats: Protective effect of selenium. Pysiol. Res., 57:
403-411.
PMid:17465690 |
|
|
|
34. Aslam, M., Ahmad, S.T., Dayal, R., Javid, K., Umar, S.,
Asiaf, A., Nafees, S., Bhat, J.A., Wani, A., Samim, M. and
Singh, S. (2012) Nephroprotective action of Peucedanum grande
against cadmium chloride induced renal toxicity in wistar
rats. EXCLI J., 11: 444-452. |
|
|
35. Bharali, R., Tabassum, J. and Azad, M.R.H. (2003)
Chemomodulatory effect of Moringa oleifera, Lam. on hepatic
carcinogen metabolizing enzymes, antioxidant parameters and
skin papillomagenesis in mice. Asian. Pac. J. Cancer. Prev.,
4: 131-139.
PMid:12875626 |
|
|
36. Kumar, N.A. and Pari, L. (2003) Antioxidant action of
Moringa oleifera Lam. (Drumstick) against antitubercular drugs
induced lipid peroxidation in rats. J. Med. Peroxid. Food.,
6(3): 255-259.
http://dx.doi.org/10.1089/10966200360716670
PMid:14585192 |
|
|
37. Sultana, B., Anwar, F. and Ashraf, M. (2009) Effect of
extraction solvent/technique on the antioxidant activity of
selected medicinal plant extracts. Molecules., 14: 167-2180.
http://dx.doi.org/10.3390/molecules14062167
PMid:19553890 |
|
|
38. Sreelatha, S. and Padma, P.R. (2009) Antioxidant activity
and total phenolic content of Moringa oleifera leav.es in two
stages of maturity. Plant Foods Human. Nutr., 64: 303-311.
http://dx.doi.org/10.1007/s11130-009-0141-0
PMid:19904611 |
|
|
39. Massanyi, P., Tataruch, F., Slamecka, J., Toman, R. and
Jurcik, R. (2003) Accumulation of lead, cadmium, and mercury
in liver and kidney of the brown hare (Lepus europaeus) in
relation to the season, age, and sex in the West Slovakian
Lowland. J. Environ. Sci. Health., 39: 1299-1309.
http://dx.doi.org/10.1081/ESE-120021127 |
|
|
40. Hollis, L., Hogstrand, C. and Wood, C.M. (2001)
Tissue-specific cadmium accumulation, metallothionein
induction, and tissue zinc and copper levels during chronic
sublethal cadmium exposure in juvenile rainbow trout. Arch.
Environ. Contam. Toxicol., 41: 468-474.
http://dx.doi.org/10.1007/s002440010273
PMid:11598784 |
|
