Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916

ISSN (Print): 0972-8988


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License

( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online: 17-01-2015)

12. In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli - Mamta Kumari and R. P. Gupta

Veterinary World, 8(1): 57-60



   doi: 10.14202/vetworld.2015.57-60



Mamta Kumari: Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India;

R. P. Gupta: Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India;


Received: 05-09-2014, Revised: 09-12-2014, Accepted: 15-12-2014, Published online: 17-01-2015


Corresponding author: Mamta Kumari, email:

1. Harikrishnan, B., Subramanian, P. and Subash, S. (2008) Effect of Withania somnifera root powder on the levels of circulatory lipid peroxidation and liver marker enzymes in chronic hyperammonemia. E-J. Chem., 5: : 872-877.
2. Kaur, P, Sharma, M. and Mathur, S. (2003) Effect of 1-oxo-5beta, 6betaepoxy- witha-2-ene-27-ethoxy-olide isolated from the roots of Withania somnifera on stress indices in Wistar rats. J. Altern. Complement. Med., 9: 897-907.
3. Manoharan, S, Ramesh, S., Prathiban, M., Koteeswaran, A., Chandran, N.D.J. and M. R. Reddy, M.R. (2004) Effect of a poly herbal ingredient on day old chick quality by feeding in parent flocks. Internat. J. Poult. Sci., 3: 773-778.
4. Ziauddin, M., Phansalkar, N., Patki, P., Diwanay, S. and Patwardhan, B. (1996) Studies on the immunomodulatory effects of Ashwagandha. J. Ethnopharmcol., 50: 69-76.
5. Davis, L. and Kuttan, G. (2002) Effect of Withania somnifera on CTL activity. J. Exp. Clin. Cancer. Res., 21: 115-118.
6. Gupta, D., Goel, A. and Bhatia, A.K. (2010) Studies of antiviral property of Acacia nilotia. J. Environ. Res. Develop., 5: 141-152.
7. Li, X.S., Wang, G.Q., Du, X.D., Cui, B.A., Zhang, S.M. and Shen, J.Z. (2007) Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens. J. Vet. Sci. 8: 243-247.
PMid:17679770 PMCid:PMC2868130
8. Renu. (2010). Pathological investigation of the diseases affecting gastrointestinal tract of Poultry (Dissertation). Hisar: CCS Haryana Agricultural University, Hisar.
9. WHO. (2001). Antimicrobials in animal feed- a threat to human use. WHO Drug Inf.,ormation. 15: 160-162.
10. Pujari, S. A. and Gandhi, M.B. (2012) Studies on effect of root extracts of Withania somnifera on some clinically isolated bacterial pathogens. J. Environ. Res. Develop., 7: 1032-1035.
11. Tsuchiya, H, Sato, M. and Miyazaki, T. (1996) Comparative study on the antibacterial activity of phytochemical flavonones against methicillin resistant Staphyllococcus aureus. J. Ethanopharmcol., 50: 27-34.
12. Adedapo, A.A., Sofidiya, M.O., Maphosa, V., Moyo, B., Masika, P.J., and Afolayan, A.J. (2008) Anti-inflammatory and analgesic activities of the aqueous extract of Cussonia paniculata stem bark. Rec. Nat. Prod., 2(2): 46-53.
13. El-Boshy, M El-S, M.E., Abdalla, O. M., Risha, A. and Moustafa, F. (2013) Effect of Withania somnifera extracts on some selective biochemical, hematological, and immunological parameters in guinea pigs experimental infected with E. coli. ISRN Vet. Sci., 2013, 1534272013, : 6. pages
14. Jain, P. and Varshney, R. (2011) Antimicrobial activity of aqueous and methanolic extracts of Withania somnifera (Ashwagandha), J. Chem. Pharm. Res., 3(3): 260-263.
15. Owais, M., Sharad, K. S., Shehbaz, A. and Saleemuddin, M. (2005) Antibacterial efficacy of Withania somnifera (Ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis., Phytomedicine, 12(3): 229–-235.
16. Sundaram, S., Dwiwedi, P. and Purwar, S. (2011) In vitro evaluation of antibacterial activities of extracts of Withania somnifera (Ashwagandha) to bacterial pathogens, Asian. J. Biotechnol., 3(1): 194-199.
17. Alam, N., Hossain, M., Mottalib, M.A., Sulaiman, S.A., Gan, S.H., and Khalil, M.I. (2012) Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities. BMC Complement Altern. Med., 12: 175.
PMid:23039061 PMCid:PMC3527235
18. Velu, S. and Baskaran, C. (2012) Phytochemical analysis and in-vitro antimicrobial activity of Withania somnifera (Ashwagandha). J. Nat. Prod. Plant Resour., 2(6): 711-716.