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R esearch
(Published online:
25-08-2015)
11.
Histology and scanning electron microscopy
of the tubal tonsil of goats - V. R. Indu, K. M. Lucy,
J. J. Chungath, N. Ashok and S. Maya
Veterinary World, 8(8): 1011-1014
doi:
10.14202/vetworld.2015.1011-1014
V. R.
Indu: Department of Veterinary Anatomy and Histology, College
of Veterinary and Animal Sciences, Mannuthy, Kerala
Veterinary
and Animal Sciences University, Kerala, India;
drinduvraj@yahoo.com
K. M.
Lucy: Department of Veterinary Anatomy and Histology, College
of Veterinary and Animal Sciences, Mannuthy, Kerala
Veterinary
and Animal Sciences University, Kerala, India;
lucy@kvasu.ac.in
J. J.
Chungath: Department of Veterinary Anatomy and Histology,
College of Veterinary and Animal Sciences, Mannuthy, Kerala
Veterinary
and Animal Sciences University, Kerala, India;
jose@kvasu.ac.in
N.
Ashok: Department of Veterinary Anatomy and Histology, College
of Veterinary and Animal Sciences, Mannuthy, Kerala
Veterinary
and Animal Sciences University, Kerala, India;
ashokn@kvasu.ac.in
S. Maya:
Department of Veterinary Anatomy and Histology, College of
Veterinary and Animal Sciences, Pookode, Kerala Veterinary and
Animal Sciences University, Kerala, India;
maya@kvasu.ac.in
Received:
14-05-2015,
Revised:
17-07-2015,
Accepted:
24-07-2015,
Published online:
25-08-2015
Corresponding author:
V. R. Indu, e-mail: drinduvraj@yahoo.com
Citation:
Indu VR, Lucy KM,
Chungath JJ, Ashok N, Maya S (2015) Histology and scanning
electron microscopy of the tubal tonsil of goats, Veterinary
World 8(8): 1011-1014.
Abstract
Aim:
To observe the light and scanning electron microscopy (SEM) of
the caprine tubal tonsil.
Materials and Methods: The study was conducted on six
crossbred male goats of 6 months of age. From the median sections
of the head, tissue pieces from the nasopharynx around the
auditory tube were collected and fixed for histology and SEM.
Results: Tonsillar lymphoid tissue was located in the
nasopharynx ventral to the auditory tube opening in the lateral
wall of the pharynx. The height of the surface epithelium of the
tubal tonsil measured 80.17±1.08 μm and was a pseudostratified
ciliated columnar type with basal, supporting, and goblet cells.
Above the dome of lymphoid nodules, the epithelium was modified
into a follicle associated epithelium (FAE), also called lympho-epithelium
or reticular epithelium and was characterized by the absence of
goblet cells and cilia, reduced number of cell layers, and a large
number of lymphoid cells due to interrupted basement membrane. The
height of FAE was smaller than that of the surface epithelium and
measured 34.33±0.92 μm. The surface of tubal tonsil showed folds
and invaginations, which formed crypts. The lamina
propria-submucosa underneath the epithelium was formed by the
meshwork of reticular and, thin and loose collagen fibers with
dome-like accumulation of lymphoid nodules. In the secondary
lymphoid nodules, a corona, parafollicular area, and interfnodular
area were observed. The average number of lymphoid nodules counted
per field under low power magnification of microscope was
1.17±0.17, and the internodular distance was 34.00±4.37 μm. The
mean diameter of lymphoid nodules was 566.67±11.45 μm and the
lymphocyte count per nodule was 14741.67±174.36. The number of
plasma cells counted per field under low power was 44.38±2.90
below the surface epithelium. The tubal tonsil was not
encapsulated. In SEM, the surface epithelium of the tubal tonsils
presented ciliated cells, microvillus (MV) cells, and goblet
cells. The region of FAE possessed Type-I and Type-II MV cells and
microfold (M) cells in between.
Conclusion: It was concluded that the tubal
tonsils were well developed in goats, which might serve as a means
of protection against the spread of infection to the middle ear
cavity.
Keywords: goats, histology, tubal tonsil.
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