Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License

( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online: 30-06-2015)

20.  A comparative therapeutic management of anoestrus in buffaloes using insulin and GnRH - R. D. Purkayastha, S. N. Shukla, O. P. Shrivastava and P. R. Kumar

Veterinary World, 8(6): 804-807



   doi: 10.14202/vetworld.2015.804-807


R. D. Purkayastha: Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Tripura, India;

S. N. Shukla: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India;

O. P. Shrivastava: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India;

P. R. Kumar: Department of Veterinary Gynaecology & Obstetrics, Faculty of Veterinary & Animal Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India;


Received: 16-01-2015, Revised: 25-05-2015, Accepted: 01-06-2015, Published online: 30-06-2015


Corresponding author: P. R. Kumar, e-mail:

Citation: Purkayastha RD, Shukla SN, Shrivastava OP, Kumar PR (2015) A comparative therapeutic management of anoestrus in buffaloes using insulin and GnRH, Veterinary World 8(6): 804-807.

Aim: Anoestrus is one of the most common functional disorders of the reproductive cycle in buffaloes. In spite of technical advancement, there is no single cure for the management of anoestrus. Therefore, the aim of this study was to find out the efficacy of gonadotropic releasing hormone (GnRH) and metabolic hormone for the management of true anoestrus in buffaloes.

Materials and Methods: The experimental animals were selected on the basis of history, gyneco-clinical examinations and progesterone estimation. Deworming was done with Fenbendazole and thereafter mineral mixture was given @ 50 g per animal per day for 10 days in all the selected buffaloes before the start of treatment. The selected buffaloes were randomly divided into four groups (n=25). In Group I, buffaloes were administered 20 μg of buserelin intramuscularly. Buffaloes of Group II were administered long-acting insulin @ 0.25 IU/Kg body weight subcutaneously for 5 consecutive days. In Group III, buffaloes were treated with a combination of insulin and buserelin in the above-mentioned doses whereas buffaloes of Group IV were kept as untreated control.

Results: The higher oestrus induction (64% vs. 28%) was found in Group III and differed significantly (p<0.05) as compared to control group. The conception rate (69.23% vs. 66.66%) was also found higher in Group III but did not differ significantly among the treated groups. The mean time taken for the onset of oestrus was recorded significantly shorter in insulin (8.800.69) and GnRH (7.600.92 days) alone and as compared to other (Group III, 14.430.83 and Group IV, 20.571.69 days) groups.

Conclusion: The results of this study indicated better fertility response using Insulin plus Buserelin in true anoestrus buffaloes under field conditions.

Keywords: anoestrus, buffalo, buserelin, gonadotropic releasing hormone, insulin.

1. Kodagali, S.B. (1968) A note on reproductive disorders of farm animals in Saurshtra. Indian J. Vet. Sci. Anim. Husb., 38: 286-287.
2. Luktuke, S.N., Bhattacharyya, A.R., Singh, S.K. and Khan, B.V. (1973) Studies on aberration in functional activity of the ovaries in buffaloes. Indian Vet. J., 50: 876-881.
3. Kumar, P.R., Shukla, S.N., Shrivastava, O.P. and Purkayastha, R.D. (2013) Incidence of postpartum anoestrus among buffaloes in and around Jabalpur. Vet. World, 6(9): 716-719.
4. Thakor, D. and Patel, D. (2013) Incidence of infertility problems in cattle and buffaloes. Dairy Cattle. Available from htm. Accessed on 15-05-2015.
5. Kumar, P.R., Shukla, S.N. and Purkayastha, R.D. (2013) Economical analysis of the estimated cost of management of anoestrus buffaloes under field conditions using different hormonal and non–hormonal strategies. J. Anim. Health Prod., 1(4): 39-41.
6. Ghuman, S.P.S., Singh, J., Honparkhe, M., Dadarwal, D., Dhliwal, G.S. and Singh, S.T, (2010) Fate of dominant follicle in summer anoestrus buffaloes. Indian J. Anim. Reprod., 31(2): 7-10.
7. Das, G.K., Kumawat, B.L. and Khan, F.A. (2013) Ovarian follicular dynamics during estrous cycle and its aberrations during certain reproductive disorders in buffalo. Theriogenology, 3(1):37-46.
8. Peter, A.T., Vos, P.L.A. and Ambrose, D.J. (2009) Postpartum anoestrus in dairy cattle. Theriogenology, 71: 1333-1342.
9. Ramoun, A.A., Serur, B.H., Fattouh, El.S.M., Darweish, S.A., Abou, E1.G.H. (2012) Enhancing follicular growth as a prerequisite for GnRH treatment of true anoestrum in buffalo. Anim. Reprod. Sci., 132(1-2): 29-35.
10. Noakes, D.E., Parkinson, T.J. and England, Gary C.W. (2009) Veterinary Reproduction and Obstetrics. 9th ed. W.B. Saunders Company, London. p950.
11. Shukla, S.N., Agarwal, S.K., Shanker, U., Varshney, V.P. and Majumdar, A.C. (2005) Ovarian function and restoration of fertility using insulin in acyclic dairy cattle. Indian J. Anim. Sci., 75: 1135-1139.
12. Kumar, P.R., Shukla, S.N., Shrivastava, O.P., Mishra, A. and Purkayastha, R.D. (2013) Therapeutic management of true anoestrus in dairy buffaloes (Bubalus bubalis) using PMSG with metabolic hormone. Vet. Pract., 14(2): 323-324.
13. SPSS. (2010) Statistical Package for Social Sciences. Version 16, SPSS Inc., Illinois, USA.
14. Gupta, V., Thakur, M.S., Agrawal, R.G., Quadri M.A., and Shukla, S.N. (2010) Effect of pretreatment with Insulin on ovarian and fertility response in true anoestrus buffaloes to Gonadotrophin-Releasing hormone. Buffalo Bull., 29(3): 172-179.
15. Kezele, P.R., Nilson, E.E. and Skinner, M.K. (2002) Insulin but not insulin like growth factors-1 promotes the primordial to primary follicle transition. Mol. Cell Endocrinol., 198(1-2): 37-43.
16. Silva, J.R.V., Figueiredo, J.R. and Van den Hurk, R. (2009) Involvement of growth hormone and insulin-like growth factor system in ovarian folliculogenesis. Theriogenology, 71: 1193-1408.
17. Adsule, S.M., Baig, M.S., Gade, P.R. and Khandelwal, P.N. (2006) GnRH analogues and their role in gynaecology. Antiseptic, 103(5): 290-292.
18. Sirmour, S., Nema, S.P., Singh, B.K. and Shukla, S.P. (2006) Induction of oestrus in delayed pubertal crossbred heifer. Indian J. Anim. Reprod., 27(1): 55-58.
19. Puranik, P., Sadasiva Rao, K. and Solmon Raju, K.G. (2010) Effect of GnRH, PMSG and Placentrax on reproductive performance of post partum true anoestrus in Murrah buffaloes. Indian J. Anim. Res., 44(3): 201-204.
20. Kumar, P.R., Singh, S.K., Kharche, S.D., Govindaraju, C.S., Behera, B.K., Shukla, S.N., Kumar, H. and Agarwal, S.K. (2014) Anoestrus in cattle and Buffalo: Indian perspective. Adv. Anim. Vet. Sci., 2(3): 124-138.
21. McDougall, S. (2010) Effects of treatment of anestrous dairy cows with gonadotropin-releasing hormone, prostaglandin, and progesterone. J. Dairy Sci., 93(5): 1944-1959.
22. Singh, S.K., Ram, H. and Naresh, R. (2007) Induction of oestrus by insulin treatment in anoestrus cattle. XXIII Annual Convention of ISSAR and National Symposium. OP-36:339.
23. Palma, G.A., Muller, M. and Brem, G. (1997) Effect of insulin-like growth factor I (IGF-I) at high concentrations on blastocyst development of bovine embryos produced in vitro. J. Reprod. Fertil., 110: 347-353.
24. Baithalu, R.K., Singh, S.K., Gupta, C., Raja, A.K., Saxena, A. and Agarwal, S.K. (2013) Insulin stimulates progesterone secretion to a greater extent than LH in early pregnant buffalo luteal cells cultured in vitro. Anim. Reprod. Sci., 142: 131-136.