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R esearch
(Published
online:
17-12-2015)
5.
Assessment of aflatoxin B1 in livestock feed and feed
ingredients by high-performance thin layer chromatography -
Korrapati Kotinagu, T. Mohanamba and L. Rathna Kumari
Veterinary World, 8(12): 1396-1399
doi:
10.14202/vetworld.2015.1396-1399
Korrapati Kotinagu:
Toxicology and Feed Analysis Laboratory Veterinary Biological
Research Institute, Shanti Nagar, Hyderabad, Telangana, India;
kotinagu@gmail.com
T.
Mohanamba:
Toxicology and Feed Analysis Laboratory Veterinary Biological
Research Institute, Shanti Nagar, Hyderabad, Telangana, India;
tfalvbri@gmail.com
L.
Rathna Kumari:
Toxicology and Feed Analysis Laboratory Veterinary Biological
Research Institute, Shanti Nagar, Hyderabad, Telangana, India;
jddivbri@gmail.com
Received: 16-07-2015, Revised: 16-10-2015, Accepted: 23-10-2015,
Published online: 17-12-2015
Corresponding author:
Korrapati Kotinagu, e-mail: kotinagu@gmail.com
Citation: Kotinagu K, Mohanamba T, Kumari LR (2015)
Assessment of aflatoxin B1 in livestock feed and feed
ingredients by high-performance thin layer chromatography,
Veterinary World 8(12): 1396-1399.
Abstract
Aim:
Detection of aflatoxin B1 in Livestock compound Feed and feed
ingredients by high-performance thin layer chromatography (HPTLC).
Materials and Methods: Chromatography was performed on
HPTLC silica gel 60 F 254, aluminum sheets by CAMAG automatic
TLC sampler 4, with mobile phase condition
chloroform:acetone:water (28:4:0.06). Extraction of aflatoxin B1
from samples was done as per AOAC method and screening and
quantification done by HPTLC Scanner 4 under wavelength 366 nm.
Results: A total of 97 livestock feed (48) and feed
ingredients (49) samples received from different livestock farms
and farmers were analyzed for aflatoxin B1of which 29 samples
were contaminated, constituting 30%. Out of 48 livestock
compound feed samples, aflatoxin B1 could be detected in 16
samples representing 33%, whereas in livestock feed ingredients
out of 49 samples, 13 found positive for aflatoxin B1
representing 24.5%.
Conclusion: HPTLC assures good recovery, precision, and
linearity in the quantitative determination of aflatoxin B1
extracted from Livestock compound feed and feed ingredients. As
more number of feed and feed ingredients are contaminated with
aflatoxin B1 which causes deleterious effects in both animal and
human beings, so there is a need for identifying the source of
contamination, executing control measures, enabling better risk
assessment techniques, and providing economic benefits.
Keywords: aflatoxin B1, animal feed, feed ingredients,
high-performance thin layer chromatography.
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