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Research
(Published
online: 17-01-2015)
12. In vitro antibacterial effect of Withania somnifera root
extract on Escherichia coli - Mamta Kumari and R. P.
Gupta
Veterinary World, 8(1): 57-60
doi:
10.14202/vetworld.2015.57-60
Mamta
Kumari:
Department of Veterinary Pathology, Lala Lajpat Rai University of
Veterinary and Animal Sciences, Hisar, Haryana, India;
mamtabijarnia@gmail.com
R. P.
Gupta: Department of Veterinary Pathology, Lala Lajpat Rai
University of Veterinary and Animal Sciences, Hisar, Haryana,
India;
rpgupta2007@yahoo.co.in
Received:
05-09-2014, Revised: 09-12-2014, Accepted: 15-12-2014, Published
online: 17-01-2015
Corresponding author:
Mamta Kumari, email: mamtabijarnia@gmail.com
Abstract
Aim: The aim was to
investigate antibacterial activity of Withania somnifera (Ashwagandha),
an Indian traditional medicinal plant against Escherichia coli
O78, a pathogenic strain.
Materials and Methods: Two-fold serial dilutions of 20%
aqueous W. somnifera root (WSR) extract were inoculated with E.
coli O78 @ 1*107 colony forming units grown in nutrient broth.
Following inoculation, turbidity optical density was measured by
spectrophotometer at 600 nm in all the tubes at 0, 2, 4, 6 and 8 h
of incubation at 37°C.
Result: The results revealed that the maximum inhibition of
bacterial growth was observed at 1:8 dilution of WSR extract. The
highest dilution of the extract that showed inhibited growth of
the test organism when compared with control was 1:16. Therefore,
the minimum inhibitory concentration of aqueous extract of WSR is
1:16.
Conclusion: It is concluded that WSR possessed good
antibacterial activity, confirming the great potential of
bioactive compounds and its rationalizing use in health care.
Keywords: antibacterial, Ashwagandha, Withania somnifera,
root extract.
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