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R esearch
(Published online:
06-06-2015)
2.
Isolation and molecular characterization of Salmonella spp.
from chevon and chicken meat collected from different districts of
Chhattisgarh, India -
V. K. Naik, S. Shakya,
A. Patyal, N. E. Gade and Bhoomika
Veterinary World, 8(6): 702-706
doi:
10.14202/vetworld.2015.702-706
V. K.
Naik: Department
of Veterinary Public Health and Epidemiology, College of
Veterinary Science and Animal Husbandry, Chhattisgarh Kamdhenu
Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India; vetvivek8583@gmail.com
S.
Shakya: Department
of Veterinary Public Health and Epidemiology, College of
Veterinary Science and Animal Husbandry, Chhattisgarh Kamdhenu
Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India; shakyadurg@
gmail.com
A.
Patyal: Department
of Veterinary Public Health and Epidemiology, College of
Veterinary Science and Animal Husbandry, Chhattisgarh Kamdhenu
Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India; anilvet01@gmail.com
N. E.
Gade: Department
of Veterinary Physiology and Biochemistry, College of Veterinary
Science and Animal Husbandry, Chhattisgarh Kamdhenu Vishwa
Vidyalaya, Anjora, Durg, Chhattisgarh, India; nitingadeivri@gmail.com
Bhoomika: Department of Veterinary Public Health and
Epidemiology, College of Veterinary Science and Animal Husbandry,
Chhattisgarh Kamdhenu Vishwa Vidyalaya, Anjora, Durg,
Chhattisgarh, India;
sirsant.mannat.09@gmail.com
Received: 22-01-2015, Revised: 24-04-2015, Accepted: 30-04-2015,
Published online: 06-06-2015
Corresponding author:
A. Patyal, e-mail:
anilvet01@gmail.com
Citation:
Naik VK, Shakya S,
Patyal A, Gade NE, Bhoomika (2015) Isolation and molecular
characterization of Salmonella spp. from chevon and chicken
meat collected from different districts of Chhattisgarh, India,
Veterinary World 8(6): 702-706.
Abstract
Aim:
The aim was to assess the prevalence of Salmonella in
raw chevon and chicken meat sold in the retail meat shops situated
in and around Durg, Rajnandgaon, Dhamtari, Raipur, and Bilaspur
districts of Chhattisgarh. Studies were also conducted to find out
the antibiotic resistance in Salmonella isolates.
Materials and Methods: A total of 400 samples comprising of
200 chevon meat and 200 chicken meat samples were processed for
isolation of Salmonella and all isolates were further
confirmed on the basis of cultural and biochemical characters and
by targeting invA gene of Salmonella. All
Salmonella isolates were also examined for their antimicrobial
drug susceptibility/resistance pattern against commonly used
antibiotics.
Results: Out of 400 samples, the prevalence of Salmonella
in chevon and chicken meat was found 9% and 7% respectively,
with an overall prevalence of 8%. Polymerase chain reaction
targeting invA gene of Salmonella showed positive
result with 31 isolates. All 32 Salmonella isolates were
found to be highly sensitive to ciprofloxacin while 96.87%, 96.87%
and 93.75% were sensitive to gentamicin, imipenem, and ceftazidime,
respectively. 93.75% and 59.37% isolates were resistant to
erythromycin and oxytetracycline, respectively. Out of 32, 14
isolates had multiple antibiotic resistance index equal to or more
than 0.2.
Conclusion: Salmonella in chevon and chicken meat
samples is prevailing in the areas of sampling due to poor
hygienic conditions and also demonstrated the varied spectrum of
antimicrobial resistance, including several multiple drug
resistance phenotypes. Therefore, the present study emphasizes the
need for continued surveillance of zoonotic foodborne
pathogens including antimicrobial-resistant variants throughout
the food production chain.
Keywords: chevon, Chhattisgarh, chicken,
isolation, molecular characterization, Salmonella.
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