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              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
 
 
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distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
              
              
(Published online: 
              
              30-06-2015) 
              
              
              20.  
              
              A comparative therapeutic management of 
              anoestrus in buffaloes using insulin and GnRH - R. D. 
              Purkayastha, S. N. Shukla, O. P. Shrivastava and P. R. Kumar 
              
              Veterinary World, 8(6): 804-807   
              
   
                
                
doi: 
              10.14202/vetworld.2015.804-807   R. D. 
              Purkayastha: 
              
              Department of Veterinary Gynaecology & Obstetrics, College of 
              Veterinary Science & Animal Husbandry, Tripura, India;
              
              
              purkayasthard@gmail.com S. N. 
              Shukla: 
              Department of Veterinary Gynaecology and Obstetrics, College of 
              Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary 
              Science University, Jabalpur, Madhya Pradesh, India;
              
              
              snshukla2@gmail.com O. P. 
              Shrivastava: 
              Department of Veterinary Gynaecology and Obstetrics, College of 
              Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary 
              Science University, Jabalpur, Madhya Pradesh, India;
              
              
              vetopshri@gmail.com P. R. 
              Kumar: Department of Veterinary Gynaecology & Obstetrics, 
              Faculty of Veterinary & Animal Sciences, Banaras Hindu University, 
              Varanasi, Uttar Pradesh, India;
              
              dr.pranjan007@gmail.com   Received: 
              16-01-2015, Revised: 25-05-2015, Accepted: 01-06-2015, Published 
              online: 30-06-2015   
              
              
              Corresponding author:P. R. Kumar, e-mail: dr.pranjan007@gmail.com 
 
              Citation:Purkayastha RD, Shukla 
              SN, Shrivastava OP, Kumar PR (2015) A comparative therapeutic 
              management of anoestrus in buffaloes using insulin and GnRH, 
              Veterinary World 8(6): 804-807. 
 
              Abstract 
 Aim:
              Anoestrus is one of the most common functional disorders of 
              the reproductive cycle in buffaloes. In spite of technical 
              advancement, there is no single cure for the management of 
              anoestrus. Therefore, the aim of this study was to find out the 
              efficacy of gonadotropic releasing hormone (GnRH) and metabolic 
              hormone for the management of true anoestrus in buffaloes. 
              Materials and Methods: The experimental animals were selected 
              on the basis of history, gyneco-clinical examinations and 
              progesterone estimation. Deworming was done with Fenbendazole and 
              thereafter mineral mixture was given @ 50 g per animal per day for 
              10 days in all the selected buffaloes before the start of 
              treatment. The selected buffaloes were randomly divided into four 
              groups (n=25). In Group I, buffaloes were administered 20 μg of 
              buserelin intramuscularly. Buffaloes of Group II were administered 
              long-acting insulin @ 0.25 IU/Kg body weight subcutaneously for 5 
              consecutive days. In Group III, buffaloes were treated with a 
              combination of insulin and buserelin in the above-mentioned doses 
              whereas buffaloes of Group IV were kept as untreated control.
               
              Results: The higher oestrus induction (64% 
              vs. 28%) was found in Group III and differed significantly 
              (p<0.05) as compared to control group. The conception rate (69.23% 
              vs. 66.66%) was also found higher in Group III but did not differ 
              significantly among the treated groups. The mean time taken for 
              the onset of oestrus was recorded significantly shorter in insulin 
              (8.80±0.69) and GnRH (7.60±0.92 days) alone and as compared to 
              other (Group III, 14.43±0.83 and Group IV, 20.57±1.69 days) 
              groups.  
              Conclusion: The results of this study 
              indicated better fertility response using Insulin plus Buserelin 
              in true anoestrus buffaloes under field conditions. 
              Keywords: anoestrus, buffalo, buserelin, 
              gonadotropic releasing hormone, insulin. 
 
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