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Research (Published online: 10-02-2015)

5. Fungal biological control agents for integrated management of Culicoides spp. (Diptera: Ceratopogonidae) of livestock - B. W. Narladkar, P. R. Shivpuje and P. C. Harke

Veterinary World, 8(2): 156-163



   doi: 10.14202/vetworld.2015.156-163



B. W. Narladkar: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University, Parbhani, Maharashtra, India;

P. R. Shivpuje: Department of Agricultural Entomology, Marathwada Agricultural University, Parbhani, Maharashtra, India;

P. C. Harke: Microbiology unit under DBT research Project, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University, Parbhani- Maharashtra, India;


Received: 29-09-2014, Revised: 26-12-2014, Accepted: 02-01-2015, Published online: 10-02-2015


Corresponding author: B. W. Narladkar, email:

Citation: Narladkar BW, Shivpuje PR, Harke PC (2015) Fungal biological control agents for integrated management of Culicoides spp. (Diptera: Ceratopogonidae) of livestock, Veterinary World 8(2):156-163.

Aim: Entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana had wide host range against insects and hence these are being exploited as fungal bio-pesticide on a large scale. Both fungi are proved pesticides against many crop pests and farmers are well acquainted with their use on the field. Thus, research was aimed to explore the potency of these fungal spores against larval and adult Culicoides midges, a pest of livestock.

Materials and Methods: In-vitro testing of both fungal biological control agents was undertaken in Petri dishes against field collected Culicoides larvae, while in plastic beakers against field collected blood-engorged female Culicoides midges. In-vivo testing was undertaken by spraying requisite concentration of fungal spores on the drainage channel against larvae and resting sites of adult Culicoides midges in the cattle shed. Lethal concentration 50 (LC50) values and regression equations were drawn by following probit analysis using SPSS statistical computerized program.

Results: The results of this study revealed LC50 values of 2692 mg and 3837 mg (108 cfu/g) for B. bassiana and M. anisopliae, respectively, against Culicoides spp. larvae. Death of Culicoides larvae due to B. bassiana showed greenish coloration in the middle of the body with head and tail showed intense blackish changes, while infection of M. anisopliae resulted in death of Culicoides larvae with greenish and blackish coloration of body along with total destruction, followed by desquamation of intestinal channel. The death of adult Culicoides midges were caused by both the fungi and after death growth of fungus were very well observed on the dead cadavers proving the efficacy of the fungus.

Conclusion: Preliminary trials with both funguses (M. anisopliae, B. bassiana) showed encouraging results against larvae and adults of Culicoides spp. Hence, it was ascertained that, these two fungal molecules can form a part of biological control and alternative to chemical control and, therefore, can be inducted in integrated management programs.

Keywords: biocontrol agents, Beauveria bassiana, Culicoides spp, integrated pest management, Metarhizium anisopliae.

1. Mishra, S., Kumar, P. and Malik, A. (2011) Adulticidal and larvicidal activity of Beauveria bassiana and Metarhizium anisopliae against house fly, Musca domestica (Diptera: Muscidae), in laboratory and simulated field bioassays. Parasitol. Res., 108(6): 1483-1492.
2. Sharififard, M., Mossadegh, M.S., Vazirianzadeh, B. and Mahmoudabadi, A.Z. (2011) Laboratory evaluation of pathogenecity of entomopathogenic fungi Beauveria bassiana (Bals) and Metarhizium anisopliae (Metech) Sorok to larvae and adults of the housefly, Musca domestica L (Diptera: Muscidae). Asian J. Biol. Sci., 4(2): 128-137.
3. Mwamburi, L.A., Laing, M.D. and Miller, R.M. (2010) Laboratory screening of insecticidal activities of Beauveria bassiana and Paecilomyces lilacinus against larval and adult house fly (Musca domestica L.). Afr. Entomol., 18: 38-46.
4. Lohmeyer, K.H. and Miller, J.A. (2006) Pathogenicity of three formulations of entomopathogenic fungi for control of adult Haematobia irritans (Diptera: Muscidae). J. Econ. Entomol., 99(6): 1943-1947.
5. Mnyone, L.L., Kirby, M.J., Lwetoijera, D.W., Mpingwa, M.W. and Knols, B.G.J. (2009) Infection of the malaria mosquito, Anopheles gambiae, with two species of entomopathogenic fungi: Effects of concentration, co-formulation, exposure time and persistence. Malar J., 8: 309.
PMid:20030834 PMCid:PMC2808315
6. Bukhari, T, Takken, W. and Koenraadt, C.J.M. (2011) Development of Metarhizium anisopliae and Beauveria bassiana formulations for control of malaria mosquito larvae. Parasit. Vector, 4: 23.
PMid:21342492 PMCid:PMC3051916
7. Priyadarshini, T. and Lekeshmanaswamy, M. (2014) Larvicidal effect of fungus Metarhizium anisopliae on Aedes aegypti. Scrutiny Int. Res. J. Health Med. Sci., 1: 27-30. Available from: Accessed on 22-09-2014.
8. Bukhari, T., Middelman, A., Koenraadt, C.J.M., Takken, W. and Knols, B.G.J. (2010) Factors affecting fungus-induced larval mortality in Anopheles gambiae and Anopheles stephensi. Malar. J., 9: 22.
PMid:20085659 PMCid:PMC2817704
9. Howard, A.F.V., N'Guessan, R., Koenraadt, C.J.M, Asidi, A. and Farenhorst, M. (2010) The entomopathogenic fungus Beauveria bassiana reduces instantaneous blood feeding in wild multi-insecticide-resistant Culex quinquefasciatus mosquitoes in Benin, West Africa. Parasit. Vectors, 3: 87.
PMid:20843321 PMCid:PMC2946288
10. Ansari, M.A., Carpenter, S. and Butt, T.M. (2010) Susceptibility of Culicoides biting midges larvae to the entomo-pathogenic fungus, Metarhizium anisopliae: prospects for bluetonge vector control. Acta Trop., 113(1): 1-6.
11. Ansari, M.A., Carpenter, S., Scholte, E.J., Pope, E.C. and Butt, T.M. (2011) Entomopathogenic fungus as a biological control for an important vector of livestock disease: The Culicoides biting midge. PLoS One, 6(1): e16108.
PMid:21264343 PMCid:PMC3018483
12. Busvine, J.R. (1971) A Critical Review of the Techniques for Testing Insecticides. Commonwealth Agricultural, Bureaux, England. p186-187.
13. Narladkar, B.W., Shastri, U.V. and Shivpuje, P.R. (1992) Breeding habits of Culicoides Schultei (Enderlein, 1908) (Diptera: Ceratopogonidae). Indian. J. Anim. Sci., 62: 944-945.
14. Vision, 2020. (1998) In: Marathwada Agricultural University Parbhani Perspective Plan. Published and edited by Perspective Plan Committee MAU, Parbhani, p13-14.
15. Goettel, M.S. and Inglis, G.D. (1997) Fungi: Hyphomycetes. In: Lacey L.A., editor. Manual of Techniques in Insect Pathology. Vol. 5-3. Academic Press, San Diego. p46, 213-248.
16. Ferron, P. (1978) Biological control of insect pests by entomopathogenic fungi. Ann. Rev. Entomol., 23: 409-442.
17. Starnes, R.L., Liu, C.L. and Marrone, P.G. (1993) History, use and future of microbial insecticides. Am. Entomol., 83: 91.
18. Burges, H.D. and Hussey, H.W., editors. (1971) Technical anthology on the use of diseases to control pests. In: Microbial Control of Insects and Mites. Academic Press, London. p861.
19. Flexner, J.L., Lighthart, B. and Croft, B.A. (1986) The effect of microbial pesticides to non-target, beneficial arthropods. Agric. Ecosyst. Environ., 16: 203-254.
20. Scholte, E.J., Knols, B.G., Samson, R.A. and Takken, W. (2004) Entomopathogenic fungi for mosquito control: A review. J. Insect. Sci., 4: 19.
PMid:15861235 PMCid:PMC528879
21. Clark, T.B., Kellen, W.R., Fukuda, T. and Lindegren, J.E. (1968) Field and laboratory studies on the pathogenicity of the fungus Beauveria bassiana to three genera of mosquitoes. J. Invertebr. Pathol., 11(1): 1-7.
22. Strasser, H., Vey, A. and Butt, T.M. (2000) Are any risks in using entomopathogenic fungi for pest control, with particular reference to the bioactive metabolites of Metarhizium, Tolypocladium and Beauveria species? Biocontrol Sci. Technol., 10: 717-735.
23. Hart, M.P. and MacLeod, D.M. (1955) An apparatus for determining the effects of temperature and humidity on germination of fungus spores. Can. J. Bot., 33: 289-292.
24. Roberts, D.W. (1974) Fungal infections of mosquitoes. In: Aubin, A., Belloncik, S., Bourassa, J.P., LaCoursière, E. and Péllissier, M., editors. Le Contrôle Des Moustiques/Mosquito Control. Canada,Presses de l'Université du Québec, Canada. p143-193.
25. Boucias, D.R. and Pendland, J.C. (1998) Entomopathogenic fungi; fungi imperfecti. In: Boucias, D.R., Pendland, J.C. editors. Principles of Insect Pathology. Vol. 10. Kluwer Academic Publishers, Dordrecht.
26. Daoust, R.A., Ward, M.G. and Roberts, D.W. (1982) Effect of formulation on the virulence of Metarhizium anisopliae conidia against mosquito larvae. J. Invertebr. Pathol., 40: 228-236.
27. Lacey, C.M, Lacey, L.A. and Roberts, D.R. (1988) Route of invasion and histopathology of Metarhizium anisopliae in Culex quinquefasciatus. J. Invertebr. Pathol., 52(1): 108-118.
28. Anon. (2006) Biopesticide Registration Action Document Beauveria bassiana HF23 (PC Code 090305) U.S. Environmental Protection Agency Final Draft December 22, 2006. Biopesticides and Pollution Prevention Division, Office of Pesticide Programs United States Environmental Protection Agency. Available from: Accessed on 24-09-2014.
29. EFSA. (2013) Conclusion on the peer review of the pesticide risk assessment of the active substances Beauveria bassiana strains ATCC-74040 and GHA. EFSA J., 11(1): 3031. Available from: Accessed on 27-09-2014.
30. Stolz, I. (1999) The effect of Metarhizium anisopliae (Metsch.) Sorokin (=flavoviride) Gams and Rozsypal var. acridum (Deuteromycotina: Hyphomycetes) on non-target hymenoptera. In: Ph. D Thesis Universität Basel, Schweiz Homburg/Saar, Basel Germany.