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Research (Published online: 26-07-2015)

17 Detection of Helicobacter pylori glmM gene in bovine milk using Nested polymerase chain reaction - Eyman Y. Osman, A. M. S. El-Eragi, Abuobeida M. Musa, Salma B. El-Magboul, Magdi B. A/Rahman and Abdelmounem E. Abdo

Veterinary World, 8(7): 913-917

 

 

   doi: 10.14202/vetworld.2015.913-917

 

Eyman Y. Osman: Department of Pathology and Diagnosis, Veterinary Research Institute, P. O. Box 8067, ALAmart (St. 1), Khartoum, Sudan; eymanyousif@yahoo.com

A. M. S. El-Eragi: Department of Microbiology, College of Medicine, Bisha University, P.O. Box: 1140, Kingdom of Saudi Arabia; eleragi@gmail.com

Abuobeida M. Musa: Department of Health, Maliha Municipality, P. O. Box: 13888, Sharjah, United Arab Emirates; abousenana2000@yahoo.com

Salma B. El-Magboul: Department of Molecular Biology, Veterinary Research Institute, P. O. Box 8067, ALAmart (St.1), Khartoum, Sudan; Salma.elmagboul@yahoo.com

Magdi B. A/Rahman: Department of Pathology and Diagnosis, Veterinary Research Institute. P. O. Box 8067, ALAmart (St.1), Khartoum, Sudan; mbadawi986@gmail.com

Abdelmounem E. Abdo: Department of Gastroenterology, Directorate of the National Centre for Gastrointestinal and liver disease, Ibnsina Hospital, Alamarat (St.17) postal code 122117 P. O. Box: 15004, Khartoum, Sudan; munem2002@hotmail.com

 

Received: 07-03-2015, Revised: 16-06-2015, Accepted: 27-06-2015, Published online: 26-07-2015

 

Corresponding author: Eyman, Y. Osman, e-mail: eymanyousif@yahoo.com


Citation: Osman EY, El-Eragi AMS, Musa AM, El-Magboul SB, A/Rahman MB, Abdo AE (2015) Detection of Helicobacter pylori glmM gene in bovine milk using Nested polymerase chain reaction, Veterinary World 8(7):913-917.



Aim: The aim was to detect the glmM gene of Helicobacter pylori (H. pylori) in cow’s milk from different dairy farms in Khartoum State using Nested polymerase chain reaction (PCR).

Materials and Methods: A total of 50 milk samples were collected from different dairy farms in Khartoum State (13 from Khartoum, 24 Khartoum North, and 13 from Omdurman Provinces).

Results: The generated results showed that 11/50 (22%) were harboring the investigated H. pylori glmM gene in Khartoum State (1/13 [7.7%] Khartoum, 9/24 [37.5%] Khartoum North, and 1/13 [7.7%] Omdurman provinces, respectively).

Conclusion: To the best of our knowledge, this was the first report on the detection of H. pylori glmM gene in cattle milk in Khartoum State. Nonetheless, the high percentages of H. pylori DNA detection in milk opened new avenues toward exploring the risk of human infection with H. pylori through the consumption of raw milk.

Keywords: bovine, Helicobacter pylori, Khartoum, milk, Nested polymerase chain reaction, glmM gene



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