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R esearch
(Published online:
12-08-2015)
7.
Comparison of follicular dynamics, superovulatory response, and
embryo recovery between estradiol based and conventional
superstimulation protocol in buffaloes (Bubalus bubalis)
-
Narinder Singh, G. S. Dhaliwal, V. S. Malik, D. Dadarwal, M.
Honparkhe, S. Singhal and P. S. Brar
Veterinary World, 8(8): 983-988
doi:
10.14202/vetworld.2015.983-988
Narinder Singh:
Department of Veterinary Gynaecology and Obstetrics, Guru Angad
Dev Veterinary and Animal Sciences University,
Ludhiana,
Punjab, India; narinder2002us@yahoo.com
G. S. Dhaliwal:
Department of Teaching Veterinary Clinical Complex, Guru Angad Dev
Veterinary and Animal Sciences University, Ludhiana, Punjab,
India;
dr.dhaliwal1960@rediffmail.com
V. S. Malik:
Department of Veterinary Gynaecology and Obstetrics, Guru Angad
Dev Veterinary and Animal Sciences University,
Ludhiana,
Punjab, India; vsmalik11@gmail.com
D. Dadarwal:
Large
Animal Clinical Sciences, Western College of Veterinary Medicine,
University of Saskatchewan, Saskatoon, Canada;
dadarwaldinesh@gmail.com
M. Honparkhe:
Department of Veterinary Gynaecology and Obstetrics, Guru Angad
Dev Veterinary and Animal Sciences University,
Ludhiana,
Punjab, India; honparkhem@rediffmail.com
S. Singhal:
Department of Veterinary Gynaecology and Obstetrics, Guru Angad
Dev Veterinary and Animal Sciences University,
Ludhiana,
Punjab, India; drsinghalvet@gmail.com
P. S. Brar: Department of Veterinary
Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal
Sciences University,
Ludhiana,
Punjab, India;
parkashbrar@gmail.com
Received:
06-05-2015, Revised: 10-07-2015, Accepted: 16-07-2015, Published
online: 12-08-2015
Corresponding author:
Narinder Singh, e-mail: narinder2002us@yahoo.com
Citation:
Singh
N, Dhaliwal GS, Malik VS, Dadarwal D, Honparkhe M, Singhal S, Brar
PS (2015) Comparison of follicular dynamics, superovulatory
response and embryo recovery between estradiol based and
conventional superstimulation protocol in buffaloes (Bubalus
bubalis), Veterinary World 8(8): 983-988.
Abstract
Aim: To evaluate the
follicular dynamics, superovulatory response, and embryo recovery
following superstimulatory treatment initiated at estradiol-17β
induced follicular wave emergence and its comparison with
conventional superstimulatory protocol in buffaloes.
Materials and Methods: Six normal cycling pluriparous
buffaloes, lactating, 90-180 days post-partum, and weighing
between 500 and 660 kg were superstimulated twice with a
withdrawal period of 35 days in between two treatments. In
superstimulation protocol-1 (estradiol group) buffaloes were
administered estradiol-17β (2 mg, i.m.) and eazibreed controlled
internal drug release (CIDR) was inserted intravaginally (day=0)
at the random stage of the estrous cycle. On the day 4, buffaloes
were superstimulated using follicle stimulating hormone (FSH) 400
mg, divided into 10 tapering doses given at 12 hourly intervals.
Prostaglandin F2α analogs (PGF2α) was administered at day 7.5 and
day 8, and CIDR was removed with the second PGF2α injection. In
superstimulation protocol - 2 (conventional group) buffaloes were
superstimulated on the 10th day of the estrous cycle with same FSH
dose regimen and similar timings for PGF2α injections. In both
groups, half of the buffaloes were treated with luteinizing
hormone (LH) 25 mg and other half with 100 ug buserelin;
gonadotrophin releasing hormone (GnRH) analog at 12 h after the
end of FSH treatment. All buffaloes in both protocols were
inseminated twice at 12 and 24 h of LH/GnRH treatment. Daily
ultrasonography was performed to record the size and number of
follicles and superovulatory response.
Results: Significantly higher number of small follicles (<8
mm) was present at the time of initiation of superstimulatory
treatment in the estradiol group compared to the conventional
group (12.5±0.80 vs. 7.3±1.21, respectively, p=0.019), however,
the number of ovulatory size follicles (≥8 mm) did not differ
significantly between the respective groups (15.5±1.24 vs.
12.2±1.30; p=0.054). Total embryos and transferable embryos
recovered were non-significantly higher in the estradiol group
compared to the conventional group (5.83±0.86 vs. 4.67±1.16,
p=0.328, and 3.67±0.93 vs. 2.67±0.68, p=0.437, respectively). The
significant higher proportion of transferable embryos were
recovered in buffaloes treated with LH compared to GnRH (73.3% vs.
48.5%; p=0.044).
Conclusion: The average number of ovulatory size follicles
(>8 mm), corpora lutea, and transferable embryos was higher in
buffaloes superstimulated at estradiol-induced follicular wave
compared to the conventional protocol: Further the percentage of
transferable embryos was significantly higher in buffaloes
administered with LH compared to GnRH.
Keywords: buffalo, embryo, estradiol-17β, superstimulation,
ovulation rate.
References
|
1. 19th Livestock Census-2012, All India Report. Ministry of
Agriculture, Department of Animal Husbandry, Dairying and
Fisheries, Krishi Bhawan, New Delhi, India. |
|
|
2. Baruselli, P.S., Ferreira, R.M., Sales, J.N.S., Gimenes,
L.U., Sá Filho, M.F., Martins, C.M., Rodrigues, C.A. and Bó,
G.A. (2011) Timed embryo transfer programs for management of
donor and recipient cattle. Theriogenology, 76: 1583-1593.
http://dx.doi.org/10.1016/j.theriogenology.2011.06.006
PMid:21798580 |
|
|
3. Luo, W., Wang, Y. and Zhang, Y. (2009) Simulation study on
the efficiencies of MOET nucleus breeding schemes applying
marker assisted selection in dairy cattle. Sci. China C Life
Sci., 52: 296-306.
http://dx.doi.org/10.1007/s11427-009-0003-x
PMid:19294355 |
|
|
4. Drost, M. (2007) Advanced reproductive technology in the
water buffalo. Theriogenology, 68: 450-453.
http://dx.doi.org/10.1016/j.theriogenology.2007.04.013
PMid:17481722 |
|
|
|
5. Baruselli, P.S., Soares, J.G., Gimenes, L.U., Monteiro, B.M.,
Olazarri, M.J. and Carvalho, N.A.T. (2013) Control of buffalo
follicular dynamics for artificial insemination,
superovulation and in vitro embryo production. Buffalo Bull.,
32: 160-176. |
|
|
|
6. Misra, A.K. (1996) Studies on improving certain aspects of
embryo biotechnology in the buffalo. Ph. D. Thesis. CSA
University of Agriculture and Technology, Kanpur, India. |
|
|
7. Nasser, L.F., Adams, G.P., Bo, G.A. and Mapletoft, R.J.
(1993) Ovarian superstimulstory response relative to
follicular wave emergence in heifers. Theriogenology, 40:
713-724.
http://dx.doi.org/10.1016/0093-691X(93)90207-L |
|
|
8. Beg, M.A., Sanwal, P.C. and Yadav, M.C. (1997) Ovarian
response and endocrine changes in buffalo superovulated at mid
luteal and late luteal stage of the estrus cycle: A
preliminary report. Theriogenology, 47: 423-432.
http://dx.doi.org/10.1016/S0093-691X(97)00001-0 |
|
|
9. Bó, G.A. and Mapletoft, R.J. (2014) Historical perspectives
and recent research on superovulation in cattle.
Theriogenology, 81: 38-48.
http://dx.doi.org/10.1016/j.theriogenology.2013.09.020
PMid:24274408 |
|
|
10. Carvalho, N.A.T., Baruselli, P.S., Zicarelli, L.,
Madureira, E.H., Visintin, J.A. and D'Occhio, M.J. (2002)
Control of ovulation with a GnRH agonist after
superstimulation of follicular growth in buffalo:
Fertilization and embryo recovery. Theriogenology, 58:
1641-1650.
http://dx.doi.org/10.1016/S0093-691X(02)01057-9 |
|
|
|
11. Honparkhe, M. (2011) Ovulation synchrony and
superovulatory response after synchronization of follicular
wave emergence in buffaloes. Ph. D. Dissertation, Guru Angad
Dev Veterinary and Animal Sciences University, Ludhiana,
Punjab, India. |
|
|
|
12. Robertson, I. and Nelson, R.E. (1998) Certification and
identification of the embryo. In: Stringfellow, D.A. and
Seidel, S.M., editors. Manual of the International Embryo
Transfer Society. IETS, Savoy, IL. p103-134. |
|
|
|
13. Baruselli, P.S., Madureira, E.H., Visintin, J.A. and
Porto-Filho, R. (2000) Failure of oocyte entry into oviduct in
superovulated buffalo. Theriogenology, 53: 491. |
|
|
|
14. Carvalho, N.A.T., Nagasaku, E.M., Vannucci, F.S., Toledo,
L.M., Carvalho, J.B.P., Carvalho, M.I.A., Gimenes, L.U.,
Ayres, H., Martinz, C.C. and Baruselli, P.S. (2007) Use of
different doses of r-bST associated to a protocol for multiple
ovulation and embryo transfer in buffalo (Bubalus bubalis).
Ital. J. Anim. Sci., 6: 652-654. |
|
|
15. Abd-Allah, S.M, Sharma, R.K, Phulia, S.K. and Inderjeet,
S. (2013) Superovulatory response following transvaginal
follicle ablation in murrah buffalo: Effect of FSH or
PMSG+FSH. Theriogenol. Insight, 3(2): 77-84.
http://dx.doi.org/10.5958/j.2277-3371.3.2.005 |
|
|
16. Presicce, G.A. (2007) Reproduction in water buffalo.
Reprod. Domest. Anim., 42(2): 24-32.
http://dx.doi.org/10.1111/j.1439-0531.2007.00907.x
PMid:17688599 |
|
|
17. Azawi, O.I., Ali, A.J. and Noaman, U.T. (2009) A study on
the ovarian follicular dynamic in Iraqi Northern buffaloes.
Trop. Anim. Health Prod., 41: 79-83.
http://dx.doi.org/10.1007/s11250-008-9156-z
PMid:19052905 |
|
|
18. Campanile, G., Baruselli, P.S., Neglia, G., Vecchio, D.,
Gasparrini, B., Gimenes, L.U., Zicarelli, L. and D'Occhio,
M.J. (2010) Ovarian function in the buffalo and implications
for embryo development and assisted reproduction. Anim.
Reprod. Sci., 121: 1-11.
http://dx.doi.org/10.1016/j.anireprosci.2010.03.012
PMid:20430540 |
|
|
19. Lipinski, L.C., Kozicki, L.E., Martinez, J.L., Piasecki
Junior, S.J., Segui, M.S. and Weiss, R.R. (2006).
Superovulação ovariana e produção de embriões em Bubalus
bubalis (Superovulation and embryo production in Bubalus
bubalis). Arch. Vet. Sci., 11: 44-47.
http://dx.doi.org/10.5380/avs.v11i1.5618 |
|
|
|
20. Misra, A.K. and Joshi, B.V. (1991) Relationship between
the synchrony of donor-recipient oestrus in successful
pregnancies of embryo transfer in buffalo. Buffalo J., 7:
71-75. |
|
|
|
21. Agarwal, S.K., Shankar, U. and Yadav, M.C. (1996). The
effect of priming with folltropin in early cycle on
superovulatory response and quality of embryos in buffaloes.
Buffalo J., 12: 207-211. |
|
|
|
22. Patel, D.V., Singh, S.P., Shukla, H.R., Devanand, C.P. and
Kasiraj, R. (2010) Superovulatory response to FSH and embryo
recovery rate in pandharpuri buffaloes (Bubalus bubalis.)
Buffalo Bull., 29: 244-249. |
|
|
|
23. Heleil, B. and El-Deeb, Y. (2010) Superovulatory response
in relation to follicular dynamics and presence of dominant
follicles in Egyptian buffaloes. Adv. Biol. Res., 4(3):
169-174. |
|
|
24. Li, D.R., Qin, C.S., Wei, Y.M., Luc, F.H., Huang, Q.S.,
Jiang, H.S., Shi, D.S. and Shi. Z.D. (2011) Immunisation
against inhibin enhances follicular development, oocyte
maturation and superovulatory response in water buffaloes.
Reprod. Fert. Develop., 23: 788-797.
http://dx.doi.org/10.1071/RD10279
PMid:21791180 |
|
|
25. Misra, A.K., Kasiraj, R., Mutha Rao, M., Rangareddy, N.S.,
Jaiswal, R.S. and Pant, H.C. (1998) Rate of transport and
development of preimplantation embryo in the superovulated
buffalo (Bubalus bubalis). Theriogenology, 50(4): 637-649.
http://dx.doi.org/10.1016/S0093-691X(98)00168-X |
|
|
26. Gasparrini, B. (2002) In-vitro embryo production in
buffalo species: State of the art. Theriogenology, 57:
237-256.
http://dx.doi.org/10.1016/S0093-691X(01)00669-0 |
|
|
|
27. Carvalho, N.A.T., Bombonato, P.P., Angelo, M.D. and
Baruselli, P.S. (2011) Anatomical and functional
characterization of the genital system of female buffaloes
(Bubalus bubalis) and its implications on multiple ovulation
and embryo transfer. Rev. Bras. Reprod. Anim., 35: 95-103. |
|
|
28. Carvalho, N.A.T., Soares, J.G.S., Vannucci, F.S.,
D'angelo, M., Gallupo, A.G., Melo, G.M., Souza, R.J., Nichi,
M., Gimenes, L.U., Sá Filho, M.F., Martins, C.M., Castriccini,
E. and Baruselli, P.S. (2012) In vitro oocyte transport
through the oviduct of buffalo and crossbred beef cows. Braz.
J. Vet. Res. Anim. Sci., 49: 277-284.
http://dx.doi.org/10.11606/issn.1678-4456.v49i4p277-284 |
|
|
|
29. Soares, J.G., Carvalho, N.A.T., Martins Júnior, B., Souza,
D.C., Maio, J.R.G., Sales, J.N.S., Vieira, L.M. and Baruselli,
P.S. (2013) Progesterone treatment during the periovulatory
period decreases embryo production in superovulated buffaloes.
Buffalo Bull., 32: 517-521. |
|
|
|
30. Misra, A.K., Kasiraj, R., Mutha Rao, M., Rangareddy, N.S.
and Pant, H.C. (1999) Embryo transfer technology in the
buffalo: Our experience and future prospects. Bubalus Bubalis,
4: 63-74. |
|
|
|
31. Jiang, H., Wei, Y., Jiang, Q., Wei, J., Wei, Z., Wei, P.,
Xei, B., Huang, H. and Hao, X. (2006) Pregnancies resulted
from transfer of in-vivo embryos derived from nonsurgical
recovery in superovulated buffaloes. Proceeding of 5th Asian
Buffalo Congress. Nanning, China. p53-58. |
|
|
32. Wang, Z.K., Wu, T.G., Yu, D.Q., Wang, Z., Wang, K.H., Lei,
C. and Lui, Z.H. (1994) Effect of LH on superovulation of
Swamp buffalo in China. Theriogenology, 41: 331.
http://dx.doi.org/10.1016/S0093-691X(05)80241-9 |
|
|
|
33. Soares, J.G., Carvalho, N.A.T., Martins Júnior, B., Souza,
D.C., Maio, J.R.G., Sales, J.N.S., Vieira, L.M. and Baruselli,
P.S. (2013) PGF2α treatment during the periovulatory period
increases the number of embryos recovered from superovulated
buffaloes. Buffalo Bull., 32: 522-526. |
|
|
|
34. Misra, A.K., Kasiraj, R., Mutha Rao, M., Ranga Reddy, N.S.
and Joshi, B.V. (1994) Embryo transfer in buffalo in India:
Progress in the last five years. Proceeding of 4th World
Buffalo Congress, Vol. 3. p501-504. |
|
|
35. Techakumphu, M., Sukavong, Y., Intaramongko, S. and
Intaramongkol, J. (2001) The effect of gonadotropin releasing
hormone on superovulation in elite swamp buffalo cows (Bubalus
bubalis). J. Vet. Med. Sci., 63(8): 853-857.
http://dx.doi.org/10.1292/jvms.63.853 |
|
|
|
36. Zicarelli, L., Baruselli, P.S. and Campanile, G. (2000)
Embryo recovery in buffalo with timed ovulation and
insemination subsequent to follicle superstimulation. In:
Proceedings of 14th International Congress of Animal
Reproduction, Estocolmo. Vol. 2. p16-19. |
|
|
37. Qin, G.S., Chen, M.T., Liang, X.W., Zhang, XF., Pang,
C.Y., Wei, S.J., Huang, F.X. and Jiang, H.S. (2010) Effects of
different hormone combinations on superovulation in river
buffaloes. In: Proceedings of 9th World Buffalo Congress,
Buenos Aires, Argentina. p881-886.
PMid:20724798 |
|
