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R esearch
(Published
online:
23-12-2015)
12.
Single nucleotide polymorphism mining and
nucleotide sequence analysis of Mx1 gene in exonic
regions of Japanese quail -
Diwesh Kumar Niraj, Pushpendra Kumar, Chinmoy Mishra, Raj
Narayan, Tarun Kumar Bhattacharya, Kush
Shrivastava, Bharat Bhushan, Ashok Kumar Tiwari, Vishesh Saxena,
Nihar Ranjan Sahoo and Deepak Sharma
Veterinary World, 8(12): 1435-1443
doi:
10.14202/vetworld.2015.1435-1443
Diwesh Kumar Niraj:
Animal Genetics Division, Indian Veterinary Research Institute,
Izatnagar - 243 122, Uttar Pradesh, India; diwesh12@gmail.com
Pushpendra Kumar:
Animal Genetics Division, Indian Veterinary Research Institute,
Izatnagar - 243 122, Uttar Pradesh, India; pushpendra64@gmail.com
Chinmoy Mishra:
Department of Animal Genetics and Breeding, College of
Veterinary Science and Animal Husbandry, Orissa University of
Agriculture and Technology, Bhubaneswar, Odisha, India;
drchinmoymishra@gmail.com
Raj Narayan:
Department of Avian Genetics and Breeding, Central Avian
Research Institute, Izatnagar - 243 122, Uttar Pradesh, India;
rncari.quails@gmail.com
Tarun Kumar Bhattacharya:
Directorate of Poultry Research, Rajendranagar, Hyderabad,
Telangana, India; bhattacharyatk@gmail.com
Kush Shrivastava:
Animal Genetics Division, Indian Veterinary Research Institute,
Izatnagar - 243 122, Uttar Pradesh, India; kush_vet@yahoo.co.in
Bharat Bhushan:
Animal Genetics Division, Indian Veterinary Research Institute,
Izatnagar - 243 122, Uttar Pradesh, India; bhushan.drbharat@gmail.com
Ashok Kumar Tiwari:
Standardization Division, Indian Veterinary Research Institute,
Izatnagar- 243 122, Uttar Pradesh, India; aktiwari63@yahoo.com
Vishesh Saxena:
Directorate of Poultry Research, Rajendranagar, Hyderabad,
Telangana, India; visheshmeeta@gmail.com
Nihar Ranjan Sahoo:
Animal Genetics Division, Indian Veterinary Research Institute,
Izatnagar - 243 122, Uttar Pradesh, India; vet.nihar@gmail.com
Deepak Sharma:
Animal Genetics Division, Indian Veterinary Research Institute,
Izatnagar - 243 122, Uttar Pradesh, India; ds7758@yahoo.co.in
Received: 02-07-2015, Revised: 31-10-2015, Accepted: 08-11-2015,
Published online: 23-12-2015
Corresponding author:
Pushpendra Kumar, e-mail: pushpendra64@gmail.com
Citation:
Niraj DK, Kumar P, Mishra C, Narayan R, Bhattacharya TK,
Shrivastava K, Bhushan B, Tiwari AK, Saxena V, Sahoo NR, Sharma
D (2015) Single nucleotide polymorphism mining and
nucleotide sequence analysis of Mx1 gene in exonic regions of
Japanese quail, Veterinary World 8(12): 1435-1443.
Abstract
Aim:
An attempt has been made to study
the Myxovirus resistant (Mx1)
gene polymorphism in Japanese quail.
Materials and Methods:
In the present, investigation
four fragments viz.
Fragment I of 185 bp (Exon 3 region), Fragment II of 148 bp (Exon
5 region), Fragment III of 161 bp (Exon 7 region), and Fragment
IV of 176 bp (Exon 13 region) of
Mx1
gene were amplified and screened
for polymorphism by polymerase chain reaction-single-strand
conformation polymorphism technique in 170 Japanese quail birds.
Results:
Out of the four fragments, one
fragment (Fragment II) was found to be polymorphic. Remaining
three fragments (Fragment I, III, and IV) were found to be
monomorphic which was confirmed by custom sequencing. Overall
nucleotide sequence analysis of
Mx1
gene of Japanese quail showed 100%
homology with common quail and more than 80% homology with
reported sequence of chicken breeds.
Conclusion:
The
Mx1
gene is mostly conserved in
Japanese quail. There is an urgent need of comprehensive
analysis of other regions of
Mx1
gene along with its possible
association with the traits of economic importance in Japanese
quail.
Keywords:
Japanese quail,
Mx1
gene, nucleotide sequencing,
polymorphism, polymerase chain reaction-single-strand
conformation
polymorphism.
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