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distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 02-01-2015)
1.
Prevalence of Campylobacter species in
milk and milk products, their virulence gene profile and
antibiogram -
Shivani Modi, M. N. Brahmbhatt, Y. A. Chatur
and J. B. Nayak
Veterinary World, 8(1): 1-8
doi:
10.14202/vetworld.2015.1-8
Shivani Modi:
Department of Veterinary Public Health and Epidemiology, College
of Veterinary Science & Animal Husbandry, Anand Agricultural
University, Anand, Gujarat, India;
drshivani30@gmail.com
M.
N. Brahmbhatt:
Department of Veterinary Public Health and Epidemiology, College
of Veterinary Science & Animal Husbandry, Anand Agricultural
University, Anand, Gujarat, India;
mnbrahmbhatt2003@yahoo.com
Y.
A. Chatur:
Department of Veterinary Public Health and Epidemiology, College
of Veterinary Science & Animal Husbandry, Anand Agricultural
University, Anand, Gujarat, India;
dr.yogesh223@gmail.com
J.
B. Nayak: Department of Veterinary Public Health and
Epidemiology, College of Veterinary Science & Animal Husbandry,
Anand Agricultural University, Anand, Gujarat, India;
jbn_anand@yahoo.com
Received: 05-08-2014, Revised: 22-11-2014, Accepted: 29-11-2014,
Published: 02-01-2015
Corresponding author:
Shivani Modi, e-mail: drshivani30@gmail.com
Abstract
Aim:
During the last decades, number of food poisoning cases due to
Campylobacter occurred, immensely. After poultry, raw milk
acts as a second main source of Campylobacter. Therefore,
the present study was undertaken to detect the prevalence of
Campylobacters in milk and milk products and to know the
antibiotic sensitivity and virulence gene profile of
Campylobacter spp. in Anand city, Gujarat, India.
Materials and Methods: A total of 240 samples (85 buffalo milk,
65 cow milk, 30 cheese, 30 ice-cream and 30 paneer) were collected
from the different collection points in Anand city. The samples
were processed by microbiological culture method, and presumptive
isolates were further confirmed by genus and species-specific
polymerase chain reaction using previously reported primer. The
isolates were further subjected to antibiotic susceptibility assay
and virulence gene detection.
Result:
Campylobacter species were detected in 7 (2.91%) raw
milk samples whereas none of the milk product was positive. All
the isolate identified were Campylobacter jejuni. Most of
the isolates showed resistance against nalidixic acid,
ciprofloxacin, and tetracyclin. All the isolates have three
virulence genes cadF, cdtB and flgR whereas
only one isolate was positive for iamA gene and 6 isolates
were positive for fla gene.
Conclusion: The presence of Campylobacter in raw milk
indicates that raw milk consumption is hazardous for human being
and proper pasteurization of milk and adaptation of hygienic
condition will be necessary to protect the consumer from this
zoonotic pathogen.
Keywords: antibiotic susceptibility, Campylobacter jejuni,
polymerase chain reaction, virulence gene
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