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Research (Published online: 21-07-2015)

13.  Association of ATP1A1 gene polymorphism with thermotolerance in Tharparkar and Vrindavani cattle - Neeraj Kashyap, Pushpendra Kumar, Bharti Deshmukh, Sandip Bhat, Amit Kumar, Anuj Chauhan, Bharat Bhushan, Gyanendra Singh and Deepak Sharma

Veterinary World, 8(7): 892-897



   doi: 10.14202/vetworld.2015.892-897


Neeraj Kashyap: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;

Pushpendra Kumar: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;

Bharti Deshmukh: Department of Animal Genetics and Breeding, Govind Ballabh Pant University of Agriculture and Technology,

Pantnagar, Uttarakhand, India;

Sandip Bhat: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;

Amit Kumar: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;

Anuj Chauhan: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;

Bharat Bhushan: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;

Gyanendra Singh: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;

Deepak Sharma: Dvision of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;


Received: 19-03-2015, Revised: 13-06-2015, Accepted: 20-06-2015, Published Online: 21-07-2015


Corresponding author: Neeraj Kashyap, e-mail:

Citation: Kashyap N, Kumar P, Deshmukh B, Bhat S, Kumar A, Chauhan A, Bhushan B, Singh G, Sharma D (2015) Association of ATP1A1 gene polymorphism with thermo tolerance in Tharparkar and Vrindavani cattle, Veterinary World 8(7): 892-897.

Aim: One of the major biochemical aspects of thermoregulation is equilibrium of ion gradient across biological membranes. Na+/K+-ATPase, a member of P type-ATPase family, is a major contributor to the mechanism that actively controls crossmembrane ion gradient. Thus, we examined ATP1A1 gene that encodes alpha-1 chain of Na+/K+-ATPase, for genetic polymorphisms.

Materials and Methods: A total of 100 Vrindavani (composite cross strain of Hariana x Holstein-Friesian/Brown Swiss/Jersey) and 64 Tharparkar (indigenous) cattle were screened for genetic polymorphism in ATP1A1 gene, using polymerase chain reaction single-strand conformation polymorphism and DNA sequencing. For association studies, rectal temperature (RT) and respiration rate (RR) of all animals were recorded twice daily for 3 seasons.

Results: A SNP (C2789A) was identified in exon 17 of ATP1A1 gene. Three genotypes namely CC, CA, and AA were observed in both, Vrindavani and Tharparkar cattle. The gene frequencies in Tharparkar and Vrindavani for allele A were 0.51 and 0.48, and for allele C were 0.49 and 0.52, respectively, which remained at intermediate range. Association study of genotypes with RT and RR in both cattle population revealed that the animals with genotype CC exhibited significantly lower RT and higher heat tolerance coefficient than CA and AA genotypes.

Conclusion: Differential thermoregulation between different genotypes of ATP1A1 gene indicate that the ATP1A1 gene could be potentially contributing to thermotolerance in both, Tharparkar, an indigenous breed and Vrindavani, a composite crossbred cattle.

Keywords: ATP1A1 gene, cattle, polymorphism, thermotolerance, Tharparkar, Vrindavani

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