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Research
(Published
online: 09-02-2015)
1. Comparison of immunochromatographic
diagnostic test with heminested reverse transcriptase polymerase
chain reaction for detection of rabies virus from brain samples of
various species -
Pranoti Sharma, C. K. Singh and Deepti
Narang
Veterinary World, 8(2): 135-138
doi:
10.14202/vetworld.2015.135-138
Pranoti Sharma: Department of
Veterinary Pathology, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana - 141 004, Punjab, India;
pranoti.sharma22@gmail.com
C. K. Singh: Department of Veterinary
Pathology, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana - 141 004, Punjab, India; rabiesck@gmail.com
Deepti Narang: Department of
Veterinary Microbiology, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana - 141 004, Punjab, India; deeptivet@rediffmail.com
Received:
09-09-2014, Revised: 20-12-2014, Accepted: 30-12-2014, Published
online: 09-02-2015
Corresponding author:
Pranoti Sharma, email: pranoti.sharma22@gmail.com
Citation:
Sharma P, Singh CK, Narang D (2015) Comparison of
immunochromatographic diagnostic test with heminested
reverse
transcriptase polymerase chain reaction
for
detection of rabies virus from brain samples of various species,
Veterinary World, 8(2): 135-138.
Abstract
Aim: Detection of rabies is
a cause of serious concern in developing countries, where dearth
of highly equipped laboratories and trained personnel to handle
sophisticated investigations is felt. The availability of a
diagnostic kit, which can be used in the field, is essential for
diagnosis and control programs as well as for epidemiological
surveillance of the prevalence of the disease. This study was
planned to evaluate anigen rabies Ag test kit for its efficacy to
be used for rapid diagnosis of rabies under field conditions. The
test results were compared with hemi-nested reverse transcriptase
polymerase chain reaction and with a gold standard fluorescent
antibody test.
Materials and Methods: A total of 34 brain samples from
different rabies suspected animals including dogs, buffaloes, cow,
horse, and cat were examined in this study.
Results: Sensitivity of the kit was found to be 91.66%,
specificity 100%, and accuracy was 94.11%.
Conclusion: The study implies that the immunochromatographic
diagnostic test kit may be employed for diagnosis of rabies in
field conditions.
Keywords: antigen, heminested, immunochromatographic, rabies
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