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R esearch
(Published
online:
18-12-2015)
10.
Molecular identification of
Campylobacter jejuni and coli from chicken, calves and dogs
to determine its potential threat on human being -
Sonuwara Begum, M. Sekar, L. Gunaseelan, Monica Gawande, G.
Suganya, P. Annal Selva Malar and A.Karthikeyan
Veterinary World, 8(12): 1420-1423
doi:
10.14202/vetworld.2015.1420-1423
Sonuwara Begum: Department of Veterinary Public Health and
Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu,
India; Sonuwarabegum@yahoo.com
M.
Sekar: Department of Veterinary Public Health and
Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu,
India; muthu-sekar@yahoo.com
L.
Gunaseelan: Dean, Veterinary College and Research Institute,
Namakkal, Tamil Nadu Veterinary and Animal Sciences University,
Chennai, Tamil Nadu, India; lgseelan@yahoo.co.in
Monica Gawande: Department of Veterinary Public Health and
Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu,
India; drmonicagawande@gmail.com
G.
Suganya: Department of Veterinary Public Health and
Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu,
India; suganyamvc06@gmail.com
P.
Annal Selva Malar: Department of Veterinary Public Health
and Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu,
India; drannalselvamalar@gmail.com
A.
Karthikeyan: Department of Veterinary Public Health and
Epidemiology, Madras Veterinary College, Chennai, Tamil Nadu,
India; drkarthikeyanvet@gmail.com
Received: 10-08-2015, Revised: 02-11-2015,
Accepted: 09-11-2015, Published online: 18-12-2015
Corresponding author:
Sonuwara Begum, e-mail: Sonuwarabegum@yahoo.com
Citation:
Begum S,
Sekar M, Gunaseelan L, Gawande M, Suganya G, Malar PAS,
Karthikeyan A (2015) Molecular identification of
Campylobacter
jejuni
and
coli
from chicken, calves and dogs to
determine its potential threat on human being,
Veterinary World 8(12):
1420-1423.
Abstract
Aim:
Campylobacter
is an emerging zoonotic pathogen and one of the leading cause of
foodborne infection worldwide and it has been isolated from a
variety of animal species.The aim of this study was to identify
Campylobacter jejuni and Campylobacter coli from
dogs, calves, and poultry using polymerase chain reaction (PCR).
Methodology:
A total of 104 number of samples comprising cloacal swab from
poultry (38), a rectal swab from dogs (40), and calves (26) were
collected for the isolation of thermophilic Campylobacters
using conventional culture method. PCR was used for
identification of mapA gene for C.jejuni and ceuE for
C.coli.
Results:
The overall presence of Campylobacter was found to be
67(64.42%) from the samples, out of which 6 isolates belongs to
C. jejuni species, were 5(18.51%) from chicken and
1(4.17%) from dog was recorded and about 17 isolates belongs to
C. coli species were 9(33.33%), 6 (25%), and 1(9.09%)
from chicken, dog and calves was recorded.
Conclusion:
Results suggested that Campylobacter reservoirs chicken,
calves and pet dogs can play a role as the source of infection
to human beings and PCR can be an ideal tool for molecular
confirmation at the species level.
Keywords:
emerging, pathogen, thermophilic Campylobacter, zoonotic.
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