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R esearch
(Published online:
24-06-2015)
15.
Partial characterization of a novel anti-inflammatory protein from
salivary gland extract of Hyalomma anatolicum anatolicum
(Acari: Ixodidae) ticks -
Mayukh Ghosh, Nirmal
Sangwan and Arun K. Sangwan
Veterinary World, 8(6): 772-776
doi:
10.14202/vetworld.2015.772-776
Mayukh Ghosh:
Department of Veterinary Physiology and Biochemistry, College of
Veterinary Sciences, Lala Lajpat Rai University of
Veterinary and Animal Sciences, Hisar, Haryana, India;
ghosh.mayukh87@gmail.com
Nirmal Sangwan:
Department of Veterinary Physiology and Biochemistry, College of
Veterinary Sciences, Lala Lajpat Rai University of
Veterinary and Animal Sciences, Hisar, Haryana, India;
nirmalsangwan@gmail.com
Arun
K. Sangwan: Department of Veterinary Parasitology, College of
Veterinary Sciences, Lala Lajpat Rai University of Veterinary and
Animal Sciences, Hisar, Haryana, India;
sangwan_arun@hotmail.com
Received: 22-01-2015, Revised: 16-05-2015, Accepted: 23-05-2015,
Published online: 24-06-2015
Corresponding author:
Nirmal Sangwan, e-mail: nirmalsangwan@gmail.com
Citation:
Ghosh M, Sangwan N,
Sangwan AK (2015) Partial characterization of a novel
anti-inflammatory protein from salivary gland extract of
Hyalomma anatolicum anatolicum (Acari: Ixodidae) ticks,
Veterinary World 8(6):772-776.
Abstract
Aim:
Hyalomma anatolicum anatolicum ticks transmit
Theileria annulata, causative agent of tropical theileriosis
to cattle and buffaloes causing a major economic loss in terms of
production and mortality in tropical countries. Ticks have evolved
several immune evading strategies to circumvent hosts’ rejection
and achieve engorgement. Successful feeding of ticks relies on a
pharmacy of chemicals located in their complex salivary glands and
secreted saliva. These chemicals in saliva could inhibit host
inflammatory responses through modulating cytokine secretion and
detoxifying reactive oxygen species. Therefore, the present study
was aimed to characterize anti-inflammatory peptides from salivary
gland extract (SGE) of H. a. anatolicum ticks with a view
that this information could be utilized in raising vaccines,
designing synthetic peptides or peptidomimetics which can further
be developed as novel therapeutics.
Materials and Methods: Salivary glands were dissected out from
partially fed adult female H. a. anatolicum ticks and
homogenized under the ice to prepare SGE. Gel filtration
chromatography was performed using Sephadex G-50 column to
fractionate the crude extract. Protein was estimated in each
fraction and analyzed for identification of anti-inflammatory
activity. Sodium dodecyl sulfate - polyacrylamide gel
electrophoresis (SDS-PAGE) was run for further characterization of
protein in desired fractions.
Results: A novel 28 kDa protein was identified in H. a.
anatolicum SGE with pronounced anti-inflammatory activity.
Conclusion: Purification and partial
characterization of H. a. anatolicum SGE by size-exclusion
chromatography and SDSPAGE depicted a 28 kDa protein with
prominent anti-inflammatory activity.
Keywords: anti-inflammatory, Hyalomma anatolicum anatolicum,
size-exclusion chromatography, sodium dodecyl sulfate -
polyacrylamide gel electrophoresis, tropical theileriosis.
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