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Research (Published online: 21-03-2015)

18. Somatic cell count and alkaline phosphatase activity in milk for evaluation of mastitis in buffalo - M. P. Patil, A. S. Nagvekar, S. D. Ingole, S. V. Bharucha and V. T. Palve

Veterinary World, 8(3): 363-366

 

 

   doi: 10.14202/vetworld.2015.363-366

 

M. P. Patil: Department of Veterinary Physiology, College of Animal Husbandry, Sharadanagar, Baramati, Pune, Maharashtra, India; patilmegha21@gmail.com

A. S. Nagvekar: Department of Veterinary Physiology, Bombay Veterinary College, Maharashtra Animal and Fishery Sciences University, Parel, Mumbai, Maharashtra, India; Anagha.nagvekar@gmail.com

S. D. Ingole: Department of Veterinary Physiology, Bombay Veterinary College, Maharashtra Animal and Fishery Sciences University, Parel, Mumbai, Maharashtra, India; ingoleshailesh@gmail.com

S. V. Bharucha: Department of Veterinary Physiology, Bombay Veterinary College, Maharashtra Animal and Fishery Sciences University, Parel, Mumbai, Maharashtra, India; Simin1972@gmail.com

V. T. Palve: Department of Veterinary Physiology, Bombay Veterinary College, Maharashtra Animal and Fishery Sciences University, Parel, Mumbai, Maharashtra, India; drvijaypalve88@gmail.com

 

Received: 04-10-2014, Revised: 24-01-2014, Accepted: 02-02-2015, Published online: 21-03-2015

 

Corresponding author: M. P. Patil, e-mail: patilmegha21@gmail.com


Citation: Patil MP, Nagvekar AS, Ingole SD, Bharucha SV, Palve VT (2015) Somatic cell count and alkaline phosphatase activity in milk for evaluation of mastitis in buffalo, Veterinary World 8(3);363-366.



Background and Aim: Mastitis is a serious disease of dairy animals causing great economic losses due to a reduction in milk yield as well as lowering its nutritive value. The application of somatic cell count (SCC) and alkaline phosphatase activity in the milk for diagnosis of mastitis in buffalo is not well documented. Therefore, the present study was conducted to observe the SCC and alkaline phosphatase activity for evaluation of mastitis in buffalo.

Materials and Methods: Milk samples of forty apparently healthy lactating buffaloes were selected and categorized into five different groups viz. normal buffaloes, buffaloes with subclinical mastitis with CMT positive milk samples (+1 Grade), (+2 Grade), (+3 Grade), and buffaloes with clinical mastitis with 8 animals in each group. The milk samples were analyzed for SCC and alkaline phosphatase activity.

Results: The levels of SCC (105 cells/ml) and alkaline phosphatase (U/L) in different groups were viz. normal (3.210.179, 16.481.432), subclinical mastitis with CMT positive milk samples with +1 Grade (4.210.138, 28.111.013), with +2 Grade (6.340.183, 34.501.034), with +3 Grade (7.960.213, 37.730.737) and buffaloes with clinical mastitis (10.210.220, 42.370.907) respectively, indicating an increasing trend in the values and the difference observed among various group was statistically significant.

Conclusion: In conclusion, the results of the present study indicate that the concentration of milk SCC and alkaline phosphatase activity was higher in the milk of buffaloes with mastitis than in the milk of normal buffaloes.

Keywords: alkaline phosphatase, buffalo milk, mastitis, somatic cell count.



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