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Research
(Published
online: 06-01-2015)
5.
Hematobiochemical
alterations and direct blood polymerase chain reaction detection
of Theileria annulata in naturally infected crossbred cows
- Anita Ganguly, Vandna Bhanot, R.
S. Bisla, Indrajit Ganguly, Harpreet Singh and S. S. Chaudhri
Veterinary World, 8(1): 24-28
doi:
10.14202/vetworld.2015.24-28
Anita Ganguly:
Teaching Veterinary Clinical Complex, Lala Lajpat Rai University
of Veterinary & Animal Sciences Regional Centre, Karnal, Haryana,
India;
anitaganguly@gmail.com
Vandna Bhanot:
Disease Investigation Laboratory, Krishi Vigyan Kendra, Ambala,
LUVAS, Haryana, India;
vandna_van@gmail.com
R.
S. Bisla:
Teaching Veterinary Clinical Complex, Lala Lajpat Rai University
of Veterinary & Animal Sciences Regional Centre, Karnal, Haryana,
India;
ranbir_bisla@yahoo.com
Indrajit Ganguly:
Division of Animal Genetics, National Bureau of Animal Genetic
Resources, Karnal, Haryana, India;
drindrajit@gmail.com
Harpreet Singh:
Teaching Veterinary Clinical Complex, Lala Lajpat Rai University
of Veterinary & Animal Sciences Regional Centre, Karnal, Haryana,
India;
hsingh@gmail.com
S.
S. Chaudhri:
Teaching Veterinary Clinical Complex, Lala Lajpat Rai University
of Veterinary & Animal Sciences Regional Centre, Karnal, Haryana,
India;
sumer.chaudhri@llruvas.edu.in
Received: 24-08-2014, Revised: 26-11-2014, Accepted: 02-12-2014,
Published online: 06-01-2015
Corresponding author:
Anita Ganguly, email: anitaganguly@gmail.com
Abstract
Aim:
The aim was to determine hemato-biochemical changes and rapid
diagnosis of Theileria annulata in naturally infected
crossbred cows.
Materials and Methods: Blood samples from lactating crossbred
cows (n=40) between 3 and 7 years of age and showing clinical
signs of tropical theileriosis were collected, with or without
anticoagulant, and analyzed for tropical theileriosis by direct
smear, direct blood polymerase chain reaction (PCR) detection of
merozoite-piroplasm surface antigen (Tams1) gene specific amplicon,
estimation of hematological and biochemical parameters. Healthy
crossbred cows (n=6), examined free from hemoprotozoan infections
were included as control.
Results: The infected crossbred cows revealed significantly
(p<0.001) lower values of total erythrocytic counts (4.46±0.2× 106/μL),
hemoglobin (Hb 6.025±0.39 g%), packed cell volume (17.05±1.1%),
mean corpuscular volume (37.94±1.70 fL) and mean corpuscular Hb
(13.5±0.48 pg; p<0.002) compared with healthy control. The serum
samples of infected cows revealed profound (p<0.05) hyponatremia
(Na 133.21±2.36 mEq/l) and hypocalcemia (Ca 8.39±0.34 mg%).
Infected crossbred cows showed a significant increase (p<0.05) of
mean serum activity of alanine aminotransferase (61.45±13.36 U/L),
aspartate aminotransferase (146.1±20.97 U/L), blood urea nitrogen
(28.26±3.90 mg%), creatinine (1.55±0.13 mg%), direct bilirubin
(0.33±0.04 mg%; p<0.001) and lactate dehydrogenase (3001.32±167.0
U/L; p<001). Blood direct PCR revealed a 721-bp fragment amplified
from the target gene encoding 30-kDa major merozoite surface
antigen of T. annulata using specific primer pairs. This
assay was positive for all the infected animals.
Conclusion: The assessments of hemato-biochemical parameters
in T. annulata infected crossbred cows may be useful in
understanding disease pathogenesis, prognosis and corrective
measures for supportive therapy. Moreover, blood direct PCR can
reliably be used for rapid detection of T. annulata in
conjunction with microscopic examination.
Keywords: biochemical parameter, hematological parameter,
serum biochemistry, Tams1 gene, theileriosis.
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