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R esearch
(Published online:
25-08-2015)
10.
Evaluation of recombinant outer membrane protein C based indirect
enzyme-linked immunoassay for the detection of Salmonella
antibodies in poultry -
Jinu Manoj, Rajesh K.
Agarwal, Blessa Sailo, Mudasir Ahmed Wani and Manoj Kumar Singh
Veterinary World, 8(8): 1006-1010
doi:
10.14202/vetworld.2015.1006-1010
Jinu
Manoj:
Department of Veterinary Public Health and Epidemiology, College
of Veterinary & Animal Sciences, Sardar Vallabhbhai
Patel
University of Agriculture and Technology, Meerut, Uttar Pradesh,
India; drjinumanoj@rediffmail.com
Rajesh
K. Agarwal:
Division of Bacteriology and Mycology, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India;
grace_bly@yahoo.com
Blessa
Sailo:
Division of Bacteriology and Mycology, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India;
drblessavet@gmail.com
Mudasir
Ahmed Wani:
Division of Bacteriology and Mycology, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India;
drmudasirwani@gmail.com
Manoj
Kumar Singh: Department of Livestock Production and
Management, College of Veterinary & Animal Sciences, Sardar
Vallabhbhai Patel University of Agriculture and Technology,
Meerut, Uttar Pradesh, India;
drmanoj611@gmail.com
Received:
30-03-2015, Revised: 12-07-2015, Accepted: 19-07-2015, Published
online: 25-08-2015
Corresponding author:
Jinu Manoj, e-mail: drjinumanoj@rediffmail.com
Citation:
Manoj J, Agarwal RK,
Sailo B, Wani MA, Singh MK (2015) Evaluation of recombinant outer
membrane protein C based indirect enzyme-linked immunoassay for
the detection of Salmonella antibodies in poultry,
Veterinary World 8(8): 1006-1010.
Abstract
Aim:
To evaluate the efficacy of recombinant outer membrane
proteinC (rOmpC) based enzyme-linked immunoassay (ELISA) for the
diagnosis of salmonellosis in poultry.
Materials and Methods: Three antigens were prepared, and the
indirect ELISA was standardized using the antigens and the
antiserum raised in chicken against Omp and rOmpC. Sera were
collected from a total of 255 apparently healthy field chickens
and screened for the presence of Salmonella antibodies by
this ELISA.
Results: The sodium dodecyl sulfate-polyacrylamide gel
electrophoresis analysis of Omp revealed major polypeptides at 36,
42 and 52 kDa, and the rOmpC was evident by a single protein band
of 43 kDa. The Omp and rOmpC antigen revealed an optimum
concentration of 78 and 156 ng, respectively, in the assay, while
the whole cell antigen gave an optimum reaction at a concentration
of 10 6 organisms/ml. The test was found to
be specific as it did not react with any of the antisera of seven
other organisms. The developed ELISA detected Salmonella
antibodies from 22 (8.62%) samples with rOmpC antigen, while 24
(9.41%) samples gave a positive reaction with both Omp and whole
cell antigens.
Conclusion: We suggest rOmpC based indirect ELISA as a
suitable screening tool for serological monitoring of poultry
flocks.
Keywords: antibody, antigen, outer membrane
protein, poultry, Salmonella.
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