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R esearch
(Published online:
20-06-2015)
11.
Hematobiochemical alterations of acute
chlorpyriphos intoxication in indigenous chicken -
Shameem Ara Begum, Tirtha Nath Upadhyaya, Gautam Kumar Baruah,
Taibur Rahman, Debesh Chandra Pathak, Kabita Sarma and Rumi Saikia
Bora
Veterinary World, 8(6): 750-754
doi:
10.14202/vetworld.2015.750-754
Shameem
Ara Begum:
Department of Veterinary Pathology, College of Veterinary Science,
Assam Agricultural University, Khanapara,
Guwahati, India; dr.shameem1@gmail.com
Tirtha
Nath Upadhyaya:
Department of Veterinary Pathology, College of Veterinary Science,
Assam Agricultural University, Khanapara,
Guwahati, India; tnupadhyaya52@gmail.com
Gautam
Kumar Baruah:
Department of Veterinary Pathology, College of Veterinary Science,
Assam Agricultural University, Khanapara,
Guwahati, India; gkbaruah53@gmail.com
Taibur
Rahman: Department
of Veterinary Pathology, College of Veterinary Science, Assam
Agricultural University, Khanapara,
Guwahati, India; dr.taiburrahman@gmail.com
Debesh
Chandra Pathak:
Department of Veterinary Pathology, College of Veterinary Science,
Assam Agricultural University, Khanapara,
Guwahati, India; dcp55@sify.com
Kabita
Sarma: Department
of Anatomy and Histology, College of Veterinary Science, Assam
Agricultural University, Khanapara, Guwahati, India;
kabitasarma09@gmail.com
Rumi
Saikia Bora: Department of Livestock Production and
Management, College of Veterinary Science, Assam Agricultural
University, Khanapara, Guwahati, India;
rumisaikiabora@gmail.com
Received: 20-01-2015, Revised: 10-05-2015, Accepted: 16-05-2015,
Published online: 20-06-2015
Corresponding author:
Shameem Ara Begum,
e-mail: dr.shameem1@gmail.com
Citation:
Begum SA, Upadhyaya
TN, Baruah GK, Rahman T, Pathak DC, Sarma K, Bora RS (2015)
Hematobiochemical’s alterations of acute chlorpyriphos
intoxication in indigenous chicken, Veterinary World 8(6):
750-754.
Abstract
Aim:
The present investigation was undertaken to elaborate
hematobiochemical alterations of acute chlorpyriphos (CPF)
toxicity in indigenous chicken. Since there is no available
literature on the detailed hematobiochemical changes of CPF in
indigenous chicken, hence, the present study was designed to
establish toxicological effect of CPF on blood biochemical
parameters of indigenous chicken which are at a great risk of
exposure to pesticides. These will help physiologist, pathologist,
and poultry scientists for effective production strategy as well
as disease control regime.
Materials and Methods: The birds were
divided into two major Groups I and II. Group I served as control
and Group II was treated with CPF (36 mg/kg). Blood samples were
assayed for hemoglobin (Hb), total erythrocyte count (TEC), total
leukocyte count (TLC), differential leukocyte count, and
biochemical constituents such as alkaline phosphatase (ALP),
aspartate aminotransferase (AST), alanine aminotransferase (ALT),
cholinesterase (CHE), total protein, and uric acid.
Results: Hb, TEC, and TLC levels increased
significantly (p<0.01) in toxin fed birds, whereas, lymphocyte
percent decreased significantly, and heterophil percent increased
significantly. Serum ALP, AST, ALT, and uric acid increased
significantly in CPF treated birds. Decreased serum CHE values
were observed in CPF fed group. The protein level remained almost
same. Uric acid level was found to be increased significantly in
the treated group compared to control.
Conclusion: The results indicated that acute
CPF intoxication produce changes in hematology and biochemical
constituents of the treated birds.
Keywords: acute toxicity, biochemical,
chlorpyriphos, hematology.
References
|
1. Baba, N.A., Raina, R., Verma, P.K., Sultana, M., Prawez, S.
and Nisar, N.A. (2013) Toxic effects of fluoride and
chlorpyrifos on antioxidant parameters in rats: Protective
effects of vitamin E and C. Res. Rep. Fluor, 46(2): 73-79. |
|
|
2. Bharathi, P.A., Reddy, G.A., Reddy, R.A. and Alpharaj, M.
(2011) A study of certain herbs against chlorpyrifos-induced
changes in lipid and protein profile in poultry. Toxicol.
Int., 18(1): 44-46.
http://dx.doi.org/10.4103/0971-6580.75854
PMid:21430920 PMCid:PMC3052583 |
|
|
|
3. Kamon, A.M., Brar, R.S., Sodhi, S., Banga, H.S., Singh, J.
and Nagra, N.S. (2011) Chlorpyrifos chronic toxicity in
broilers and effect of vitamin C. Open Vet. J., 1: 21-27. |
|
|
|
4. Pal, A.K. and Kushwah, H.S. (1998) An assay of brain
acetylecholinesterase activity vis-`a-vis malathion toxicity
in chicken. Indian Vet. Med. J., 22: 233. |
|
|
|
5. Schalm, O.W., Jain, N.C. and Carrol, E.J. (1975) Veterinary
Haematological. 3rd ed. Lea and Febiger, Philadelphia.
p664-667. |
|
|
6. Mohammad, F.K., Al-Badrany, Y.M. and Al-Jobory, M.M. (2008)
Acute toxicity and cholinesterase inhibition in chicks dosed
orally with organophosphate insecticides, Arch. Hyg. Rada
Toxicol., 59: 145-151.
http://dx.doi.org/10.2478/10004-1254-59-2008-1873 |
|
|
|
7. Benjamin, M.M. (1979) Outline of Veterinary Clinical
Pathology. 3rd ed. The Iowa State University Press, Ames, Iowa
USA. |
|
|
|
8. Sastry, G.A. (1983) Veterinary Clinical Pathology. CBS
Publishers and Distrbutors Private Limited, New Delhi. |
|
|
|
9. Malik, G., Dhahiya J.P., Sandeep G. and Mshra S.K. (2002)
Clinico-pathological studies on chlorpyriphos intoxication in
broiler chicken. Proceedings of Indian Association of
Veterinary Pathologists, CSKHPKV, Palampur, Himachal Pradesh,
India.p26-28. |
|
|
10. Akhtar, N., Srivastava, M.K. and Raizada, R.B. (2009)
Assesment of chlorpyrifos toxicity on certain organs in rats.
J. Environ. Biol., 30: 1047-1053.
PMid:20329403 |
|
|
|
11. Tamuli, S.M. (1998) Pathology of experimental paraquat
toxicity in calves, Ph.D. Thesis, Assam Agricultural
University, Khanapara, Guwahat. |
|
|
|
12. Lumeiz, J.T. (1997) Avian clinical biochemistry. In:
Clinical Biochemistry of Domestic Animals. Academic Press, New
York. p857-883. |
|
|
|
13. Kammon, A.M., Brar, R.S., Banga, H.S. and Sodhi, S. (2010)
Patho-biochemical studies on hepatotoxicity and nephrotoxicity
on exposure to chlorpyrifos and imidacloprid in layer
chickens. Vet. Arch., 80: 663-672. |
|
|
14. Banaee, M., Sureda, A., Mirvaghefi, A.R. and Ahmadi, K.
(2011) Effects of diazinon on biochemical parameters of blood
in Rainbow trout (Oncorhynchus mykiss). Pestic. Biochem.
Physiol., 99: 1-6.
http://dx.doi.org/10.1016/j.pestbp.2010.09.001 |
|
