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Research (Published online: 09-08-2015)

4 Tissue reactivity and suture handling characteristics of “jimat” against silk and chromic gut in cat thigh muscle: A comparative study - Tilahun Bekele, A. P. Bhokre and Abreha Tesfaye

Veterinary World, 8(8): 958-969



   doi: 10.14202/vetworld.2015.958-969


Tilahun Bekele: School of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Jimma University, P. O. Box 307, Jimma, Ethiopia;

A. P. Bhokre: Department of Veterinary Medicine, College of Veterinary Medicine, Mekelle University, P. O. Box 231, Mekelle, Ethiopia;

Abreha Tesfaye: Department of Veterinary Medicine, College of Veterinary Medicine, Mekelle University, P. O. Box 231, Mekelle, Ethiopia;


Received: 31-03-2015, Revised: 03-07-2015, Accepted: 12-07-2015, Published online: 09-08-2015


Corresponding author: Tilahun Bekele, e-mail:

Citation: Bekele T, Bhokre AP, Tesfaye A (2015) Tissue reactivity and suture handling characteristics of “jimat” against silk and chromic gut in cat thigh muscle: A comparative study, Veterinary World 8(8): 958-969.

Aim: This study was conducted to evaluate and compare the tissue reactivity and suture handling characteristics of chromic gut, silk, and ‘jimat’ suture materials in cat thigh muscle.

Materials and Methods: This experimental study was conducted from November, 2013 to April, 2014 in Kombolcha Animal Diseases Survey, Research and Diagnostic Laboratory, Kombolcha, Ethiopia. A total of 36 local breed male cats were randomly assigned into chromic gut, silk, and “jimat” groups of 12 cats each as A, B, and C, respectively. The hind leg muscle biceps femoris was incised and sutured with suture materials according to their groups. The muscle samples with its suture were collected at six different days interval i.e. 1, 3, 7, 14, 21, and 28 and processed histopathologically to assess the degree of leukocytic infiltration and fibrous and granulation tissue formation (GTF). In addition, all suture materials were evaluated intraoperatively about their handling characteristics, by rating the precision of knot tying, square knot positioning, and resistance to knot slippage. The statistical analysis was done with two-way ANOVA, Kruskal–Wallis, and Chi-square tests.

Results: The histopathology showed that “jimat” thread (2.41.2) had produced least leukocytic infiltration than chromic gut (4.51.9) and silk (4.31.5) sutures during the study period. Higher GTF was seen at day 3 (6 [100%]), 7 (6 [100%]) and day 14 (4 [66.7%]) in all sutures, whereas “jimat” showed significantly (p<0.05) higher fibrous tissue formation (10 [83.3%]) than others. Moreover, “jimat” suture had equal suture handling characteristics (p>0.05) with both chromic gut and silk.

Conclusion: The result indicated that a single strand “jimat” thread appears to be the most satisfactory suture material as regards to both tissue reaction and suture handling characteristics for skeletal muscle approximation in cats and provided that studies on its carcinogenic effects should be done.

Keywords: handling characteristics, histopathology, leukocytic infiltration, sutures, thigh muscle.

1. Parell, G. and Becker, G. (2003) Comparison of absorbable with nonabsorbable sutures in closure of facial skin wounds. Arch. Facial Plast. Surg., 5: 488-490.
2. Abdessalem, F., Debbai, F., Jedda, H., Elmarzougui, S. and Mokhtar, S. (2009) Tensile and knot performance of polyester braided sutures. Text. Res. J., 79(3): 247-252.
3. Greenberg, J. (2010) The use of barbed sutures in obstetrics and gynecology. Rev. Obstet. Gynecol., 2(2): 82-91.
4. Fossum, T., Hedlund, C.S. and Johnson, A.L. (1997) Small Animal Surgery. 2nd ed. Mosby, St. Louis, USA. p42.
5. Bennett, R., Y'aeger, M., Trapp, A. and Cambre, R. (1997) Histologic evaluation of the tissue reaction to five suture materials in the body wall of rock doves (Columba livia). J. Avian Med. Surg., 11: 175-182.
6. Dunn, D. (2007) Wound Closure Manual. Ethicon: A Johnson and Johnson Company, USA. p9-40.
7. Bennett, R. (1988) Continuing medical education: Selection of wound closure materials. J. Am. Acade. Dermatol., 18: 619-635.
8. Fossum, T. (2002) Biomaterials, suturing, and hemostasis. In: Fossum, T.W., Hedlund, C.S. and Johnson, A.L. editors. Small Animal Surgery. 2nd ed. Mosby Publishing, St. Louis, MO. p43-59.
9. Hu, X. and Yang, H. (2000) Polyamide and polyester fibers. J. Compos. Mater., 1: 227-244.
10. Hochberg, J., Meyer, K. and Marion, M. (2009) Suture choice and other methods of skin closure. Surg. Clin. N. Am., 89: 627-641.
11. Meyer, R. and Antonini, C. (1989) A review of suture materials, Part II. In: Compendium of CME in dentistry. Compendium., 10: 360-368.
12. Runk, A., Allen, S. and Mahaffey, E. (1999) Tissue reactivity to poliglecaprone 25 in the feline lineaalba. Vet. Surg., 28: 466-471.
13. Cantini, M. and Carraro, U. (1995) Macrophage-released factor stimulates selectively myogenic cells in primary muscle culture. J. Neuropath. Exp. Neur., 54: 121-128.
14. Tidball, J. (2005) Inflammatory processes in muscle injury and repair. Am. J. Physiol-Reg. I., 288: R345-R353.
15. Sadove, A., Van Aalst, J. and Culp, J. (2004) Right palate repair: Art and issues. Clin. Plast Surg., 21: 221-241.
16. Gusman, N. (2012) Lower extremity soft tissue and cutaneous plastic surgery: Suture materials and techniques. Available from: Last accessed on 05-09-2014.
17. Bizuwork, A., Kebede, N., Tibat, T., Tilahun, G. and Kassa, T. (2013) Occurrences and financial significance of bovine cystic echinococcosis in Southern Wollo, Northeastern Ethiopia. J. Vet. Med. Anim. Health, 5(2): 51-56.
18. Driscoll, G., Baird, P., Merkelbach, P. and Smith, D. (1982) Synthetic absorbable and nonabsorbable microsutures: A histological comparison. Clin. Reprod. Fertil., 3: 151-156.
19. Schoenenberger, A., Mettler, D., Roesler, H., Zimmermann, A., Bilweis, J., Schilt, W. and Zingg, E. (1985) Surgical repair of the kidney after blunt lesions of intermediate degree using a Vicryl mesh: An experimental study. J. Urol., 134: 804-808.
20. Miro, D., Julia, M. and Sitges-Serra, A. (1995) Wound breaking strength and healing after suturing non-injured tissues. J. Am. Coll. Surg., 180: 659-665.
21. Wainstein, M., Anderson, J. and Elder, J. (1997) Comparison of effects of suture materials on wound healing in a rabbit pyeloplasty model. Urology, 49(2): 261-264.
22. Bhargava, D., Anantanarayanan, P., Prakash, G., Jayachandran, B. and Deshpande, A. (2013) Initial inflammatory response of skeletal muscle to commonly used suture materials: An animal model study to evaluate muscle healing after surgical repair – Histopathological perspective. Med. Oral Patol. Oral, 18(3): e491-e496.
23. Papazoglou, L., Tsioli, V., Papaioannou, N., Georgiadis, M., Savvas, L., Prassinos, N., Kouti, V., Bikiaris, D., Hadzigiannakis, C. and Zavros, N. (2010) Comparison of absorbable and nonabsorbable sutures for intradermal skin closure in cats. Can. Vet. J., 51: 770-772.
PMid:20885834 PMCid:PMC2885123
24. Powers, M., Beck, B. and Fonseca, R. (1997) Management of soft tissue injuries. In: Fonseca, R.J., Walker, R.V. and Betts, N.J. editors. Oral and Maxillofacial Trauma. Philadelphia, PA: WB Saunders Co., USA. p792-854.
25. Matičić, D., Kreszinger, M., Pirkić, B., Vnuk, D., Radišić, B. and Gračner, D. (2005) Comparative study of skin closure in dogs with polypropylene and polyglactin 910. Vet. Arch., 75(5): 282-290.
26. Durmus, M. (2002) The effects of single-dose dexamethasone on wound healing in rats. Anesth. Analg., 97: 1277-1280.
27. Takahashi, M., Umehara, N., Suzuki, S. and Tezuka, M. (2001) Analgesic action of a sustained release preparation of diclofenac sodium in a canine urate-induced gonarthritis. J. Health Sci., 47(5): 464-467.
28. Kakoei, S., Baghaei, F., Dabiri, S., Parirokh, M. and Kakooei, S. (2010) A comparative in vivo study of tissue reactions to four suturing materials. Iran. Endod. J., 5(2): 69-73.
PMid:23130030 PMCid:PMC3487505
29. Filho, N., Matsumoto, A., Batista, A., Lopes, C., Ges, S. and Consolaro, A. (2002) Comparative study of tissue response to polyglecaprone 25, polyglactin 910 and polytetrafluorethylene suture materials in rats. Braz. Dent. J., 13(2): 86-91.
30. Tidball, J. and Wehling-Henricks, M. (2007) Macrophages promote muscle membrane repair and muscle fiber growth and regeneration during modified muscle loading in mice in vivo. J. Physiol., 578: 327-336.
PMid:17038433 PMCid:PMC2075127
31. Fortes, M. and Sadi, M. (1996) An experimental comparative study with absorbable sutures in bladder surgery. Rev. Col. Bras. Cir., 23: 83.
32. Andrade, M., Weissman, R. and Reis, S. (2006) Tissue reaction and surface morphology of absorbable sutures after in vivo exposure. J. Mater. Sci-Mater. M., 17: 949-961.
33. Gabrielli, F., Potenza, C., Puddu, P., Sera, F., Masini, C. and Abeni, D. (2001) Suture materials and other factors associated with tissue reactivity, infection and wound dehiscence among plastic surgery outpatients. Plast. Reconstr. Surg., 107: 38-45.
34. Yaltirik, M., Dedeoglu, K., Bilgic, B., Koray, M., Ersev, H. and Issever, H. (2003) Comparison of four different suture materials in soft tissues of rats. Oral Dis., 9: 284-290.
35. Selvig, K., Biagiotti, G., Leknes, K. and Wikesjo, U. (1998) Oral tissue reactions to suture materials. Int. J. Periodont. Rest., 18(5): 475-487.
36. zaka, ., Arikan, F., Snmez, Ş., Veral, A. and Kendirci, S. (2010) Evaluation of the tissue reaction of five different suture materials in rabbit palatal mucosa. E Dişhek Fak Derg., 31: 29-37.
37. Merly, F., Lescaudron, L., Rouaud, T., Crossin, F. and Gardahaut. M. (1999) Macrophages enhance muscle satellite cell proliferation and delay their differentiation. Muscle Nerve, 22: 724-732.<724::AID-MUS9>3.0.CO;2-O
38. Hurty, C., Brazik, D., Law, J., Sakamoto, K. and Lewbart, G. (2002) Evaluation of the issue reactions in the skin and body wall of koi (Cyprinuscarplo) to five suture materials. Vet. Rec., 151: 324-328.
39. Kim, J., Shin, S., Herr, Y., Park, J., Kwon, Y. and Chung, J. (2011) Tissue reactions to suture materials in the oral mucosa of beagle dogs. J. Periodontal Implant Sci., 41: 185-191.
PMid:21954423 PMCid:PMC3175498
40. Altman, G., Diaz, F., Jakuba, C., Calabro, T., Horan, R., Chen, J., Lu, H., Richmond, J. and Kaplan, D. (2003) Silk-based biomaterials. Biomaterials, 24: 401-410.
41. Setzen, G. and Williams, E. (1997) Tissue response to suture materials implanted subcutaneously in a rabbit model. Plast. Reconstr. Surg., 100: 1788-1795.
42. Leknes, K., Rynstrand, L. and Selvig, K. (2005) Human gingival tissue reactions to silk and expanded polytetrafluoroethylene sutures. J. Periodontol., 76(1): 34-42.
43. Kudur, M., Pai, S., Sripathi, H. and Prabhu, S. (2009) Sutures and suturing techniques in skin closure. Indian J. Dermatol. Vet., 75(4): 425-434.
44. Outlaw, K., Vela, A. and O'Leary, J. (1998) Breaking strength and diameter of absorbable sutures after in vivo exposure in the rat. Am. Surg., 64: 348-354.
45. Otten, J., Wiedmann-Al-Ahmad, M., Jahnke, H. and Pelz, K. (2005) Bacterial colonization on different suture materials: A potential risk for intraoral dentoalveolar surgery. J. Biomed. Mater. Res. B., 74(1): 627-635.
46. Banche, G., Roana, J. and Mandras, N. (2007) Microbial adherence on various intraoral suture materials in patients undergoing dental surgery. J. Oral Maxil. Surg., 65(8): 1502-1507.
47. Yilmaz, N., İnal, S., Muğlali, M., Gven, T. and Baş, B. (2010) Effects of polyglecaprone 25, Silk and Gut suture materials on oral mucosa wound healing in diabetic rats: An evaluation of nitric oxide dynamics. Med. Oral Patol. Oral., 15(2): e526-e530.
48. Javed, F., Al-Askar, M., Almas, K., Romanos, G. and Al-Hezaimi, K. (2012) Tissue reactions to various suturematerials used in oral surgical interventions. ISRN Dent., 31: 1-6.
PMid:22645688 PMCid:PMC3356909
49. Abi Rached, R., Toledo, B. and Okamato, T. (1991) Reaction of the human gingival tissue to different suture materials used in periodontal surgery. Braz. Dent. J., 2: 103-106.
50. De Nardo, G., Brown, N., Trenka-Benthin, S. and Marretta, S. (1996) Comparison of seven different suture materials in the feline oral cavity. J. Am. Anim. Hosp. Assoc., 32: 164-172.
51. Parirokh, M., Asgary, S., Eghbal, M., Stowe, S. and Kakoei, S. (2004) A scanning electron microscope study of plaque accumulation on silk and PVDF suture materials in oral mucosa. Int. Endod. J., 37: 776-781.
52. Pi-eros-Fernandez, A., Salopek, L., Rodeheaver, P., Drake, D., Edlich, R. and Rodeheaver, G. (2006) A revolutionary advance in skin closure compared to current methods. J. Long-Term Eff. Med., 16: 19-27.
53. Tuttle, A., Law, J., Harms, C., Lewbart, G. and Harvey, S. (2006) Evaluation of the gross and histologic reactions to five commonly used suture materials in the skin of the African clawed frog (Xenopuslaevis). J. Am. Assoc. Lab. Anim., 45: 22-26.
54. Leknes, K., Selvig, K,.Be, O. and Wikesjo, U. (2005) Tissue reactions to sutures in the presence and absence of anti-infective therapy. J. Clin. Periodontol., 32(2): 130-138.
55. Ribeiro, C., Jnior, S., Neto, S. and Vasconcelos, B. (2005) Clinical and histopathological study of tissue reactivity to monofilament suture materials: Nylon and Poliglecaprone 25 in rats. Acta. Cir. Bras., 20: 284-291.
56. Silverstein, L. and Kurtzman, G. (2005) A review of dental suturing for optimal soft-tissue management. Comp. Cont. Educ. Dent., 26(3): 163-209.
57. Thompson, W., Harvey, J., Kazmi, M. and Stout, A. (1991) Fibrinolysis and angiogenesis in wound healing. J. Pathol., 165: 311-318.
58. Bellenger, C. (1982) Sutures. Part I. The purpose of sutures and available suture materials. Comp. Cont. Educ. Pract., 4: 507-518.
59. Xu, J., Pollock, C. and Kajander, K. (1996) Chromic gut suture reduces calcitonin-gene-related peptide and substance P levels in the spinal cord following chronic constriction injury in the rat. Pain, 64: 503-509.
60. La Bagnara, J. (1995) A review of absorbable suture materials in head and neck surgery and introduction of monocryl: A new absorbable suture. Ear Nose Throat J., 74: 409.
61. Coker, C. (2009) An evidence based approach to soft tissue approximation: A review of suture selection for optimal skin closure. Available from: Last accessed on 05-09-2014.
62. Bernis-Filho, W., Wouters, F., Wouters, A., Bernis, V., Lopes, L. and Andreollo, N. (2013) Comparative study of cotton, polyglactin and polyglecaprone sutures in intestinal anastomoses in dogs. ABCD Arq. Bras. Cir. Dig., 26(1): 18-26.
63. Anderson, J. (1993) Mechanisms of inflammation and infection with implanted devices. Cardiovasc. Pathol., 2: 33S-41S.
64. Farid, S., Beniamino, P., Danielle, L. and Khalid, A. (2008) An innovative method to evaluate the suture compliance in sealing the surgical wound lips. Int. J. Med. Sci., 5(6): 354-360.
65. Saleh, F., Palmieri, B., Lodi, D. and Al-Sebeih, K. (2008) An innovative method to evaluate the suture compliance in sealing the surgical wound lips. Int. J. Med. Sci., 5: 354-360.
PMid:19015745 PMCid:PMC2583338
66. Faulkner, B., Tribble, C., Thacker, J., Rodeheaver, G. and Edlich, R. (1996) Knot performance of polypropylene sutures. J. Biomed. Mater. Res., 33: 187-192.<187::AID-JBM8>3.0.CO;2-M
67. Mulon, P., Zhim, F., Yahia, L. and Desrochers, A. (2010) The effect of six knotting methods on the biomechanical properties of three large diameter absorbable suturematerials. Vet. Surg., 39: 561-565.
68. Huber, D., Egger, E. and James, S. (1999) The effect of knotting method on the structural properties of large diameter nonabsorbable monofilament sutures. Vet. Surg., 28: 260-267.
69. Schisterman, E., Perkins, N., Liu, A. and Bondell, H. (2005) Optimal cut-point and its corresponding youden index todiscriminate individuals using pooled blood samples. Epidemiology, 16: 73-81.
70. Szarmach, R., Livingston, J. and Rodeheaver, G. (2002) An innovative surgical suture and needle evaluation and selection program. J. Long-Term Eff. Med., 12(4): 211-229.
71. McFadden, M. (2011) Suture materials and suture selection for use in exotic pet surgical procedures. J. Exot. Pet. Med., 20(3): 173-181.
72. Charbit, Y., Hitzig, C. and Bolla, M. (1999) Comparative study of physical properties of three suture materials: Silk, e-PTFE (Gore-Tex), and PLA/PGA (Vicryl). Biomed. Instrum. Technol., 33: 71-75.
73. Greenberg, J. and Clark, R. (2009) Advances in suture material for obstetric and gynecologic surgery. Rev. Obstet. Gynecol., 2(2): 146-158.
PMid:19826572 PMCid:PMC2760901
74. Ammirati, C. (2002) Advances in wound closure material. In: James, W., editor. Advances in Dermatology. 18th ed. Mosby, St. Louis, MO. p313-338.
75. Bloom, B. and Golberg, D. (2007) Suture material in cosmetic cutaneous surgery. J. Cosmet. Laser Ther., 9(1): 41-45.
76. Roush, J. (2003) Biomaterials and surgical implants. In: Slatter, D., editor. Textbook of Small Animal Surgery. 3rd ed. Saunders, Philadelphia, PA. p141-148.