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R esearch
(Published online:
30-06-2015)
21.
Effect of supplementation of butylated
hydroxytoluene on post-thaw sperm viability, motility and membrane
integrity of Hariana bulls - Akhil Patel, Atul Saxena,
Dilip Kumar Swain, Dushyant Yadav, Sanjay Singh Yadav, Abhishek
Kumar and Anuj Kumar
Veterinary World, 8(6): 808-812
doi:
10.14202/vetworld.2015.808-812
Akhil
Patel:
Department of Veterinary Obstetrics & Gynaecology, College of
Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal
Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go
Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India;
dr.akhil.patel87@gmail.com
Atul
Saxena:
Department of Veterinary Obstetrics & Gynaecology, College of
Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal
Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go
Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India;
dratulsaxena@rediffmail.com
Dilip
Kumar Swain:
Department of Veterinary Physiology, College of Veterinary Science
& Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu
Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan,
Mathura - 281 001, Uttar Pradesh, India;
dilip_swain@yahoo.com
Dushyant Yadav:
Department of Veterinary Obstetrics & Gynaecology, College of
Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal
Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go
Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India;
drdushyantyadav52@gmail.com
Sanjay
Singh Yadav:
Department of Veterinary Obstetrics & Gynaecology, College of
Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal
Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go
Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India;
sanjay.yadav.vet@gmail.com
Abhishek Kumar:
Department of Veterinary Obstetrics & Gynaecology, College of
Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal
Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go
Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India;
Abhishekkumar712@gmail.com
Anuj
Kumar: Department of Veterinary Obstetrics & Gynaecology,
College of Veterinary Science & Animal Husbandry, U.P. Pandit
Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go
Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India;
anujduvasu@rediffmail.com
Received: 09-02-2015, Revised: 25-05-2015, Accepted: 01-06-2015,
Published online: 30-06-2015
Corresponding author:
Akhil Patel, e-mail: dr.akhil.patel87@gmail.com
Citation:
Patel A, Saxena A,
Swain DK, Yadav D, Yadav SS, Kumar A, Kumar A (2015) Effect of
supplementation of butylated hydroxytoluene on post-thaw sperm
viability, motility and membrane integrity of Hariana bulls,
Veterinary World 8(6): 808-812.
Abstract
Aim:
This study was aimed to see the beneficial effect of butylated
hydroxytoluene (BHT) as a semen additive of Hariana bull semen.
Materials and Methods: The study was carried out in Hariana
bulls. Twenty-four ejaculates from two bulls were used for this
study. Each ejaculate was extended with standard glycerolated egg
yolk tris extender and supplemented with BHT at two concentrations
as 0.5 mM (T1) and 1.0 mM (T2). After dilution, equilibration and
24 h of cryopreservation, the samples were analyzed for
progressive motility, sperm viability and membrane integrity.
Results: Progressive motility, sperm viability and sperm
membrane integrity were significantly (p<0.05) increased in the
samples fortified with BHT as compared to the control during the
process of cryopreservation and thawing. The BHT concentration of
1 mM revealed better results as compared to 0.5 mM.
Conclusion: Addition of 1.0 mM BHT was found better in
cryopreservation of Hariana bull semen compared to 0.5 mM BHT and
control samples. The addition of BHT has improved the sperm
quality by acting as an antioxidant thereby reducing the lipid
peroxidation of the sperms.
Keywords: sperm, viability, butylated
hydroxytoluene, membrane integrity, antioxidant, Hariana bull.
References
1. Watson, P.F. (2000) The causes of reduce fertility with
cryopreserved semen. Anim. Reprod. Sci., 60: 481-492.
http://dx.doi.org/10.1016/S0378-4320(00)00099-3 |
|
|
2. Thomas, A.D., Meyers, S.A. and Ball, B.A. (2006)
Capacitation – Like changes in equine spermatozoa following
cryopreservation. Theriogenology, 65(8): 1531-1550.
http://dx.doi.org/10.1016/j.theriogenology.2005.08.022
PMid:16225914 |
|
|
3. Thomson, L.K., Fleming, S.D., Aitken, R.J., De luliis,
G.N., Zieschang, J.A. and Clark, A.M. (2009)
Cryopreservation-induced human sperm DNA damage is
predominantly mediated by oxidative stress rather than
apoptosis. Hum. Reprod., 24(9): 2061-2070.
http://dx.doi.org/10.1093/humrep/dep214
PMid:19525298 |
|
|
4. Tremellen, K. (2008) Oxidative stress and male infertility
– A clinical perspective. Hum. Reprod. Update, 14(3): 243-258.
http://dx.doi.org/10.1093/humupd/dmn004
PMid:18281241 |
|
|
5. Ball, B.A., Medina, V., Gravance, C.G. and Bumber, J.
(2001) Effect of antioxidants on preservation of motility,
viability and acrosomal integrity of equine spermatozoa during
storage at 5 ◦C. Theriogenology,56: 577-589.
http://dx.doi.org/10.1016/S0093-691X(01)00590-8 |
|
|
|
6. Andreea, A. and Stela, Z. (2010) Role of antioxidant
additives in the protection of the cryopreserved semen against
free radicals. Roman Biotechnol. Lett., 15: 3-8. |
|
|
7. Khalifa, T.A.A., Lymberopoulos, A.G. and ElSaidy, B.E.
(2008) Testing usability of butylated hydroxytoluene in
conservation of goat semen. Reprod. Domest. Anim., 43:
525-530.
http://dx.doi.org/10.1111/j.1439-0531.2007.00947.x
PMid:18298403 |
|
|
|
8. Andrabi, S.M.H., Ansari, M.S., Ullah, N. and Afzal, M.
(2008) Effect of nonenzymatic antioxidants in extender on
post-thaw quality of buffalo (Bubalusbubalis) bull
spermatozoa. Pak. Vet. J., 28: 159-162. |
|
|
9. Neagu, V.R., MacıŽas GarcıŽa Garcia , B. M., Sandoval,
C.S., Morillo RodrıŽguez Rodriguez, A. M., Ferrusola, C.O.,
GonzaŽlez FernaŽndez Fernandez, L. G., Tapia, J.A. and Pen˜a
Pena, F.J. (2010) Freezing dog semen in presence of the
antioxidant butylatedhydroxytoluene improves post thaw sperm
membrane integrity. Theriogenology, 73(5): 645-650.
http://dx.doi.org/10.1016/j.theriogenology.2009.10.021
PMid:20018359 |
|
|
10. Farshad, A., Khalili, B. and Jafaroghli, M. (2010) Effects
of butylated hydroxytoluene on freezability of ram
spermatozoa. Asian-Aust. J. Anim., 23(10): 1276-1281.
http://dx.doi.org/10.5713/ajas.2010.90590 |
|
|
11. Fujisawa, S., Kadomab, Y. and Yokoe, I. (2004)
Radical-scavenging activity of butylated hydroxytoluene(BHT)
and its metabolites. Chem. Phys. Lipids, 130(2): 189-195.
http://dx.doi.org/10.1016/j.chemphyslip.2004.03.005
PMid:15172835 |
|
|
|
12. Swain, D.K., Swarnkar, P. and Yadav, S. (2012a) Hypo
osmotic swelling test of caprine epididymal sperms. Indian J.
Anim. Sci., 82(11): 1351-1352. |
|
|
|
13. Swain, D.K., Swarnkar, P., Kumar, J. and Yadav, S. (2012b)
Evaluation of in vitro longevity of caprine cauda epididymal
sperms at different storage intervals of time. Indian J. Anim.
Sci., 82(11): 1347-1350. |
|
|
14. Said, T.M., Gaglani, A. and Agarwal, A. (2010) Implication
of apoptosis in sperm cryoinjury. Reprod. Biomed. Online., 21:
456-462.
http://dx.doi.org/10.1016/j.rbmo.2010.05.011
PMid:20800544 |
|
|
|
15. Suttiyotin, P., Nakthong, C., Kimsakulvech, S. and
Jarearnkul, D. (2011) Effect of butylated hydroxytoluene on
survival of frozen-thawed fighting bull spermatozoa. J. Agric.
Sci. Technol., 1: 1177-1180. |
|
|
16. Ö˘gretmena, F. and Inanan, B.E. (2014) Effect of butylated
hydroxytoluene (BHT) on the cryopreservation of common carp
(Cyprinus carpio) spermatozoa. Anim. Reprod. Sci., 151:
269-274.
http://dx.doi.org/10.1016/j.anireprosci.2014.10.013
PMid:25459081 |
|
|
17. Baumber, J., Ball, B.A., Linfor, J.J. and Meyers, S.A.
(2003) Reactive oxygen species and cryopreservation promote
DNA fragmentation in equine spermatozoa. J. Androl., 24:
621-628.
PMid:12826702 |
|
|
18. Nordberg, J. and Amer, E.S. (2001) Reactive oxygen
species, antioxidants, and the mammalian thior edoxin system.
Free Radic. Bio. Med., 31: 1287-1312.
http://dx.doi.org/10.1016/S0891-5849(01)00724-9 |
|
|
19. Roca, J., Gilt, M.A., Hernandez, M., Parrilia, I.,
Vazquez, J.M. and Martinez, E.M. (2004) Survival and fertility
of boar spermatozoa after freez-thawing in extender
supplemented with butylated hydroxytoluene. J. Androl., 25:
397-405.
PMid:15064318 |
|
|
20. Shoae, A. and Zamiri, M.J. (2008) Effect of butylated
hydroxytoluene on bull spermatozoa frozen in egg yolk-citrate
extender. Anim. Reprod. Sci., 104: 414-418.
http://dx.doi.org/10.1016/j.anireprosci.2007.07.009
PMid:17826014 |
|
|
21. Naijian, H.R., Kohram, H., Shahneh, A.Z., Sharafi, M. and
Bucak, M.N. (2013) Effects of different concentrations of BHT
on microscopic and oxidative parameters of Mahabadi goat semen
following the freeze–thaw process. Cryobiology, 66: 151-155.
http://dx.doi.org/10.1016/j.cryobiol.2012.12.010
PMid:23313785 |
|
|
22. Ijaz, A., Hussain, A., Aleem, M., Yousaf, M.S. and Rehman,
H. (2009) Butylated hydroxytoluene inclusion in semen extender
improves the post-thawed semen quality of Nili-Ravi buffalo
(Bubalus bubalis). Theriogenology, 71: 1326-1329.
http://dx.doi.org/10.1016/j.theriogenology.2008.12.023
PMid:19246080 |
|
|
23. Stradaioli, G., Noro, T., Sylla, L. and Monaci, M. (2007)
Decrease in glutathione (GSH) content in bovine sperm after
cryopreservation: Comparison between two extenders. Anim.
Reprod. Sci., 67: 1249-2155.
http://dx.doi.org/10.1016/j.theriogenology.2007.01.009 |
|
