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R esearch
(Published online:
17-06-2015)
9.
Evaluation of subacute bisphenol – A
toxicity on male reproductive system - S. S. Karnam, R.
C. Ghosh, S. Mondal and M. Mondal
Veterinary World, 8(6): 738-744
doi:
10.14202/vetworld.2015.738-744
S. S.
Karnam: Department
of Veterinary Pathology, College of Veterinary Science and Animal
Husbandry, Chhattisgarh Kamdhenu
Vishwavidyalaya, Durg, Chhattisgarh, India; dr.shiv.vet82@gmail.com
R. C.
Ghosh: Department
of Veterinary Pathology, College of Veterinary Science and Animal
Husbandry, Chhattisgarh Kamdhenu
Vishwavidyalaya, Durg, Chhattisgarh, India; rcghosh@rediffmail.com
S.
Mondal: Department
of Veterinary Pathology, West Bengal University of Animal and
Fishery Sciences, Kolkata, West Bengal, India; vetsamiran@gmail.com
M.
Mondal: Department of Veterinary Pathology, College of
Veterinary Science and Animal Husbandry, Nanaji Deshmukh
Veterinary Science University Rewa, Madhya Pradesh, India;
drmondalmm@rediffmail.com
Received: 19-01-2015, Revised: 05-05-2015, Accepted: 13-05-2015,
Published online: 17-06-2015
Corresponding author:
S. S. Karnam, e-mail:
dr.shiv.vet82@gmail.com
Citation:
Karnam SS, Ghosh RC,
Mondal S, Mondal M (2015) Evaluation of subacute bisphenol - A
toxicity on male reproductive system, Veterinary World 8(6):
738-744.
Abstract
Aim:
The aim was to evaluate the effect of multiple oral
administration of bisphenol A (BPA) for 28 days on seminal
characteristic on mammal using Wistar rat as a model.
Materials and Methods: Rats were randomly divided into five
different groups having 6 male rats in each group. The doses
chosen were 50, 200, and 600 mg/kg body weight for Groups III, IV
and V, respectively, based on preliminary dose range finding study
and Group II served as vehicle control and Group I was negative
control.
Results: Reproductive study in the BPA-treated rats on day 28
revealed that there was significant (p ≤0.05)
reduction in the epididymal sperm count of rats of Group IV and
significant (p≤0.01)
decrease in Group V. Sperm motility percentage, dead count
percentage, head and tail abnormality percentage were found to be
significantly (p≤0.01)
increased in rats of BPA-treated groups as compared to rats of
control groups. Testes showed necrosis of germinal layer and
spermatogonial cells in the seminiferous tubules. Hematological
examination revealed significant (p≤0.01)
decrease in the mean values of total erythrocyte count (TEC),
total leukocyte count (TLC), hemoglobin, packed cell volume, and
there was also significant (p≤0.05)
lymphocytopenia in treated animals.
Conclusion: It can be concluded from this study that subacute
toxicity of BPA caused a reduction in the epididymal sperm count,
sperm motility, dead count, head and tail abnormality, as well as
hematological indices such as TLC, TEC etc. Hence, it appears that
BPA affects the germ cells leading to impairment in the
spermatogenesis, and thus having its property as reproductive
toxicant and it also suppresses bone marrow functioning, which
leads to normocytic hypochromic anemia in rats.
Keywords: bisphenol A, hematology, rat,
spermatozoa, testes.
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