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Research (Published online: 29-01-2015)

22. Clinico-pathology, diagnosis and management of Cysticercus fasciolaris and Hymenolepis diminuta co-infection in wistar rats - Y. Damodar Singh and Rahul Singh Arya

Veterinary World, 8(1): 116-120

 

 

   doi: 10.14202/vetworld.2015.116-120

 

 

Y. Damodar Singh: Department of Veterinary Pathology, College of Veterinary Sciences & Animal Husbandry, Central Agricultural University,

Selesih, Aizawl - 796014, Mizoram, India; dsyengkhom66@gmail.com

Rahul Singh Arya: Department of Veterinary Pathology, College of Veterinary Sciences & Animal Husbandry, Central Agricultural University,

Selesih, Aizawl - 796014, Mizoram, India; aryarahulsingh@gmail.com

 

Received: 12-10-2014, Revised: 18-12-2014, Accepted: 26-12-2014, Published online: 29-01-2015

 

Corresponding author: Rahul Singh Arya, e-mail: aryarahulsingh@gmail.com



Aim: The present study was undertaken to study the pathology and control of sudden unexplained mortality in wistar rats.

Materials and Methods: This study was conducted in a colony of 25 male wistar rats where there was mortality of nine rats. The dead rats were subjected to thorough post-mortem examination and necropsy samples were processed for hematoxylin and eosin staining for histopathological studies. Faecal samples of live rats were studied for the presence of parasitic eggs. Treatment with anthelmintics was given to manage the mortality and infections.

Results: The investigation revealed a natural co-infection of Cysticercus fasciolaris and Hymenolepis diminuta in wistar rats, which were pathogenic enough to cause mortality. Typical lesions associated with the parasites were found in the dead rats. The mortality and infection were managed with common anthelmintics.

Conclusion: C. fasciolaris and H. diminuta infection can cause mortality in wistar rats even when individually they cause asymptomatic infection. The mortality and infection can be managed with common anthelmintics.

Keywords: anthelmintics, co-infection, Hymenolepis diminuta, Mizoram, Taenia taeniaeformis, wistar rats



1. Jithendran, K.P. and Somvanshi, R. (1998) Experimental infection of mice with Taenia taeniaeformis eggs from cats - Course of infection and pathological studies. Indian J. Exp. Biol., 36(5): 523-535.
PMid:9717470
 
2. Soulsby, E.J.L. (2005) Cestodes. In: Helminths, arthropods and protozoa of domesticated animals, 7th ed, Bailliere Tindall, Elsevier, India.
 
3. Baker, D.G. (2007) Parasites of rats and mice. In: Flynn's parasites of laboratory animals, 2nd ed, Blackwell publishing, Ames.
http://dx.doi.org/10.1002/9780470344552.ch11
 
4. Singla, L.D., Aulakh, G.S., Sharma, R., Juyal, P.D. and Singh, J. (2009) Concurrent infection of Taenia taeniaeformis and Isospora felis in a stray kitten: a case report. Vet. Med., 54(2): 81-83.
 
5. Karim, A.J. (2010) Scanning electron microscopy and histological morphology of Cysticercus fasciolaris which induced fibrosarcomas in laboratory rats. Ann. Microsc., 10: 44-48.
 
6. Kataranovski, M., Zolotarevski, L., Belij, S., Mirkov, I., Stošić, J., Popov, A. and Kataranovski, D. (2010) First record of Calodium hepaticum and Taenia taeniaeformis liver infection in wild Norway rats (Rattus norvegicus) in Serbia. Arch. Biol. Sci., 62(2): 431-440.
http://dx.doi.org/10.2298/ABS1002431K
 
7. Rodríguez-Vivas, R.I., Panti-May, J.A., Parada-López, J., Hernández-Betancourt, S.F. and Ruiz-Pi-a, H.A. (2011) The occurrence of the larval cestode Cysticercus fasciolaris in rodent populations from the Cuxtal ecological reserve, Yucatan, Mexico. J Helminthol., 85(4): 458-461.
http://dx.doi.org/10.1017/S0022149X10000817
PMid:21208511
 
8. Pakdel, N., Naem, S., Rezaei, F. and Chalehchaleh, A.A. (2013) A survey on helminthic infection in mice (Mus musculus) and rats (Rattus norvegicus and Rattus rattus) in Kermanshah, Iran. Vet. Res. Forum., 4(2): 105-109.
 
9. Miyazaki, I. (1991). Helminthic Zoonoses. International Medical Foundation of Japan, Tokyo, p494.
 
10. Ekanayake, S., Warnasuriya, N.D., Samarakoon, P.S., Abewickrama, H., Kuruppuarachchi, N.D. and Dissanaike, A.S. (1999) An unusual 'infection' of a child in Sri Lanka with Taenia taeniaeformis of the cat. Ann Trop. Med. Parasitol., 93(8): 869-873.
http://dx.doi.org/10.1080/00034989957871
PMid:10715681
 
11. Mowlavi, G., Mobedi, I., Mamishi, S., Rezaeian, M., HaghiAshtiani, M.T. and Kashi, M. (2008) Hymenolepis diminuta (Rodolphi, 1819) infection in a child from Iran. Iran. J. Publ. Health, 37(2): 120-122.
 
12. Karuna, T. and Khadanga, S. (2013) A case of Hymenolepis diminuta in a young male from Odisha. Trop Parasitol., 3(2): 145-147.
http://dx.doi.org/10.4103/2229-5070.122145
PMid:24471000 PMCid:PMC3888988
 
13. Kołodziej, P., Rzymowska, J., Stępień-Rukasz, H., Lorencowicz, R., Lucińska, M. and Dzióbek, M. (2014) Analysis of a child infected with Hymenolepis diminuta in Poland. Ann. Agri. Environ. Med., 21(3): 510-511.
http://dx.doi.org/10.5604/12321966.1120592
PMid:25292119
 
14. Tiwari, S., Karuna, T. and Rautaraya, B. (2014) Hymenolepis diminuta infection in a child from a rural area: A rare case report. J. Lab. Physician., 6(1): 58-59.
http://dx.doi.org/10.4103/0974-2727.129096
PMid:24696564 PMCid:PMC3969647
 
15. Makki, M.S., Shahbazi, F., Teimoori, S., Rokni, M.B., Abaei, M.R., Mobedi, I., Hassanpour, G. and Mowlavi, G. (2011) Establishment of Hymenolepis diminuta life cycle to provide parasite mass production. Iran. J. Parasitol., 6(2): 60-63.
PMid:22347289 PMCid:PMC3279872
 
16. Andreassen, J. and Hopkins, C.A. (1980) Immunologically mediated rejection of Hymenolepis diminuta by its normal host, the rat. J. Parasitol., 66(60: 898-903.
 
17. Kohn, D.F. and Barthold, S.W. (1984) Biology and diseases of rats. In: Fox, J.G., Cohn, B.J. and Loew, F.M., editors. Laboratory animal medicine. Academic Press, Orlando, Florida, p91-122.
http://dx.doi.org/10.1016/B978-0-12-263620-2.50010-0
 
18. Hanes, M.A. (1995) Fibrosarcomas in two rats arising from hepatic cysts of Cysticercus fasciolaris. Vet. Pathol., 32(4): 441-444.
http://dx.doi.org/10.1177/030098589503200418
PMid:7483224
 
19. Goswami, R., Singh, S.M., Kataria, M. and Somvanshi, R. (2011) Clinicopathological studies on spontaneous Hymenolepis diminuta infection in wild and laboratory rats. Braz. J. Vet. Pathol., 4(2): 103-111.
 
20. Malsawmtluangi, C. and Tandon, V. (2009) Helminth parasite spectrum in rodent hosts from bamboo growing areas of Mizoram, North-east India. J. Parasit. Dis., 33(1&2): 28–35.
http://dx.doi.org/10.1007/s12639-009-0004-5
PMid:23129885 PMCid:PMC3454135
 
21. Malsawmtluangi, C., Prasad, P.K., Biswal, D.K. and Tandon, V. (2011) Morphological and molecular identification of the metacestode parasitizing the liver of rodent hosts in bamboo growing areas of Mizoram, northeast India. Bioinformation,7(8): 393-399.
http://dx.doi.org/10.6026/97320630007393
PMid:22347781 PMCid:PMC3280439
 
22. Luna, L.G. (1968) Manual of Histologic Staining Methods of the AFIP, 3rd ed., McGraw-Hill, New York.
 
23. Insler, G.D. and Roberts, L.S. (1976) Hymenolepis diminuta: lack of pathogenecity in the healthy rat host. Exp. Parasitol., 39(3): 351-357.
http://dx.doi.org/10.1016/0014-4894(76)90038-2
 
24. Moudgil, A.D., Singla, L.D., Gupta, K., Daundkar, P.S. and Vemu, B. (2014) Histopathological and morphological studies on natural Cysticercus fasciolaris infection in liver of Wistar rats. J. Parasit Dis., doi: 10.1007/s12639-014-0488-5.
http://dx.doi.org/10.1007/s12639-014-0488-5
 
25. Irizarry-Rovira, A.R., Wolf, A. and Bolek, M. (2007) Taenia taeniaeformis induced metastatic hepatic sarcoma in a pet rat (Rattus norvegicus). J. Exotic Pet Med., 16(1): 45-48.
http://dx.doi.org/10.1053/j.jepm.2006.11.008
 
26. McKellar, Q.A. (1989) Drug dosages for small mammals. In Pract, 11: 57-61.
http://dx.doi.org/10.1136/inpract.11.2.57