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R esearch
(Published online:
23-02-2015)
15.
Effects of β-mercaptoethanol on in vitro
maturation and glutathione level of buffalo oocytes -
Pankaj A.
Patel, Sandhya S. Chaudhary, Gopal Puri, Virendra Kumar Singh and
Arjun B. Odedara
Veterinary World, 8(2): 213-216
doi:
10.14202/vetworld.2015.213-216
Pankaj
A. Patel:
Department of Veterinary Physiology & Biochemistry, College of
Veterinary Sciences & Animal Husbandry, Navsari Agricultural
University, Navsari-396450, Gujarat, India;
gamitpankaj09@gmail.com
Sandhya
S. Chaudhary:
Department of Veterinary Physiology & Biochemistry, College of
Veterinary Sciences & Animal Husbandry, Navsari
Agricultural University, Navsari-396450, Gujarat, India;
sandhyachaudhary6@gmail.com
Gopal
Puri:
Department of Veterinary Physiology & Biochemistry, College of
Veterinary Sciences & Animal Husbandry, Navsari
Agricultural University, Navsari-396450, Gujarat, India;
drgopalpuri@gmail.com
Virendra Kumar Singh:
Department of Veterinary Physiology & Biochemistry, College of
Veterinary Sciences & Animal Husbandry, Navsari
Agricultural University, Navsari-396450, Gujarat, India;
drvksingh1981@gmail.com
Arjun
B. Odedara: Department of Veterinary Physiology &
Biochemistry, College of Veterinary Sciences & Animal Husbandry,
Navsari
Agricultural University, Navsari-396450, Gujarat, India;
arjunodedra81@gmail.com
Received:
20-11-2014, Revised: 25-12-2014, Accepted: 05-01-2015, Published
online: 23-02-2015
Corresponding author:
Sandhya S. Chaudhary, e-mail: sandhyachaudhary6@gmail.com
Citation:
Patel PA, Chaudhary SS, Puri G, Singh VK, Odedara AB (2015)
Effects of β-mercaptoethanol on in vitro maturation and
glutathione level of buffalo oocytes, Veterinary World
8(2):213-216.
Abstract
Aim:
The present study was carried out to evaluate the effect of
supplementation of β-mercaptoethanol (β-ME) on in vitro maturation
rate and glutathione (GSH) level of buffalo oocytes.
Materials and Methods: Oocytes were recovered from buffalo’s
ovaries collected from government approved slaughter house (near
Kamela darwaza, Surat) of Surat Municipal Corporation. The
obtained oocytes were in vitro matured in maturation media
supplemented with 0 μM (117 oocytes), 100 μM (46 oocytes) and 200
μM (42 oocytes) concentration of β-ME. After 24 h of incubation,
maturation rate of oocytes and intra-cellular GSH level were
determined.
Results: The results showed that the presence of β-ME did not
influence (p>0.05) the oocyte maturation rate. However, GSH level
increased significantly (p<0.05) in matured oocytes when
supplemented with 100 μM and 200 μM β-ME (6.19±0.10 and 6.37±0.20
pmol/oocyte) as compared to control media (4.68±0.26 pmol/oocyte).
Conclusion: It was concluded that β-ME may have a potential to
increase the meiotic maturation of in vitro cultured oocytes and
protect it from oxidative damage.
Keywords: buffalo oocytes, in vitro maturation, β-mercaptoethanol,
glutathione.
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