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R esearch
(Published online:
09-04-2015)
5.
Effect
of species, breed, and age on bacterial load in bovine and
bubaline semen
-
Chandrahas Sannat, Ajit Nair, S. B. Sahu, S. A. Sahasrabudhe,
Ashish Kumar, Amit Kumar Gupta and R. K. Shende
Veterinary World, 8(4): 461-466
doi:
10.14202/vetworld.2015.461-466
Chandrahas Sannat:
Department of Veterinary
Microbiology, College of Veterinary Science & A.H., Chhattisgarh
Kamdhenu Vishwavidayalaya, Anjora, Durg Chhattisgarh, India;
csannat@rediffmail.com
Ajit Nair:
Central Semen Station, Livestock
Development Department, Government of Chhattisgarh, Anjora, Durg,
Chhattisgarh, India; Ajitnair1971@ gmail.com
S. B. Sahu:
Central Semen Station, Livestock
Development Department, Government of Chhattisgarh, Anjora, Durg,
Chhattisgarh, India; Sahusb@ymail.com
S. A. Sahasrabudhe:
Central Semen
Station, Livestock Development Department, Government of
Chhattisgarh, Anjora, Durg, Chhattisgarh, India;
Oic2css@gmail.com
Ashish Kumar:
Department of Veterinary Microbiology, College of Veterinary
Science & A.H., Chhattisgarh Kamdhenu Vishwavidayalaya, Anjora,
Durg Chhattisgarh, India; Ashishvetdoc@gmail.com
Amit Kumar Gupta:
Department of Veterinary
Microbiology, College of Veterinary Science & A.H., Chhattisgarh
Kamdhenu Vishwavidayalaya, Anjora, Durg Chhattisgarh, India;
Dramitkumaragrahari2009gmail.com
R. K. Shende:
Department of Veterinary Microbiology,
College of Veterinary Science & A.H., Chhattisgarh Kamdhenu
Vishwavidayalaya, Anjora, Durg Chhattisgarh, India; Rshende786@rediffmail.com
Received: 14-11-2014, Revised: 02-03-2015, Accepted: 10-03-2015,
Published online: 09-04-2015
Corresponding author:
Chandrahas Sannat, e-mail:
csannat@rediffmail.com
Citation:
Sannat
C, Nair A, Sahu SB, Sahasrabudhe SA, Kumar A, Gupta AK, Shende RK
(2015) Effect of species, breed and age on bacterial load in
bovine and bubaline semen, Veterinary World 8(4): 461-466.
Abstract
Aim: The present study was
conducted to investigate the effect of species, breed and age on
bacterial load in fresh and frozen semen of Cattle and Buffalo
bull.
Materials and Methods: Present study covered 56 cow and 10
buffalo bulls stationed at Central Semen Station Anjora, Durg
(Chhattisgarh). Impact of breeds on bacterial load in semen was
assessed using six breeds of cattle viz. Sahiwal, Gir, Red
Sindhi, Tharparkar, Jersey and Holstein Friesian (HF) cross. Cow
bulls were categorized into four different groups based on their
age (<4 years, 4-5 years, 5-6 years and > 6 years) to study
variation among age groups. Bacterial load was measured in fresh
and frozen semen samples from these bulls using the standard plate
count (SPC) method and count was expressed as colony forming unit
(CFU) per ml of semen.
Results: Higher bacterial load was reported in fresh (2.36 ×
10 4
± 1943 CFU/ml) and frozen (1.00 ×
10 ± 90 CFU/ml) semen of cow bulls as compared to buffalo bulls
(1.95 × 104
± 2882 and 7.75 × 102
± 160 CFU/ml in fresh and
frozen semen, respectively). Jersey bull showed significantly
higher bacterial count (p < 0.05) both in fresh (4.07 × 104
± 13927 CFU/ ml) and frozen
(1.92 × 103
± 178 CFU/ml) semen followed
by HF cross, Sahiwal, Gir, Red Sindhi and Tharparkar bull. Bulls
aged < 4 years and more than 6 years yielded increased bacterial
load in their semen. Although a minor variation was reported
between species and among age groups, no significant differences
were measured.
Conclusion: Bacterial load in semen did not differ
significantly between species and age groups; however significant
variation was reported among different breeds. Bulls of Jersey
breed showed significantly higher bacterial load in semen as
compared to the crossbred and indigenous bull.
Keywords: age group, bacterial load, breed, buffalo bulls, cow
bulls, semen.
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