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R esearch
(Published online:
26-08-2015)
14.
Effect of glutamine supplementation and
replacement of tris-egg yolk based extender with defatted cow milk
on spermatozoa quality after equilibration and thawing -
Vasundhara Dawra, Brijesh Yadav and Sarvajeet Yadav
Veterinary World, 8(8): 1027-1031
doi:
10.14202/vetworld.2015.1027-1031
Vasundhara Dawra:
Department of Veterinary Physiology, College of Veterinary Science
and Animal Husbandry, Pt. Deen Dayal Upadhyaya
Pashu
Chikitsa Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura,
Uttar Pradesh, India; dawravasu@gmail.com
Brijesh Yadav:
Department of Veterinary Physiology, College of Veterinary Science
and Animal Husbandry, Pt. Deen Dayal Upadhyaya
Pashu
Chikitsa Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura,
Uttar Pradesh, India; drbrijvet@gmail.com
Sarvajeet Yadav: Department of Veterinary Physiology, College
of Veterinary Science and Animal Husbandry, Pt. Deen Dayal
Upadhyaya
Pashu
Chikitsa Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura,
Uttar Pradesh, India;
yadav_s24@hotmail.com
Received: 19-04-2015, Revised: 23-07-2015, Accepted: 29-07-2015,
Published online: 26-08-2015
Corresponding author:
Brijesh Yadav, e-mail: drbrijvet@gmail.com
Citation:
Dawra V, Yadav B,
Yadav S (2015) Effect of glutamine supplementation and replacement
of tris-egg yolk based extender with defatted cow milk on
spermatozoa quality after equilibration and thawing, Veterinary
World 8(8): 1027-1031 .
Abstract
Aim:
The present study was designed to evaluate the effect of
supplementation of glutamine and replacement of Tris-egg yolk (TE)
based buffer with defatted cow milk on the spermatozoa quality
after equilibration and thawing.
Materials and Methods: Semen was collected from five Bhadawari
bulls biweekly, and a total of 30 ejaculates were taken. The semen
ejaculates were pooled and divided into three equal parts. The
pooled semen was diluted by TE based extender (control), TE +
glutamine (8 mM) (T1) and 50% TE + 50% deffated cow milk +
glutamine (8 mM) (T2). At two stages viz. after
equilibration and after 12 h of cryopreservation (thawed samples),
progressive motility, percent live, and percent acrosomal damage
of the spermatozoa was assessed.
Results: Supplementation of glutamine improved (p<0.05) the
spermatozoa quality with respect to the progressive motility,
percent live and acrosomal damage both post-equilibration and
post-thaw. T2 improved (p<0.05) the spermatozoa quality as
compared to control, however; it was less (p<0.05) effective as
compared T1 both post-equilibration and post-thaw.
Conclusion: From the results of present
study it can be concluded that glutamine supplementation was
effective in maintaining post-equilibration and post-thaw
spermatozoa quality whereas defatted cow milk was not as effective
as TE based buffer in the extender in improving the spermatozoa
quality.
Keywords: Bhadawari bull, defatted milk,
glutamine, post-thaw spermatozoa quality.
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