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R esearch
(Published online:
25-02-2015)
20.
Relative efficacy of egg yolk and soya milk-based extenders for
cryopreservation (−196°C) of buffalo semen -
D.V. Chaudhari, A. J.
Dhami, K. K. Hadiya and J. A. Patel
Veterinary World, 8(2): 239-244
doi:
10.14202/vetworld.2015.239-244
D.V.
Chaudhari:
Sabarmati Ashram Gaushala, Ahmedabad, Gujarat, India;
dvchaudhari@sagbidaj.org
A. J.
Dhami:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Anand, Gujarat, India;
ajdhami@aau.in
K. K.
Hadiya:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Anand, Gujarat, India;
kamleshhadiya@yahoo.co.in
J. A.
Patel:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Anand, Gujarat, India; japatel@aau.in
Received:
28-10-2014, Revised: 03-01-2015, Accepted: 12-01-2015, Published
online: 25-02-2015
Corresponding author:
A. J. Dhami, email: ajdhami@aau.in
Citation:
Chaudhari DV,
Dhami AJ, Hadiya KK, Patel JA (2015) Relative efficacy of egg yolk
and soya milk-based extenders for cryopreservation (−196°C) of
buffalo semen, Veterinary World 8(2): 239-244.
Abstract
Aim:
The aim was to compare commercially available soybean
milk-based extenders, viz. Bioxcell ®
and Optixcell® (IMV, France) with standard
Tris-citrate-fructose-egg yolk-glycerol (TFYG) extender for
cryopreservation of buffalo semen.
Materials and Methods: Semen was collected
twice a week in artificial vagina from six sexually mature, 4-6
years old, healthy breeding bulls of Surti buffalo breed. In all
48 qualifying ejaculates (8 per bull) having initial motility >70%
were split into three equal aliquots and were diluted (at 34°C
keeping 100×106 sperm ml−1)
in TFYG, Bioxcell and Optixcell extenders. The French mini straws
filled from each aliquot were gradually cooled to 4-5°C,
equilibrated at 4°C for 4 h and frozen in liquid nitrogen 2 vapor
using programmable biofreezer. Just before freezing
(post-equilibration) and 24 h after frozen storage, the samples
were evaluated for various sperm quality parameters using standard
protocols. Frozen semen straws were thawed in a water bath at 37°C
for 30 s. The post-thaw incubation survival (37°C for 1 h) was
assessed through motility rating at 0, 30 and 60 min of
incubation.
Results: The mean percentages of prefreeze sperms in TFYG,
Bioxcell and Optixcell extenders in terms of progressive motility
(69.48±0.37, 68.02±0.49, 70.94±0.38), viability (79.21±0.39,
77.38±0.48, 81.58±0.38), total abnormalities (7.90±0.14,
8.60±0.16, 7.08±0.15), intact acrosome (89.54± 0.18, 88.58±0.22,
90.52±0.21) and hypoosmotic swelling (HOS) reactivity (67.96±0.32,
65.65±0.42, 70.23±0.37) varied significantly (p<0.05) between
extenders. Similar pattern of significant (p<0.05) variations
between these extenders for post-thaw sperm progressive motility
(47.71±0.79, 44.38±0.85, 49.90±0.90), viability (57.19±0.79,
53.85±0.84, 59.67±0.91), total abnormalities (12.33±0.17,
12.75±0.21, 11.27±0.18), intact acrosome (76.83±0.23, 75.90± 0.27,
78.50±0.25) and HOS reactivity (45.02±0.84, 42.31±0.82,
47.81±0.90) was also observed for TFYG, Bioxcell and Optixcell
extenders. The recently launched improved soybean milk-based
extender Optixcell excelled the older Bioxcell extender and even
standard TFYG in respect of some of the sperm quality parameters.
Conclusion: The advantages of soy lecithin-based bovine semen
extenders over egg yolk regarding sanitary issues are
unquestionable but still egg yolk-based semen extenders are widely
used because of the cost factor and good in vivo fertility
results.
Keywords: buffalo semen, cryopreservation,
extenders, egg yolk, relative efficacy, soybean milk.
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