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Research (Published online: 11-12-2015)

3. Effect of cholesterol supplementation on cryosurvival of goat spermatozoa - Sunita Behera, Hiron M. Harshan, K. Lekshmi Bhai and K. N. Aravinda Ghosh

Veterinary World, 8(12): 1386-1391



   doi: 10.14202/vetworld.2015.1386-1391


Sunita Behera: Department of Veterinary Gynaecology and Obstetrics, Veterinary College, Bengaluru, Karnataka, India;

Hiron M. Harshan: Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Kerala, India;

K. Lekshmi Bhai: Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Kerala, India;

K. N. Aravinda Ghosh: Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Kerala, India;

Received: 07-06-2015, Revised: 24-10-2015, Accepted: 31-10-2015, Published: 11-12-2015


Corresponding author: Sunita Behera, e-mail:

Citation: Behera S, Harshan HM, Bhai KL, Ghosh KNA (2015) Effect of cholesterol supplementation on cryosurvival of goat spermatozoa, Veterinary World 8(12): 1386-1391.

Aim: Sperm membrane cholesterol influences cryodamage during cryopreservation. The present study was carried out to evaluate the effect of varying cholesterol levels in Tris based extenders on the freezability of sexually healthy Malabari buck semen.

Materials and Methods: A total of 48 ejaculates from two adults healthy sexually healthy Malabari bucks were utilized for the study. The collected and pooled ejaculates were divided into four groups with Group I serving as Control - I, Group II and III were treated with 1 mg and 2 mg of cholesterol-loaded-cyclodextrin (CLC)/120 106 spermatozoa, respectively, and Group IV treated with 1 mg methyl-β-cyclodextrin (MβCD) served as Control - II. Manual freezing was carried out to cryopreserve the treated and control spermatozoa.

Results: Treatment of semen samples with CLC resulted in improved maintenance of sperm motility at pre-freeze and post-thaw stages of cryopreservation without affecting hypo-osmotic swelling response. Treatment of semen with 1 mg of CLC/120 106 spermatozoa was observed to be better than treatment with 2 mg of CLC/120 106 spermatozoa. In general, MβCD treatment was found to result in significantly lower sperm characteristics than those of Control - I and CLC treatment at pre-feeze and post-thaw stages and when incubated up to 4 h.

Conclusion: Cholesterol treatment of sexually healthy Malabari buck semen was found to hold promise for improving cryopreser-vability of spermatozoa.

Keywords: buck, cholesterol, cryopreservation, functional membrane integrity, motility, spermatozoa.

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