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              Research 
              
(Published online: 
              12-03-2015) 
              
              10.
              
              Fertility response in postpartum anoestrus 
              buffaloes (Bubalus bubalis) using modified Ovsynch based 
              timed insemination protocols - 
              K. K. Gupta, S. N. 
              Shukla, P. Inwati and O. P. Shrivastava 
              
              Veterinary World, 8(3): 316-319   
              
   
                
                
doi: 
              10.14202/vetworld.2015.316-319   K. K. 
              Gupta: 
              Department of Veterinary Gynaecology and Obstetrics, College of 
              Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary 
              Science University, Jabalpur, Madhya Pradesh, India; kamalguptavet@gmail.com S. N. 
              Shukla: 
              Department of Veterinary Gynaecology and Obstetrics, College of 
              Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary 
              Science University, Jabalpur, Madhya Pradesh, India; snshukla2@gmail.com P. 
              Inwati: 
              Department of Veterinary Gynaecology and Obstetrics, College of 
              Veterinary Science & Animal Husbandry, Nanaji Deshmukh Veterinary 
              Science University, Jabalpur, Madhya Pradesh, India; inwativet@gmail.com O. P. 
              Shrivastava: Department of Veterinary Gynaecology and 
              Obstetrics, College of Veterinary Science & Animal Husbandry, 
              Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya 
              Pradesh, India;
              
              vetopshri@gmail.com   
              Received: 29-10-2014, Revised: 20-01-2015, Accepted: 28-01-2015, 
              Published online: 12-03-2015   
              
              
              Corresponding author:S. N. Shukla, e-mail: snshukla2@gmail.com 
 
              Citation:Gupta KK, Shukla SN, 
              Inwati P, Shrivastava OP (2015) Fertility response in postpartum 
              anoestrus buffaloes (Bubalus bubalis) using modified 
              Ovsynch based timed insemination protocols, Veterinary World
              8(3):316-319. 
 
              Abstract 
 
              Background and Aim: Suboestrus constitutes the largest factor 
              responsible for poor reproductive efficiency in buffaloes. 
              Therefore, oestrus synchronization (OS) and fixed-time artificial 
              insemination (AI) is considered an alternative approach to enhance 
              reproductive efficiency in buffaloes. Thus, the present study was 
              carried out to study the efficacy of modified Ovsynch protocol 
              with fixed time insemination in post-partum anoestrus buffaloes. 
              Materials and Methods: Total 50 post-partum anoestrus dairy 
              buffaloes were selected and randomly divided into 5 Groups, each 
              comprising ten animals (n=10). Animals of Group I received 
              buserelin acetate 10 μg(GnRH) at day 0 and 9, and prostaglandin 
              F2α (PGF2α) at day 7; in Group II similar to 
              Group I except double dose of Gn RH (20 μg) at day 0; in Group 
              III, similar to Group I plus additional administration of insulin 
              on day 0, 1 and 2; in Group IV, similar to Group II plus 
              additional administration of insulin on day 0,1 and 2; in Group V 
              similar to Group I except replacement of first Gn RH with insulin 
              on day 0,1 and 2. Animal of all groups were inseminated at fixed 
              time using frozen semen at 60 h and 72 h after PGF2α 
              administration and confirmed for pregnancy at day 60 
              post-insemination. 
              Results: The results revealed satisfactory 
              and comparable synchronization of oestrus (60-80%) and conception 
              rate (20-40%) among the various treatment groups in the present 
              study. The better synchronization was observed in modified 
              protocols. However, non-significantly higher conception rate was 
              recorded in modified Ovsynch protocol (40% each in Group II-V) as 
              compared to 20% in Group-I (p>0.05). 
              Conclusion: In this study comparatively higher OS and 
              conception following insulin modified Ovsynch based timed AI 
              protocols in post-partum anoestrus buffaloes was found 
              satisfactory and comparable. 
              Keywords: anoestrus buffaloes, fertility, 
              insulin, ovsynch synchronization, timed artificial insemination. 
 
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