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Research (Published online: 09-01-2015)

7. Epidemiological study of canine parvovirus infection in and around Bhubaneswar, Odisha, India - Monalisa Behera, S. K. Panda, P. K. Sahoo, A. P. Acharya, R. C. Patra, Sweta Das and S. Pati

Veterinary World, 8(1): 33-37

 

 

   doi: 10.14202/vetworld.2015.33-37

 

 

Monalisa Behera: Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India; drmonalisabehera@gmail.com

S. K. Panda: Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India; drsusen_panda@yahoo.com

P. K. Sahoo: Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India; pksahoo1@hotmail.com

A. P. Acharya: Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India; dradityaaditya@gmail.com

R. C. Patra: Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India; rcpatra@gmail.com

Sweta Das: Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India; swetabt@gmail.com

S. Pati: Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India; soumya.pati08@gmail.com

 

Received: 22-10-2014, Revised: 29-11-2014, Accepted: 03-12-2014, Published: 09-01-2015

 

Corresponding author: Monalisa Behera, email: drmonalisabehera@gmail.com



Aim: An epidemiological study of canine parvovirus infection in dogs in and around Bhubaneswar, Odisha was conducted between December 2012 to March 2013 and prevalence rate was studied on the basis of age, breed, and sex.

Materials and Methods: A total of 71 fecal samples from suspected diarrheic dogs were collected in sterile phosphate buffer saline (10% W/V) and examined by polymerase chain reaction (PCR) for detection of canine parvo virus infection, followed by epidemiological study in relation to age, breed, and sex.

Results: Of 71 samples analyzed, 29 (40.85%) were found to be positive by PCR assay. The infection was higher in Deshi/local breeds (34.48%), followed by German shepherd (17.24%), equal incidences in mixed and Labrador retriever (10.34%), Rottweiler and German spitz showed 6.90% each and finally lower incidences in four breeds (3.45%) such as Dalmatians, Nea politan mastiff, Pug and Great Dane. Age-wise prevalence study revealed the infection being more in the age group of 3-6 months (41.37%), followed by equal incidences of 27.59% in 1-3 months and 6-12 months age group, and a low incidence in age groups above 12 months (3.45%). The incidence was predominantly higher in males (86.21%) than females (13.79%).

Conclusions: The epidemiological analysis revealed that the breed wise prevalence was found to be more in Deshi breeds as compared to others, age groups below 6 months were found to be more prone to parvovirus infection and males were mostly infected.

Keywords: canine parvovirus, epidemiology, polymerase chain reaction.



1. Carmichael, L. (2003) Canine infectious diseases- A personal perspective. Proceedings in the international symposium on "Reunion Mundial de Lideres en la Education Veterinaria" that commemorated the 150th anniversary of veterinary education in the college of veterinary medicine, National autonomous university of Mexico: August 16th 2003, at D.F. Baker Institute for Animal Health, Cornell University, Ithaca, New York, USA.
 
2. Bargujar, J., Ahuja, A., Bihani, D.K., Kataria, N. and Dhuria, D. (2011) Studies on prevalence, clinical manifestations and therapeutic management in dogs suffering from canine parvovirus infection. J. Canine Dev. Res., 7: 9-16.
 
3. Balu, P.A. and Thangaraj, T.M. (1981) Canine viral gastroenteritis a clinical report. Indian J. Vet. Med., 1: 73-77.
 
4. Nandi, S., Kumar, M., Chidri, S. and Chauhan, R.S. (2008) Current status of canine parvo virus infection in dogs in India and its pathogenesis. Indian J. Vet. Pathol., 32(2): 150-157.
 
5. Schunck, B., Kraft, W. and Truyen, U. (1995) A simple touch-down polymerase chain reaction for the detection of canine parvovirus and feline panleukopenia virus in faeces. J. Virol. Methods, 55: 427-433.
http://dx.doi.org/10.1016/0166-0934(95)00069-3
 
6. Buonavoglia, C., Martella, V., Pratelli, A., Tempesta, M., Cavalli, A., Buonavoglia, D., Bozzo, G., Elia, G., Decaro, N. and Carmichael, L.E. (2001) Evidence for evolution of canine parvovirus type-2 in Italy. J. Gen. Virol., 82: 1555-1560.
 
7. Phukan, A., Baishya, B., Deka, D. and Boro, P.K. (2010) Prevalence of canine parvovirus infection in Assam. Indian Vet. J., 972-974.
 
8. Srinivas, V.M.V., Mukhopadhyay, H.K., Antony, P.X. and Pillai, R.M (2013) Molecular epidemiology of canine parvovirus in Southern India. Vet. World, 6(10): 744-749.
http://dx.doi.org/10.14202/vetworld.2013.744-749
 
9. Phukan, A., Sarma, D.K., Deka, D. and Boro, P. (2005) Standardization of ELISA for detection of canine parvovirus infection. Indian Vet. J., 82: 355-356.
 
10. Singh, D., Verma, A. K., Kumar, A., Srivastava, M., Singh, S. K., Tripathi, A. K., Srivastava, A. and Ahmed, I. (2013) Detection of canine parvo virus by polymerase chain reaction assay and its prevalence in dogs in and around Mathura, Uttar Pradesh, India. Am J. Biochem. Mol. Biol., 3(2): 264-270.
http://dx.doi.org/10.3923/ajbmb.2013.264.270
 
11. Joshi, D.V., Singh, S.P., Rao, V.D.P. and Patel, B.J. (2000) Diagnosis of canine parvovirus infection by counter immunoelectrophoresis. Indian Vet. J., 77: 899-900.
 
12. Tajpara, M.M., Jhala, M.K., Rank, D.N. and Joshi, C.G. (2009) Incidence of canine parvovirus in diarrhoeic dogs by polymerase chain reaction. Indian Vet. J., 86: 238-241.
 
13. Archana, Shukla, P.C., Gupta, D.K. and Kumar, B. (2009) Epidemiology on canine parvovirus infection. Indian J. Vet. Res., 18(2): 42-44.
 
14. Sellon, R.K. (2005) Canine virus disease. In: Ettinger, S.J. and Feldman, E.C., editors. Veterinary Internal Medicine. 6th ed. Elsevier Saunders, St. Louis, Missouri. p646-652.
 
15. McCaw, D.L. and Hoskins, J.D. (2006) Canine viral enteritis. In: Greene C.E., editor. Infectious Diseases of the Dog and Cat. 3rd ed. Saunders Elsevier, St Louis, USA. p63-70.
 
16. Morais, M.P. and Costa, P.R. (2007) Parvoviridae. In: Flores, E.F., editor. Virologia Veterinária. Ed da UFSM, Santa Maria. p388-392.
 
17. Mohanraj, J., Mukhopadhyay, H.K., Thanislass, J., Antony, P.X. and Pillai, R.M. (2010) Isolation, molecular characterization and phylogenetic analysis of canine parvovirus. Infect, Genet. Evol., 10: 1237-1241.
http://dx.doi.org/10.1016/j.meegid.2010.08.005
PMid:20723622
 
18. Xu, J., Guo, H.C., Wei, Y.Q., Shu, L., Wang, J., Li, J.S., Cao, S.Z. and Sun, S.Q. (2013) Phylogenetic analysis of canine parvovirus isolates from Sichuan and Gansu Provinces of China in 2011. Transbound Emerg. Dis.
 
19. Parthiban, S., Mukhopadhyay, H.K., Panneer, D., Antony, P.X. and Pillai, R.M. (2011) Isolation and typing of canine parvovirus in CRFK cell line in Puducherry, South India. Indian J. Microbiol., 51(4): 456-460.
http://dx.doi.org/10.1007/s12088-011-0105-6
PMid:23024407 PMCid:PMC3209934
 
20. Deka, D., Phukan, A. and Sarma, D.K. (2013) Epidemiology of parvovirus and coronavirus infections in dogs in Assam. Indian Vet. J., 90(9): 49-51.
 
21. Stepita, M.E., Bain, M.J. and Kass, P.H. (2013) Frequency of CPV infection in vaccinated puppies that attended puppy socialization classes. J. Am. Anim. Hosp. Assoc., 49: 95-100.
http://dx.doi.org/10.5326/JAAHA-MS-5825
PMid:23325595
 
22. Carmichael, L.E., Joubert, J.C. and Pollock, R.V.H. (1983) A modified live canine parvovirus vaccine II immune response. Cornell Vet., 73: 13-29.
PMid:6337780
 
23. Gombac, M., Svara, T., Tadic, M. and Pogacnic, M. (2008) Retrospective study of canine parvovirosis in Slovenia. Case report. Slovenia Vet. Res., 45(2): 73-78.
 
24. Thomas, J, Singh, M., Goswami, T.K., Verma, S. and Badasara, S.K. (2014) Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region. Vet. World, 7(11): 929-932.
http://dx.doi.org/10.14202/vetworld.2014.929-932
 
25. Banja, B.K., Sahoo, N., Panda, H.K., Ray, S.K. and Das, P.K. (2002) Epizootiological status of canine viral haemorrhagic gastroenteritis in Bhubaneswar city. Indian Vet. J., 79: 850-851.
 
26. Sanjukta, R., Kumar, M. and Mandakini, R. (2011) Epidemiological study of canine parvovirus. Indian Vet. J., 88(12): 77-79.
 
27. Anderson, N.V. (1980) Veterinary Gastroenterology. 1st ed. Lea and Febiger, Philadelphia.