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Research
(Published
online: 29-01-2015)
20.
Detection of Avibacterium
paragallinarum by Polymerase chain reaction from outbreaks of
Infectious coryza of poultry in Andhra Pradesh -
T. M. Nabeel Muhammad and B. Sreedevi
Veterinary World, 8(1): 103-108
doi:
10.14202/vetworld.2015.103-108
T. M.
Nabeel Muhammad:
Department of Veterinary Epidemiology and Preventive Medicine,
College of Veterinary Science, Sri Venkateswara
Veterinary
University, Tirupati, Andhra Pradesh, India; nabeelmaliyakkel@gmail.com
B.
Sreedevi: Department of Veterinary Epidemiology and Preventive
Medicine, College of Veterinary Science, Sri Venkateswara
Veterinary University, Tirupati, Andhra Pradesh, India;
bollinisreedevi@rediffmail.com
Received:
18-09-2014, Revised: 15-12-2014, Accepted: 22-12-2014, Published
online: 29-01-2015
Corresponding author:
B. Sreedevi, e-mail: BS: bollinisreedevi@rediffmail.com
Abstract
Aim:
This study was carried out for the detection of
Avibacterium paragallinarum from outbreaks of infectious
coryza of poultry
Materials and Methods: The polymerase chain reaction (PCR) was
standardized for the diagnosis of infectious coryza by using
infectious coryza Killed vaccine, ventri biologicals, Pune as
source of DNA of A. paragallinarum. Five outbreaks of
infectious coryza from Andhra Pradesh were investigated in the
present study. A total of 56 infra orbital sinus swabs and 22
nasal swabs were tested by PCR.
Results: PCR analysis showed 56 positives (71.7%) for
infectious coryza out of total 78 samples tested. Of 56 infra
orbital sinus swabs tested, 47 were positive (83.9%) and 9 nasal
swabs (40.9%) out of 22 tested had given positive results for
infectious coryza. Samples collected from birds at acute stage of
disease and samples collected before treatment with antibiotics
were given better results on PCR.
Conclusion: For preventing the economic losses associated with
the disease, an early, accurate and rapid diagnosis is essential.
PCR is a rapid and highly sensitive diagnostic technique which can
substitute conventional cultural examination.
Keywords: infectious coryza, polymerase
chain reaction, poultry
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