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R esearch
(Published online:
16-03-2015)
13.
Biological and molecular characterization of
classical swine fever challenge virus from India -
Parveen Kumar, Vikramaditya Upmanyu and Pronab Dhar
Veterinary World, 8(3): 330-335
doi:
10.14202/vetworld.2015.330-335
Parveen
Kumar:
Division of Biological Standardization, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India;
vetsparveen@gmail.com
Vikramaditya Upmanyu:
Division of Biological Standardization, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India;
vupmanyu17@rediffmail.com
Pronab
Dhar: Division of Biological Standardization, Indian
Veterinary Research Institute, Izatnagar, Uttar Pradesh, India;
drpdhar@gmail.com
Received:
14-12-2014, Revised: 24-01-2015, Accepted: 29-01-2015, Published
online: 16-03-2015
Corresponding author:
Parveen Kumar, e-mail: vetsparveen@gmail.com
Citation:
Kumar P, Upmanyu V,
Dhar P (2015) Biological and molecular characterization of
classical swine fever challenge virus from India, Veterinary
World 8(3):330-335.
Abstract
Aim:
The aim of this study was biological and molecular
characterization of classical swine fever (CSF) challenge virus
from India.
Materials and Methods: CSF challenge virus maintained at
Division of Biological standardization was experimentally infected
to two seronegative piglets. The biological characterization was
done by clinical sign and symptoms along with postmortem findings.
For molecular characterization 5’-nontranslated region, E2 and
NS5B regions were amplified by reverse transcription polymerase
chain reaction and sequenced. The sequences were compared with
that of reference strains and the local field isolates to
establish a phylogenetic relation.
Results: The virus produced symptoms of acute disease in the
piglets with typical post-mortem lesions. Phylogenetic analysis of
the three regions showed that the current Indian CSF Challenge
virus is having maximum similarity with the BresciaX strain (USA)
and Madhya Pradesh isolate (India) and is belonging to subgroup
1.2 under Group 1.
Conclusion: Based on biological and
molecular characterization of CSF challenge virus from India is
described as a highly virulent virus belonging to subgroup 1.2
under Group 1 along with some field isolates from India and
Brescia strain.
Keywords: Classical Swine fever, Challenge
virus, molecular characterization, biological characterization,
phylogeny
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