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Research (Published online: 30-06-2015)

22.  Hematocrit alterations and its effects in naturally infected indigenous cattle breeds due to Trypanosoma spp. on the Adamawa Plateau - Cameroon  - A. Mamoudou, V. K. Payne and S. L. Sevidzem

Veterinary World, 8(6): 813-818



   doi: 10.14202/vetworld.2015.813-818


A. Mamoudou: Department of Parasitology and Parasitological Diseases, School of Veterinary Medicine and Sciences, University of Ngaoundere, Cameroon;

V. K. Payne: Department of Animal Biology, Faculty of Science, University of Dschang BP 67 Dschang, Cameroon;

S. L. Sevidzem: Department of Animal Biology, Faculty of Science, University of Dschang BP 67 Dschang, Cameroon;


Received: 02-03-2015, Revised: 22-05-2015, Accepted: 02-06-2015, Published online: 30-06-2015


Corresponding author: A. Mamoudou, e-mail:

Citation: Mamoudou A, Payne VK, Sevidzem SL (2015) Haematocrit alterations and its effects in naturally infected indigenous cattle breeds due to Trypanosoma spp. on the Adamawa Plateau - Cameroon, Veterinary World 8(6): 813-818.

Aim: An experimental study was carried out on 148 naturally infected indigenous cattle breeds with either single or mix infections of various species of trypanosomes. The objectives of this study were to determine the species of trypanosomes, observe their hematopathological consequences on host-related risk factors and to determine the packed cell volume (PCV) of the infected group.

Materials and Methods: The buffy coat method (BCM) which is a variant of the hematocrit centrifugation method was used for the parasitological and hematological analysis. The May Grünwald-Giemsa method was also used for the identification of different trypanosome species.

Results: The infection rate in accordance with the various trypanosomes was as follows: Trypanosoma congolense + Trypanosoma brucei (1.35%), Trypanosoma vivax + T. brucei (1.35%), T. congolense + T. vivax (8.11%), T. congolense + T. vivax + T. brucei (8.78%), T. brucei brucei (11.48%), T. vivax (20.94%), T. congolense (47.97%). The infection rate with respect to breeds showed the following results - Brahman (1.0%), Red Fulani (5.2%), White Fulani (6.5%) and Gudali (16.7%), with no statistical significant difference (p>0.05). The combined mean PCV of single as well as mix infections was not statistically significant (p>0.05). The mean PCV of males (25.64±5.08 standard deviation [SD]) which was lower than that of females (30.82±4.94 SD) was statistically significant (p<0.05). The body condition of infected animals with sex showed that a greater proportion of males with "Poor" and "Medium" conditions showed high prevalence than females with the same conditions, with a significant difference (p<0.05). However, females showed a "Good" condition than males even though it was not statistically significant (p>0.05). The PCV profile of the infected group showed that the highest proportion of infected animals had PCV of ≤31% than PCV >31%. The mean weight of the animals was (265.41±95.36 SD). A scatter-linear plot of infected buffy coat against mean PCV showed a negative parametric correlation.

Conclusion: Distinguished Trypanosoma spp. pathogenicity, emaciation and weight loss related anemia, poor body condition, sex and the response of different breeds to various trypanosomes were highly affected and are of vital importance in diagnosis and act as a contribution to future control and treatment plans in this area.

Keywords: hematocrit, buffy coat method, body condition, anemia, trypanosome, breed, cattle, Adamaoua Plateau - Cameroon.

1. Hoare, A.C. (1972) The salivaria. In: The Trypanosomes of Mammals. A Zoological Monograph. Blackwell Scientific Publications Oxford and Edinburgh, Oxford, UK. p401-609.
2. Itard, J. (1989) African Animal Trypanonosomoses. In: Mannual of Tropical Veterinary Parasitology, Translated by Shah-Fischer, M., Ralph Say, R. Vol. 1. CAB International, Wallingford. p177-264.
3. Mpouam, S.E., Achukwi, M.D., Feussom Kameni, S.M., Bengaly, Z. and Ouedraogo, G.A. (2011) Serological and parasitological prevalence of bovine trypanosomiasis in small holder farms of the vina division, Adamawa Region of cameroon. J. Parasitol. Vect. Biol., 3(4): 44-51.
4. Mamoudou, A., Zoli, A. and Tchoua, P. (2009) Parasitological prevalence of bovine trypanosomosis in the faro and deo division valley of the Adamaoua plateau, cameroon. Int. J. Biol. Chem. Sci., 3(5): 1192-1197.
5. Achukwi, M.D. and Musongong, G.A. (2009) Trypanosomiasis in the dayo/namchi (Bos taurus) and zebu white fulani (Bos indicus) cattle in faro division, North Cameroon. J. Appl. Biosci., 15: 807-814.
6. Anosa, V.O. and Kaneko, J.J. (1983) Pathogenesis of Trypanosoma brucei infection in deer mice (Peromyscus maniculatus). V. Macrophage ultrastructure and function. Vet. Path., 20(5): 617-631.
7. Anosa, V.O. (1983) Mammalian blood cells in health and in trypanosomiasis. Trop. Vet., 1(4): 177-199.
8. Okaiyeto, S.O., Ajanusi, O.J., Sackey, A.K. and Tekdek. L.B. (2010) Changes in some haematological values associated with mixed Trypanosoma congolense and Haemonchus contortus infection in yankassa sheep. J. Vet. Res., 3(2): 9-13.
9. Silbermayr, K., Li, F., Soudre, A., Muller, S. and Solker, J. (2013) A novel q PCR assay for the detection of African animal Trypanosomiasis in Trypanotolerant and Trypanosusceptible cattle breeds. Plos Negl. Trop. Dis., 7(8): 2345.
PMid:23967357 PMCid:PMC3744421
10. Tasew, S. and Duguma, R. (2012) Cattle anaemia and Trypanosomiasis in Western Oromia state, Ethiopia. Rev. Med. Vet., 163(12): 581-588.
11. Fentahun, T. and Tekeba, M. (2013) Prevalence of bovine Trypanosomiasis and distribution of vectors in Hawa Gelan District, Oromia Region, Ethiopia., J. Vet. Adv., 3(8): 238-244.
12. FAO. (1980) Trypanotolerant livestock in West and central Africa. Anim. Prod. Health Paper., 2: 235-243.
13. Herd, D.B. and Sprott, L.R. (1986) Body Condition, Nutrition and Reproduction of Beef Cows. Texas Agricultural Extension Service. B-1526.
14. Khan, H., Sir Zamin, M., Rind, M., Rind, R. and Riaz, M. (2003) Use of shaeffer`s formula for the prediction of body weight of slaughtering cattle. J. Anim. Vet. Adv., 2(3): 176-178.
15. Murray, M., Murray, P.K. and McIntyre, W.I.M. (1977) An improved parasitological technique for the diagnosis of African trypanosomiasis. Trans. Royal S. Trop. Med. Hyg., 71: 325-326.
16. Benjamin, M.M. (1986) Outline of Veterinary Clinical Pathology. 3rd ed. The Lowa State Univercity Press, Almes, Lowa, U.S.A. p05-37.
17. Soulsby, E.T.L. (1982), Helminthes, Arthropods, and Protozoa of Domesticated Animals. 7th ed. Bailliere and Tindal, London. p532-533.
18. Ezeokonkwo, R.C., Ezeh, I.O., Onunkwo, J.I, Obi, P.O., Onyenwe, I.W. and Agu, W.E. (2012) Comparative haematological study of single and mixed infections of mongrel dogs with Trypanosoma congolense and Trypanosoma brucei brucei. Vet. Parasitol., 173(1-2): 48-54.
19. Terzu, D. and Getachew, A. (2008) Seasonal dynamics of tsetse and trypanosomiasis in selected sites of Southern nation, nationalities and people's regional state, Ethiopia. Ethiop. Vet. J., 12: 77-96.
20. El-Metanawey, T.M., Nadia, M.E., Abdel, El-Aziz, Hassane, M.S. and Abdel El-Aziz, T.H. (2009) Comparative studies on diagnosis of trypanosoma evansi in experimentally infected goats. Glob. Vet., 3(4): 348-353.
21. Eyasu, A. and Ahmed, Y. (2013) Prevalence of bovine trypanosomiasis in Wolaita zone Kindokoish District of Ethiopia. Afr. J. Agric. Res., 8(49): 6383-6387.
22. Kelly, W.R. (1967) Veterinary Clinical Diagnosis. 1st ed. Bailliere, Tindal and Cassell, London.
23. Girma, K., Meseret, T., Tilahun, Z., Haimanot, D., Firew, L., Tadele, K. and Zelalem, A. (2014) Prevalence of bovine Trypanosomosis, its vector density and distribution in and around arbaminch, gamogofa zone, Ethiopia. Acta Para Glob., 5(3): 169-176.
24. Mersha, C., Chemirew, A. and Basaznew, B. (2012) Hematopathology and haematological parametric alterations in indigenous cattle due to trypanosomiasis. Glob. Vet., 9(5): 546-551.
25. Mbahin, N., Zoli, A., Mamoudou, A., Tanenbe, C., Abah, S., Ghogomu, R.T., Nouala, S.F. and Njeumi, F. (2008) Parasitological prevalence of bovine Trypanosomiasis in faro and deo division cameroon, ten years after the tsetse eradication campaign. Bull. Anim. Health Prod. Afr., 56: 289-297.
26. Orenge, C.O., Munga, L., Kimwele, C.N., Kemp, S., Korol, A., Gibson, J.P., Hanotte, O. and Soller, M. (2012) Trypanotolerance in N'Dama x boran crosses under natural trypanosome challenge: Effect of test-year environment, gender, and breed composition. BMC Genet., 17: 13-87.
27. Yehunie, B., Wudu, T., Nuria, Y. and Sefinew. A. (2012) Prevalence of bovine trypanosomiasis in Wemberma district of West Gojjam zone. North West Ethiopia. Ethiop. Vet. J., 16(2): 41-48.