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R esearch
(Published online:
07-07-2015)
3.
Effect of thawing methods on frozen semen quality of yak (Poephagus
grunniens L.) bulls -
Binod Kumar Dutta
Borah, Bharat Chandra Deka, Ranjan Kumar Biswas, Prithiviraj
Chakravarty, Sourabh Deori, Sudip Sinha and Kutubuddin Ahmed
Veterinary World, 8(7): 831-834
doi:
10.14202/vetworld.2015.831-834
Binod
Kumar Dutta Borah:
ICAR-National Research Centre on Yak, Dirang-790101, Arunachal
Pradesh, India;
beekeyvet@gmail.com
Bharat
Chandra Deka:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science, Assam Agricultural
University, Khanapara, Guwahati -781022, Assam, India; bcdeka@gmail.com
Ranjan
Kumar Biswas:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science, Assam Agricultural
University, Khanapara, Guwahati -781022, Assam, India;
rkbiswascvsc@rediffmail.com
Prithiviraj Chakravarty:
ICAR-National Research Centre on Yak, Dirang-790101, Arunachal
Pradesh, India;
drpcicar@gmail.com
Sourabh
Deori:
ICAR-National Research Centre on Yak, Dirang-790101, Arunachal
Pradesh, India;
sourabhd1@rediffmail.com
Sudip
Sinha:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science, Assam Agricultural University,
Khanapara, Guwahati -781022, Assam, India;
sinhasudip58@gmail.com
Kutubuddin Ahmed: Department of Animal Reproduction,
Gynaecology and Obstetrics, College of Veterinary Science, Assam
Agricultural
University, Khanapara, Guwahati -781022, Assam, India;
kahmedj@gmail.com
Received:
28-02-2015, Revised: 01-06-2015, Accepted: 11-06-2015, Published
online: 07-07-2015
Corresponding author:
Sourabh Deori, e-mail: sourabhd1@rediffmail.com
Citation:
Borah BKD, Deka BC,
Biswas RK, Chakravarty P, Deori S, Sinha S, Ahmed K (2015) Effect
of thawing methods on frozen semen quality of yak (Poephagus
grunniens L.) bulls, Veterinary World 8(7): 831-834.
Abstract
Aim:
To evaluate different thawing temperatures and duration on the
post-thaw semen quality of Indian yaks bulls.
Materials and Methods: Semen ejaculates from four different
yak bulls were collected using artificial vagina method and
extended with tris extender containing 6.4% glycerol at 35°C,
cooled gradually from 35°C to 5°C at 1°C/3 min and equilibrated at
4-5°C for 4 h and frozen in French mini straws using a
programmable bio-freezer and finally stored in liquid
nitrogen.Thawing of frozen semen straws was carried out using
three methods i.e., 35°C for 60 s (thawing method I), 37°C for 30
s (thawing method II) and 75°C for 9 s (thawing method III).The
post-thaw semen quality parameters assessed were sperm motility,
percent live sperm, hypo-osmotic swelling test (HOST)-reacted
sperm, acrosomal changes, and alanine aminotransferase (ALT) and
aspartate aminotransferase (AST) activities in the extracellular
media.
Results: The percent sperm motility, total incidence of
acrosomal changes, and extracellular release of AST varied
significantly (p<0.01) between thawing methods but live sperm and
HOST-reacted sperm did not vary significantly between thawing
methods.The percent sperm motility of frozen yak semen for thawing
method III was significantly (p<0.05) higher than that for thawing
methods I and II, the difference between thawing methods I and II
being non-significant. The critical difference test revealed that
the total incidence of acrosomal changes and extracellular release
of AST were significantly (p<0.05) lower when thawing was done
using methods I and II than in method III.
Conclusion: On the basis of the present experiment, we can
conclude that barring the post-thaw sperm motility, thawing of
frozen yak semen in water either at 35°C for 60 s or 37°C for 30 s
gives better post-thaw semen quality than at 75°C for 09 s.
Keywords: acrosomal changes, post-thaw,
thawing methods, semen quality, yak.
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