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Research (Published online: 18-12-2015)

10. Molecular identification of Campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being - Sonuwara Begum, M. Sekar, L. Gunaseelan, Monica Gawande, G. Suganya, P. Annal Selva Malar and A.Karthikeyan

Veterinary World, 8(12): 1420-1423

 

 

   doi: 10.14202/vetworld.2015.1420-1423

 

Sonuwara Begum: Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu, India; Sonuwarabegum@yahoo.com

M. Sekar: Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu, India; muthu-sekar@yahoo.com

L. Gunaseelan: Dean, Veterinary College and Research Institute, Namakkal, Tamil Nadu Veterinary and Animal Sciences University,
Chennai, Tamil Nadu, India; lgseelan@yahoo.co.in

Monica Gawande: Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu, India; drmonicagawande@gmail.com

G. Suganya: Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu, India; suganyamvc06@gmail.com

P. Annal Selva Malar: Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu, India; drannalselvamalar@gmail.com

A. Karthikeyan: Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu, India; drkarthikeyanvet@gmail.com

Received: 10-08-2015, Revised: 02-11-2015, Accepted: 09-11-2015, Published online: 18-12-2015

Corresponding author: Sonuwara Begum, e-mail: Sonuwarabegum@yahoo.com


Citation: Begum S, Sekar M, Gunaseelan L, Gawande M, Suganya G, Malar PAS, Karthikeyan A (2015) Molecular identification of Campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being, Veterinary World 8(12): 1420-1423.



Aim: Campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species.The aim of this study was to identify Campylobacter jejuni and Campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (PCR).

Methodology: A total of 104 number of samples comprising cloacal swab from poultry (38), a rectal swab from dogs (40), and calves (26) were collected for the isolation of thermophilic Campylobacters using conventional culture method. PCR was used for identification of mapA gene for C.jejuni and ceuE for C.coli.

Results: The overall presence of Campylobacter was found to be 67(64.42%) from the samples, out of which 6 isolates belongs to C. jejuni species, were 5(18.51%) from chicken and 1(4.17%) from dog was recorded and about 17 isolates belongs to C. coli species were 9(33.33%), 6 (25%), and 1(9.09%) from chicken, dog and calves was recorded.

Conclusion: Results suggested that Campylobacter reservoirs chicken, calves and pet dogs can play a role as the source of infection to human beings and PCR can be an ideal tool for molecular confirmation at the species level.

Keywords: emerging, pathogen, thermophilic Campylobacter, zoonotic. 



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