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              Research 
              
(Published online: 
              18-03-2015) 
              
              15.
              
              Seroprevalence of bluetongue in ruminants of 
              Jharkhand - Pinky Tigga, Siddhartha Narayan Joardar, 
              Arkendu Halder, Chandan Lodh, Indranil Samanta, Devi Prasad Isore, 
              Kunal Batabyal and Samir Dey 
              
              Veterinary World, 8(3): 346-349   
              
   
                
                
doi: 
              10.14202/vetworld.2015.346-349   Pinky 
              Tigga: 
              Department of Veterinary Microbiology, West Bengal University of 
              Animal and Fishery Sciences, Belgachia, Kolkata - 700 037, West 
              Bengal, India;
              
              
              pinky.tigga@gmail.com 
              Siddhartha Narayan Joardar: 
              
              Department of Veterinary Microbiology, West Bengal University of 
              Animal and Fishery Sciences, Belgachia, Kolkata - 700 037, West 
              Bengal, India;
              
              
              joardar69@gmail.com Arkendu 
              Halder: 
              Department of Veterinary Microbiology, West Bengal University of 
              Animal and Fishery Sciences, Belgachia, Kolkata - 700 037, West 
              Bengal, India;
              
              
              arkenduhalder@gmail.com Chandan 
              Lodh: 
              Department of Veterinary Medicine, Ethics and Jurisprudence, 
              Faculty of Veterinary and Animal Sciences, Belgachia, Kolkata - 
              700 037, West Bengal, India;
              
              
              chand_lodh@rediffmail.com 
              Indranil Samanta: 
              Department of Veterinary Microbiology, West Bengal University of 
              Animal and Fishery Sciences, Belgachia, Kolkata - 700 037, West 
              Bengal, India;
              
              
              isamanta76@gmail.com Devi 
              Prasad Isore: 
              Department of Veterinary Microbiology, West Bengal University of 
              Animal and Fishery Sciences, Belgachia, Kolkata - 700 037, West 
              Bengal, India;
              
              
              deviprasadisore@gmail.com Kunal 
              Batabyal: 
              Department of Veterinary Microbiology, West Bengal University of 
              Animal and Fishery Sciences, Belgachia, Kolkata - 700 037, West 
              Bengal, India;
              
              
              drkb.micro@gmail.com Samir 
              Dey: Department of Veterinary Microbiology, West Bengal 
              University of Animal and Fishery Sciences, Belgachia, Kolkata - 
              700 037, West Bengal, India;
              
              samirdey74@rediffmail.com   Received: 
              14-11-2014, Revised: 03-02-2015, Accepted: 10-02-2015, Published 
              online: 18-03-2015   
              
              
              Corresponding author: 
              Siddhartha Narayan Joardar, e-mail: joardar69@gmail.com 
 
              Citation:Tigga P, Joardar SN, 
              Halder A, Lodh C, Samanta I, Isore DV, Batabyal K, Dey S (2015) 
              Seroprevalence of bluetongue in ruminants of Jharkhand, 
              Veterinary World 8(3):346-349. 
 
              Abstract 
 Aim:
              This study was carried out to assess the presence of 
              anti-bluetongue (BT) antibodies in sheep, goat and cattle of 
              different agro-climatic zones of Jharkhand. 
              Materials and Methods: Serum samples were collected from 
              apparently healthy as well as suspected sheep, goat and cattle 
              from different districts of Jharkhand covering different 
              agro-climatic zones. Serum samples were screened by indirect 
              enzyme linked immunosorbent assay (iELISA) for detecting anti-BT 
              antibodies. 
              Results: Out of a total of 480 animal serum samples 
              (sheep-190, goats-210 and cattle-80) screened, 83 (43.68%) of 
              sheep, 91 (43.33%) of goat and 46 (57.50%) of cattle sera were 
              found positive. The % positivity ranged between 41% and 51% in 
              different agro-climatic zones. The results showed slight higher 
              seroprevalence, although not significantly, in cattle than sheep 
              and goats in different agro-climatic zones of Jharkhand. 
              Conclusions: The above data indicate widespread prevalence of 
              BT virus antibodies in studied areas. The incidence of BT is not 
              detected officially, so far. The present seroprevalence status of 
              BT in Jharkhand indicates presence of BT infection in the state 
              for the first time. 
              Keywords: antibodies, bluetongue, indirect 
              enzyme linked immunosorbent assay, Jharkhand, seroprevalence, 
              virus. 
 
              References 
 
                
                  | 1. Afshar, A. (1994) Bluetongue: Laboratory diagnosis. Comp. 
                  Immunol. Microbiol. Infect. Dis., 17: 221-242. http://dx.doi.org/10.1016/0147-9571(94)90045-0
 |  
                  |  |  
                  | 2. Gard, G.P. and Melville, L.E. (1992) In: Walton, T.E. and 
                  Osburn, B.I., editors. Bluetongue, African Horse Sickness and 
                  Related Orbiviruses. C.R.C. Press, Boca Raton. USA. p85. |  
                  |  |  
                  | 3. Wilson, A.A., Chitra, S. and Palaniswami, K.S. (2000) 
                  Bluetongue in India-Present Scenario. Intas Polivet, 1: 
                  209-212. |  
                  |  |  
                  | 4. Maan, S., Maan, N.S., Nomikou, K., Veronesi, E., 
                  Bachanek-Bankowska, K., Belaganalli, M.N., Attoui, H. and 
                  Mertens, P.C. (2011) Complete genome characterization of a 
                  novel 26th bluetongue virus serotype from Kuwait. PLoS One, 
                  6(10), e26147. http://dx.doi.org/10.1371/journal.pone.0026147
 PMid:22031822 PMCid:PMC3198726
 |  
                  |  |  
                  | 5. Joardar, S.N., Lodh, C., Chakrabarti, A., Baksi, S. and 
                  Aich, R. (2009) Isolation of bluetongue virus serotypes 15 and 
                  21 in West Bengal, India. Vet. Rec., 165: 751-752. PMid:20023280
 |  
                  |  |  
                  | 6. Rajkhowa, S., Rajkhowa, C., Dutta, P.R., Michuui, P. and 
                  Das, R. (2008) Bluetongue infection rate in Mithun (Bos 
                  frontalis) in the north-eastern upland region of India. Rev. 
                  Sci. Technol., 27(3): 907-914. PMid:19284059
 |  
                  |  |  
                  | 7. Joardar, S.N., Ibotombi, S.N., Barkataki, B., Lodh, C., 
                  Chakraborti, A. and Pradhan, N.R. (2012) Seroprevalence of 
                  bluetongue in ruminants of Manipur. Indian J. Comp. Microbiol. 
                  Immunol. Infect. Dis., 33(1&2): 54-56. |  
                  |  |  
                  | 8. Mellor, P.S., Boorman, J. and Baylis, M. (2000) Culicoides 
                  biting midges: Their role as arbovirus vectors. Annu. Rev. 
                  Entomol., 45: 307-340. http://dx.doi.org/10.1146/annurev.ento.45.1.307
 PMid:10761580
 |  
                  |  |  
                  | 9. Standfast, H.A., Dyce, A.L. and Muller, M.J. (1985) Vectors 
                  of BT in Australia. In: Barber, T.L. and Jochim, M.M., 
                  editors. Bluetongue and Related Orbiviruses. Alan R. Liss, 
                  Inc., New York, USA. p177-186. PMid:2989850
 |  
                  |  |  
                  | 10. Gibbs, E.P. and Greiner, E.C. (1994) The epidemiology of 
                  bluetongue. Comp. Immunol. Microbiol. Infect. Dis., 17: 
                  207-220. http://dx.doi.org/10.1016/0147-9571(94)90044-2
 |  
                  |  |  
                  | 11. Sapre, S.N. (1964) An outbreak of bluetongue in goats and 
                  sheep. Indian Vet. Rev., 15: 78-80. |  
                  |  |  
                  | 12. De, A., Batabyal, S., Biswas, S.K., Chand, K., Singh, R.K. 
                  and Mondal, B. (2008) Surveillance of bluetongue virus 
                  antibody in goats using a recombinant VP7-based indirect ELISA 
                  in the coastal saline area of West Bengal, India. Vet. Ital., 
                  45: 339-346. |  
                  |  |  
                  | 13. Prasad, G. and Srivastava, R.N. (1995) Epizootiology of 
                  bluetongue virus in particular reference to India. In: Prasad, 
                  G. and Srivastava, R.N., editors. Bluetongue: Indian 
                  Perspective. CCS Haryana Agricultural University, Hisar. p1-3. |  
                  |  |  
                  | 14. Sreenivasulu, D. and Subba Rao, M.V. (1999) 
                  Seroepidemiology of bluetongue disease in Andhra Pradesh. 
                  Indian J. Anim. Sci., 69: 292. |  
                  |  |  
                  | 15. MacLachlan, N.J., Nunamaker, R.A., Katz, J.B., Sawyer, 
                  M.M., Akita, G.Y., Osburn, B.I. and Tabachnick, W.J. (1994) 
                  Detection of bluetongue virus in the blood of inoculated 
                  calves: Comparison of virus isolation, PCR assay, and in vitro 
                  feeding of Culicoides variipennis. Arch. Virol., 136: 1-8. http://dx.doi.org/10.1007/BF01538812
 PMid:8002778
 |  
                  |  |  
                  | 16. Joardar, S.N., Barkataki, B., Halder, A., Lodh, C. and 
                  Sarma, D. (2013) Seroprevalence of bluetongue in north eastern 
                  Indian state-Assam. Vet. World, 6(4): 196-199. http://dx.doi.org/10.5455/vetworld.2013.196-199
 |  
                  |  |  
                  | 17. Dayakar, S., Sreenivasulu, D., Sudharani, K., Subba Rao, 
                  M.V. and Sreenivasulu, P. (2001) Serological survey for the 
                  prevalence of bluetongue virus antibodies in cattle by 
                  competitive enzyme linked immunosorbent assay. Indian J. Anim. 
                  Sci., 71(7): 614-616. |  
                  |  |  
                  | 18. Panda, M.K., Mondal, A., Joardar, S.N. (2011) 
                  Seroprevalence of bluetongue virus in sheep, goat and cattle 
                  in west Bengal, India. Anim. Sci. Reprod., 5(3): 105-110. |  
                  |  |  
                  | 19. Shlash Khalid, H., Laith, M.S., Abdul-Rasoul, M.M., Naji, 
                  M. and Hussain, H. (2012) Seroprevalence of bluetongue in 
                  Irawue. In: Proceeding of the Eleventh Veterinary Scientific 
                  Conference. p89-94. |  
                  |  |  
                  | 20. Naresh, A. and Prasad, G. (1995) Relative superiority of 
                  c-ELISA for detection of bluetongue virus antibodies. Indian 
                  J. Exp. Biol., 33(11): 880-882. PMid:8786164
 |  
                  |  |  
                  | 21. Chauhan, H.C., Chandel, B.S., Vasava, K.A., Patel, A.R., 
                  Singh, N.M. and Kher, H.N. (2004) Seroprevalence of bluetongue 
                  in Gujarat. Indian J. Comp. Microbiol. Immunol. Infect. Dis., 
                  25: 80-83. |  
                  |  |  
                  | 22. Singh, A., Agrawal, R., Singh, R., Singh, R.K. and Pande, 
                  N. (2009) Indirect-ELISA based on recombinant Vp7 specific 
                  protein for sero-epidemiological investigation of bluetongue 
                  in small ruminants of Jammu province. J. Immunol. 
                  Immunopathol., 11(2): 52-55. |  
                  |  |  
                  | 23. Noaman, V., Shirvani, E., Hosseini, S.M., Shahmoradi, 
                  A.H., Heideri, M.R., Raiszade, H., Kamalzade, M. and 
                  Bahreyari, M. (2013) Serological surveillance of bluetongue 
                  virus in central Iran. Vet. Ital., 49(2): 141-144. PMid:23888413
 |  |