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Research (Published online: 17-12-2015)

6. Detection of genes mediating beta-lactamase production in isolates of enterobacteria recovered from wild pets in Saudi Arabia - Sabry A. Hassan and Mohammed Y. Shobrak

Veterinary World, 8(12): 1400-1404



   doi: 10.14202/vetworld.2015.1400-1404


Sabry A. Hassan: Department of Biology, Faculty of Science, Taif University 888, Taif, Saudi Arabia; Department of Microbiology, Faculty of Veterinary Medicine, South Valley University 83523, Qena, Egypt;

Mohamed Y. Shobrak: Department of Biology, Faculty of Science, Taif University 888, Taif, Saudi Arabia;

Received: 24-08-2015, Revised: 19-10-2015, Accepted: 25-10-2015, Published online: 17-12-2015


Corresponding author: Sabry A. Hassan, e-mail:

Citation: Hassan SA, Shobrak MY (2015) Detection of genes mediating beta-lactamase production in isolates of enterobacteria recovered from wild pets in Saudi Arabia, Veterinary World 8(12): 1400-1404.

Aim: To determine the genetic basis and types of beta-lactamase encountered among enterobacterial isolates of wild pets from the animal exhibit.
Materials and Methods: A total of 17 beta-lactamase-producing enterobacteria recovered from fecal samples of wild pet animals were analyzed for a selected beta-lactamase gene by polymerase chain reaction.
Results: Molecular analysis identified one or more β-lactamase-encoding genes in 14 enterobacterial isolates as a single or gene combination. The most frequent extended-spectrum β-lactamases types were TEM and CTX-M, and the most common AmpC enzymes were CMY-2 and DHA types.
Conclusions: The study is the first in Saudi Arabia, have established the presence of β-lactamase-encoding genes in the fecal isolates of wild pets.
Keywords: animal exhibit, extended-spectrum β-lactamases/AmpC beta-lactamase, fecal samples, polymerase chain reaction, Saudi Arabia.

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