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Research
(Published online:
13-02-2015)
7 .
Isolation and in vitro selection of actinomycetes strains
as potential probiotics for aquaculture
- Milagro García Bernal, Ángel
Isidro Campa-Córdova, Pedro Enrique Saucedo, Marlen Casanova
González, Ricardo Medina Marrero and José Manuel Mazón-Suástegui
Veterinary World, 8(2): 170-176
doi:
10.14202/vetworld.2015.170-176
Milagro García Bernal: Department of
Microbiology, Center for the Study of Bioactive Chemicals (CBQ),
Central University "Marta Abreu" of Las Villas. Road to Camajuaní
Km 5½. Santa Clara 54830. Villa Clara. Cuba.;
mrgarcia@uclv.edu.cu
Ángel Isidro Campa-Córdova: Centro de
Investigaciones Biológicas del Noroeste (CIBNOR), Instituto
Politécnico Nacional No. 195, Col. Playa Palo de Santa Rita Sur,
C.P. 23090. La Paz, Baja California Sur, México;
angcamp04@cibnor.mx
Pedro Enrique Saucedo:
Centro de Investigaciones
Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional
No. 195, Col. Playa Palo de Santa Rita Sur, C.P. 23090. La Paz,
Baja California Sur, México;
psaucedo04@cibnor.mx
Marlen Casanova González: Department
of Microbiology, Center for the Study of Bioactive Chemicals (CBQ),
Central University "Marta Abreu" of Las Villas. Road to Camajuaní
Km 5½. Santa Clara 54830. Villa Clara. Cuba.;
marlencg@uclv.edu.cu
Ricardo Medina Marrero: Department of
Microbiology, Center for the Study of Bioactive Chemicals (CBQ),
Central University "Marta Abreu" of Las Villas. Road to Camajuaní
Km 5½. Santa Clara 54830. Villa Clara. Cuba.;
rpmedina@uclv.edu.cu
José Manuel Mazón-Suástegui: Centro de
Investigaciones Biológicas del Noroeste (CIBNOR), Instituto
Politécnico Nacional No. 195, Col. Playa Palo de Santa Rita Sur,
C.P. 23090. La Paz, Baja California Sur, México;
jmazon04@cibnor.mx
Received:
01-10-2014,
Revised:
25-12-2014, Accepted:
03-01-2015,
Published online: 13-02-2015
Corresponding author:
José Manuel
Mazón-Suástegui, email: jmazon04@cibnor.mx
Citation:
Garcia-Bernal
M, Campa-Córdova AI, Saucedo PE, Casanova-Gonzalez M,
Medina-Marrero R, Mazón-Suástegui JM (2015) Isolation and in vitro
selection of actinomycetes strains as potential probiotics for
aquaculture, Veterinary World 8(2);170-176.
Abstract
Aim: This study was designed to describe a
series of in vitro tests that may aid the discovery of
probiotic strains from actinomycetes.
Materials and Methods: Actinomycetes were isolated from marine
sediments using four different isolation media, followed by
antimicrobial activity and toxicity assessment by the agar
diffusion method and the hemolysis of human blood cells,
respectively. Extracellular enzymatic production was monitored by
the hydrolysis of proteins, lipids and carbohydrates. Tolerance to
different pH values and salt concentrations was also determined,
followed by hydrophobicity analysis and genetic identification of
the most promising strains.
Results: Five out of 31 isolated strains showed antimicrobial
activity against three Vibrio species. Three non-hemolytic
strains (N7, RL8 and V4) among these active isolates yielded
positive results in hydrophobicity tests and exhibited good growth
at salt concentrations ranging from 0% to 10%, except strain RL8,
which required a salt concentration >0.6%. Although these strains
did not grow at pH<3, they showed different enzymatic activities.
Phylogenetic analysis revealed that strains N7 and V4 have more
than 99% identity with several Streptomyces species,
whereas the closest matches to strain RL8 are Streptomyces
panacagri and Streptomyces flocculus, with 98% and
98.2% similarity, respectively.
Conclusion: Three actinomycetes strains showing probiotic-like
properties were discovered using several in vitro tests
that can be easily implemented in different institutions around
the world.
Keywords: aquaculture, marine actinomycetes, probiotics,
Vibrio.
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