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R esearch
(Published online:
24-06-2015)
17.
Clinico-pathology, hematology, and biochemistry responses toward
Pasteurella multocida Type B: 2 via oral and subcutaneous
route of infections -
Eric Lim Teik Chung,
Faez Firdaus Jesse Abdullah, Lawan Adamu, Ali Dhiaa Marza, Hayder
Hamzah Ibrahim, Mohd Zamri-Saad, Abdul Wahid Haron, Abdul Aziz
Saharee, Mohd Azmi Mohd Lila, Abdul Rahman Omar, Md Zuki Abu Bakar
and Mohd Jefri Norsidin
Veterinary World, 8(6): 783-792
doi:
10.14202/vetworld.2015.783-792
Eric
Lim Teik Chung:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor,
Malaysia; eric88lim@gmail.com
Faez
Firdaus Jesse Abdullah:
Department of
Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;
Department of Ruminant Disease, Research Centre for Ruminant
Disease, Universiti Putra Malaysia, 43400 Serdang, Selangor,
Malaysia;
jesseariasamy@gmail.com
Lawan
Adamu: Department
of Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;
Department of Veterinary Medicine, Faculty of Veterinary Medicine,
University of Maiduguri, PMB1069, Borno State, Nigeria;
drlawan3758@yahoo.com
Ali
Dhiaa Marza:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor,
Malaysia; Department of Veterinary Internal Medicine, Faculty of
Veterinary Medicine, Al-Qasim Green University, Iraq;
ali.alhashimi471969@yahoo.com
Hayder
Hamzah Ibrahim:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor,
Malaysia; Department of Veterinary Medicine, Technical Institute
Babil, Al Furat Alawast Technical University, Iraq;
altee.hayder@yahoo.com
Mohd
Zamri-Saad:
Department of Veterinary Pathology and Microbiology, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia;
mzamri@upm.edu.my
Abdul
Wahid Haron:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor,
Malaysia; Department of Ruminant Disease, Research Centre for
Ruminant Disease, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia; wahidh@upm.edu.my
Abdul
Aziz Saharee:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor,
Malaysia; abdaziz@upm.edu.my
Mohd
Azmi Mohd Lila:
Department of Veterinary Pathology and Microbiology, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia;
azmi@upm.edu.my
Abdul
Rahman Omar:
Department of Veterinary Pathology and Microbiology, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia;
aro@upm.edu.my
Md Zuki
Abu Bakar:
Department of Preclinical, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;
zuki@upm.edu.my
Mohd
Jefri Norsidin: Department of Veterinary Clinical Studies,
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia;
jaretguli@gmail.com
Received:
04-03-2015, Revised: 20-05-2015, Accepted: 26-05-2015, Published
online: 24-06-2015
Corresponding author:
Faez Firdaus Jesse
Abdullah, e-mail: jesseariasamy@gmail.com
Citation:
Chung ELT, Abdullah
FFJ, Adamu L, Marza AD, Ibrahim H, Zamri-Saad M, Haron AW, Saharee
AA, Lila MAM, Omar AR, Bakar MZA, Norsidin MJ, Clinico-pathology,
hematology and biochemistry responses towards pasteurella
multocida Type B:2 via oral and subcutaneous route of
infections, Veterinary World 8(6):783-792.
Abstract
Background: Pasteurella multocida a Gram-negative
bacterium has been identified as the causative agent of many
economically important diseases in a wide range of hosts.
Hemorrhagic septicemia is a disease caused by P. multocida
serotype B:2 and E:2. The organism causes acute, a highly fatal
septicemic disease with high morbidity and mortality in cattle and
more susceptible in buffaloes. Therefore, the aim of this study
was to investigate the clinical signs, blood parameters, post
mortem and histopathology changes caused by P. multocida
Type B:2 infections initiated through the oral and subcutaneous
routes.
Methods: Nine buffalo heifers were divided equally into 3
treatment groups. Group 1 was inoculated orally with 10 ml of
phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml
of 10 12 colony forming unit of P.
multocida Type B:2 subcutaneously and orally respectively.
Results: There was a significant difference (p<0.05) in
temperature between the subcutaneous and the control group. The
results revealed significant differences (p<0.05) in erythrocytes,
hemoglobin, packed cell volume, leukocytes, monocytes, and A: G
ratio between the subcutaneous and the control group. Furthermore,
there were significant differences (p<0.05) in leukocytes, band
neutrophils, segmented neutrophils, lymphocytes, eosinophils,
basophils, thrombocytes, plasma protein, icterus index, gamma
glutamyl tranferase and A: G ratio between the oral and the
control group. The post mortem lesions of the subcutaneous group
buffaloes showed generalized hyperemia, congestion and hemorrhage
of the immune organs, gastrointestinal tract organs and vital
organs. The oral group buffaloes showed mild lesions in the lung
and liver. Histologically, there were significant differences
(p<0.05) in hemorrhage and congestion; necrosis and degeneration;
inflammatory cells infiltration; and edema in between the groups.
Conclusion: This study was a proof that oral route infection
of P. multocida Type B:2 can be used to stimulate host cell
responses where oral vaccine through feed can be developed in the
near future.
Keywords: buffalo heifers, clinico-pathology,
hematology and biochemistry responses, oral route, Pasteurella
multocida Type B:2, subcutaneous route.
References
1. De Alwis, M.C.L. (1992) Haemorrhagic septicaemia – A
general review. Br. Vet. J., 148: 99-112.
http://dx.doi.org/10.1016/0007-1935(92)90101-6 |
|
|
2. De Alwis, M.C.L. (1999) Haemorrhagic Septicaemia,
Australian Centre for International Agriculture Research (ACIAR)
Monograph 57. ACIAR, Canberra, Australia.
PMid:10543617 |
|
|
|
3. OIE. (2008) Haemorrhagic septicaemia. OIE Terrestrial
Manual. Ch. 2. OIE, United Kingdom. p739-751. |
|
|
4. Zamri-Saad, M. (2013) Haemorrhagic Septicaemia of Cattle &
Buffaloes in Asia. Selangor: Universiti Putra Malaysia Press.
PMCid:PMC3858595 |
|
|
5. Heddleston, K.L., Gallagher, J.E. and Rebers, P.A. (1972)
Fowl cholera: Gel diffusion precipitin test for serotyping
Pasteurella multocida from avian species. Am. Assoc. Avian
Pathol., 14(4): 925-936.
http://dx.doi.org/10.2307/1588773 |
|
|
6. Carter, G.R. (1962) Further observation on typing
Pasteurella multocida by the indirect hemagglutination test.
Can. J. Comp. Med. Vet. Sci., 26: 238-240.
PMid:17649399 PMCid:PMC1583570 |
|
|
7. DeAlwis, M.C.L. (1981) Mortality among cattle and buffaloes
in Sri Lanka due to Haemorrhagic septicaemia. Trop. Anim.
Health Prod., 13: 195-202.
http://dx.doi.org/10.1007/BF02237925 |
|
|
|
8. Farooq, U., Saeed, Z., Khan, M.A., Ali, I. and Qamar, M.F.
(2011) Sero-surveillane of haemorrhagic septicaemia in
buffaloes and cattle in Southern Punjab, Pakistan. Pak. Vet.
J., 31(3): 254-256. |
|
|
9. Thomas, J. (1972) The control of haemorrhagic septicaemia
in west Malaysia. Trop. Anim. Health Prod., 4: 95-101.
http://dx.doi.org/10.1007/BF02359741
PMid:4671395 |
|
|
|
10. Saharee, A.A., Salim, N.B., Rasedee, A. and Jainudeen,
M.R. (1993) Haemorrhagic septicaemia carriers among cattle and
buffalo in Malaysia. Pasteurellosis in Production Animals.
ACIAR Proceeding No. 43. p89-91. |
|
|
|
11. Kahn, C.M. and Line, S. (2005) The Merck Veterinary
Manual. 9th ed. Merial, USA. |
|
|
|
12. Jesse, F.F.A., Khaleel, M.M., Adamu, L., Osman, A.Y.,
Wahid, A., Zamri-saad, M. and Rahman, A. (2013) Polymerase
chain reaction detection of Pasteurella multocida Type B2 in
mice infected with contaminated river water. Am. J. Vet. Res.,
8(3): 146-151. |
|
|
|
13. Ali, O.S., Adamu, L., Abdullah, F.F.J., Ilyasu, Y., Abba,
Y., Hamzah, H.B., Mohd-Azmi, M.L., Haron, A.W.B. and Saad,
M.Z.B. (2014) Alterations in interleukin-1β and interleukin-6
in mibe inoculated through the oral routes using graded doses
of P. Multocida Type B: 2 and its lipopolysaccharide. Am. J.
Vet. Res., 9(4): 177-184. |
|
|
14. Abubakar, M.S. and Zamri-saad, M. (2011)
Clinico-pathological changes in buffalo calves following oral
exposure to Pasteurella multocida B2. Basic Appl. Pathol., 4:
130-135.
http://dx.doi.org/10.1111/j.1755-9294.2011.01113.x |
|
|
|
15. Abubakar, M.S., Zamri-saad, M. and Jasni, S. (2012)
Ultrastructural changes and bacterial localization in buffalo
calves following oral exposure to Pasteurella multocida B: 2.
Pak. Vet. J., 33(1): 101-106. |
|
|
16. Lane, E.P., Kock, N.D., Hill, F.W.G. and Mohan, K. (1992)
An outbreak of haemorrhagic septicaemia in cattle in Zimbabwe.
Trop. Anim. Health Prod., 24: 97-102.
http://dx.doi.org/10.1007/BF02356952
PMid:1305340 |
|
|
17. Rhoades, K.R., Heddleston, K.L. and Rebers, P.A. (1967)
Experimental haemorrhagic septicaemia: Gross and microscopic
lesions resulting from acute infections and from endotoxin
administration. Can. J. Comp. Med., 31: 226-233.
PMid:4227916 PMCid:PMC1494726 |
|
|
|
18. Zamri-Saad, M., Azri, A. and Sheikh-Omar, A.R. (1994)
Experimental respiratory infection of goats with Mycoplasma
argini and Pasteurella haemolytica A: 2. Pertanika J. Trop.
Agric. Sci., 17(3): 239-242. |
|
|
|
19. Sastry, G.A. (2006) Veterinary Clinical Pathology. CBS
Publishers & Distributors, New Delhi. |
|
|
|
20. Weiss, D.J. and Wardrop, K.J. (2010) Schalm's Veterinary
Hematology. 6th ed. Wiley-Blackwell, Iowa. |
|
|
|
21. Jesse, F.F.A., Adamu, L., Osman, A.Y., Wahid, A., Saharee,
A.A., Abdullah, R., Zamri-saad, M. and Zakaria, Z. (2013)
Biochemical and haematological alterations in mice inoculated
with outer membrane protein, lipopolysaccharides and whole
cells of Pasteurella multocida type B2. Am. J. Vet. Res.,
8(3): 152-158. |
|
|
22. Jesse, F.F.A., Osman, A.Y., Adamu, L., Zakaria, Z.,
Abdullah, R., Zamri-saad, M. and Saharee, A.A. (2013)
Haematological and biochemical alterations in calves following
infection with Pasteurella multocida type B: 2, bacterial
lipopolysaccharide and outer membrane protein immunogens.
Asian. J. Anim. Vet. Adv., 8(6): 806-813.
http://dx.doi.org/10.3923/ajava.2013.806.813 |
|
|
|
23. Steven, L.S. (2000) Hematologic changes due to bacterial
infections. In: Schalms's Veterinary Hematology. 5th ed.
Lippincott Williams and Wilkins, USA. |
|
|
24. Praveena, P.E., Periasamy, S., Kumar, A.A. and Singh, N.
(2010) Cytokines profiles, apoptosis and pathology of
experimental Pasteurella multocida serotype A1 infection in
mice. Res. Vet. Sci., 89: 332-339.
http://dx.doi.org/10.1016/j.rvsc.2010.04.012
PMid:20447665 |
|
|
|
25. Samuelson, D.A. (2007) Textbook of Veterinary Histology.
Saunders Elsevier, Missouri. |
|
|
26. Jesse, F.F.A., Affandi, S.A., Osman, A.Y., Adamu, L.,
Zamri-saad, M., Wahid, A., Rahman, A., Sabri, J. and Saharee,
A.A. (2013) Clinico-pathological features in mice following
oral exposure to Pasteurella multocida B: 2. IOSR J. Agric.
Vet. Sci., 3(4): 35-39.
http://dx.doi.org/10.9790/2380-0343539 |
|
|
|
27. Jesse, F.F.A., Adamu, L., Osman, A.Y., Zakaria, Z.,
Abdullah, R., Zamri-saad, M. and Saharee, A.A. (2013)
Clinico-pathological responses of calves associated with
infection of Pasteurella multocida type B: 2 and the bacterial
lipopolysaccharide and outer membrane protein immunogens. Int.
J. Anim. Vet. Adv., 5(5): 190-198. |
|
|
28. Annas, S., Zamri-Saad, M., Jesse, F.F.A. and Zunita, Z.
(2014) New sites of localisation of Pasteurella multocida B: 2
in buffalo surviving experimental Haemorrhagic septicaemia.
BMC Vet. Res., 10(88): 1-7.
http://dx.doi.org/10.1186/1746-6148-10-88 |
|
|
|
29. Annas, S., Zamri-Saad, M., Abubakar, M.S., Jesse, F.F.A.
and Zunita, Z. (2014) Distribution of Pasteurella multocida B:
2 in the respiratory, gastrointestinal and urinary tracts of
buffaloes following experimental subcutaneous inoculation. J.
Vet. Sci. Technol., 5(3): 1-6. |
|
|
30. Rafidah, O. and Zamri-saad, M. (2013) Effect of
dexamethasone on protective efficacy of live gdh A derivative
Pasteurella multocida B: 2 vaccine. Asian J. Anim. Vet. Adv.,
8(3): 548-554.
http://dx.doi.org/10.3923/ajava.2013.548.554 |
|
