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Research (Published online: 29-01-2015)

20. Detection of Avibacterium paragallinarum by Polymerase chain reaction from outbreaks of Infectious coryza of poultry in Andhra Pradesh - T. M. Nabeel Muhammad and B. Sreedevi

Veterinary World, 8(1): 103-108



   doi: 10.14202/vetworld.2015.103-108



T. M. Nabeel Muhammad: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Sri Venkateswara

Veterinary University, Tirupati, Andhra Pradesh, India;

B. Sreedevi: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India;


Received: 18-09-2014, Revised: 15-12-2014, Accepted: 22-12-2014, Published online: 29-01-2015


Corresponding author: B. Sreedevi, e-mail: BS:

Aim: This study was carried out for the detection of Avibacterium paragallinarum from outbreaks of infectious coryza of poultry

Materials and Methods: The polymerase chain reaction (PCR) was standardized for the diagnosis of infectious coryza by using infectious coryza Killed vaccine, ventri biologicals, Pune as source of DNA of A. paragallinarum. Five outbreaks of infectious coryza from Andhra Pradesh were investigated in the present study. A total of 56 infra orbital sinus swabs and 22 nasal swabs were tested by PCR.

Results: PCR analysis showed 56 positives (71.7%) for infectious coryza out of total 78 samples tested. Of 56 infra orbital sinus swabs tested, 47 were positive (83.9%) and 9 nasal swabs (40.9%) out of 22 tested had given positive results for infectious coryza. Samples collected from birds at acute stage of disease and samples collected before treatment with antibiotics were given better results on PCR.

Conclusion: For preventing the economic losses associated with the disease, an early, accurate and rapid diagnosis is essential. PCR is a rapid and highly sensitive diagnostic technique which can substitute conventional cultural examination.

Keywords: infectious coryza, polymerase chain reaction, poultry

1. Yamamoto, R. (1984) Infectious coryza. In: Hofstad, M.S., Barnes H.J., Calnek, BW., Reid, W.M. and Yoder Jr. H.W. (editors.) Diseases of Poultry. 8th ed. Ames, Iowa: Iowa University Press:, Ames, Iowa. p 178-186.
2. Blackall, P. J. and Soriano, V. E. (2005) Infectious coryza and related bacterial infections. In: Saif, Y. M, and (Eeditors.) Diseases of poultry. Iowa State University Press:, Ames, Iowa. p 789-803.
3. Blackall, P. J. and Yamamoto, R. (1998) Infectious coryza. In: Swayne, D.E. and (editors.). A Laboratory Manual for the Isolation and Identification of Avian Pathogens. 4th ed. American Association of Avian Pathologists, Philadelphia.:, p 29-34.
4. Chen, X., Song, C., Gong, Y. and Blackall, P. J. (1998) Further studies on the use of a polymerase chain reaction test for the diagnosis of infectious coryza. Avian Pathol., 27: 618-624.
5. Sambrook, J. and Russel, D. W. (2001) Molecular Cloning: A Laboratory Manual. Vol. 1, 2, and 3. Cold Spring Harbour Laboratory Press:, New YorkVolume 1, 2 and 3.
6. Chen, X., Miflin, J., K., Zhang, P. and Blackall, P. J. (1996) Development and application of DNA probes and PCR tests for Haemophilus paragallinarum. Avian Dis., 40(2): 398-407.
7. Blackall, P. J., Matsumoto, M. and Yamamoto, R. (1997) Infectious coryza. In: Calnek, B.W. and (Eeditors.) Diseases of Poultry. 10th ed. Ames, Iowa: Iowa State University Press, Ames, Iowa.: p 179-190.
8. Sandoval, V. E., Terzolo, H. R. and Blackall, P. J. (1994) Complicated infectious coryza cases in Argentina. Avian Dis., 38: 672-678.
9. Bragg, R. R., Greyling, J. M. and Verschoor, J. A. (1997) Isolation and identification of NAD-independent bacteria from chickens chickens with symptoms of infectious coryza. Avian Pathol., 26(3): 595-606.
10. Sharma, D. R., Gill, B. S. and Kwatra, M. S. (1987) An outbreak of infectious coryza in chicks in Punjab. Poultry Guide., 24: 39-40.
11. Sobti, D. K., Dhaneswar, N. S., Chaturvedi, V. K. and Mehra, K. N. (2001) Isolation and characterization of Haemophilus paragallinarum and morphoculturally related organisms from cases of infectious coryza in Mahakaushal belt. Indian. Vet. J., 78(11): 987-989.
12. Kaur, J., Sharma, N. S., Gupta, K. and Singh, A. (2004) Epidemiological studies on infectious coryza in chickens in northern India. Indian. J. Anim. Sci., 74: 462-465.
13. Rajurkar, G., Roy, A. and Yadav, M. (2009) Incidence of mixed infection in coryza cases. Vet. World., 2(12): 462-464.
14. Rimler, R. B. (1979) Studies of the pathogenic avian haemophili. Avian Dis., 23(4): 1006-1018.
15. Poernomo, S., Sutarma, S., Rafiee. M. and Blackall, P. J. (2000) Characterization of isolates of Haemophilus paragallinarum from Indonesia. Aust. Vet. J., 78(11): 759-762.
16. Byarugaba, D. K., Minga, U. M., Gwakisa, P. S., Katunguka, R. E., Bisgaard, M., Christensen, H. and Olsen, J. E. (2007) Investigations on the occurrence of Avibacterium paragallinarum infections in Uganda. Avian Dis., 51(2): 534-539.[534:IOTOOA]2.0.CO;2
17. Chukiatsiri, K., Chotinun, S. and Chansiripornchain, N. (2010) An outbreak of Avibacterium paragallinarum serovar B in a Thai layer farm. Thai. Vet. Med. J., 40: 441-444.
18. Chen, X., Chen, Q., Zhang, P., Feng, W. and Blackall, P. J. (1998) Evaluation of a PCR test for the detection of Haemophilus paragallinarum in China. Avian Pathol., 27(3):296-300.
19. Yamamoto, R. (1978) Infectious coryza. In: Hofstad, M.S., Calnek, B.W., Hembolt, C. F, Reid W.M. and Yoder, Jr. H.W. (editors.) Diseases of Poultry. 7th ed. Ames, Iowa: Iowa State University Press:, Ames, Iowa. p 225-232.
20. Takagi, M., Takahashi, T., Hirayama, N., Istiananingsi, Mariana, S., Zarkasie, K., Ogata, M . and Ohta, S. (1991) Survey of infectious coryza of chickens in in Indonesia. J. Vet. Med. Sci., 53(4): 637-642.
21. Nagaoka, K., De Mayo, A., Takagi, M. and Ohta, S. (1994) Characterization of Haemophilus paragallinarum isolated in the Philippines. J. Vet. Med. Sci., 56(5): 1017-1019.
22. Thitisak, W., Janviriyasopak, O., Morris, R. S., Srihakim, S. and Kruedener, R. V. (1988) Causes of death found in an epidemiological study of native chickens in Thai villages. Proceedings of 5th International Symposium on Veterinary Epidemiology and Economics.: p 200-202.