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R esearch
(Published online: 21-03-2015)
19.
Production and assay of cellulolytic enzyme
activity of Enterobacter cloacae WPL 214 isolated from
bovine rumen fluid waste of Surabaya abbatoir, Indonesia -
W. P.
Lokapirnasari, D. S. Nazar, T. Nurhajati, K. Supranianondo and A.
B. Yulianto
Veterinary World, 8(3): 367-371
doi:
10.14202/vetworld.2015.367-371
W. P.
Lokapirnasari:
Department of Animal Husbandry, Faculty of Veterinary Medicine,
Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya,
Indonesia;
wp_lokapirnasari@yahoo.com
D. S.
Nazar:
Department of Animal Husbandry, Faculty of Veterinary Medicine,
Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya,
Indonesia;
dady_sn_drh@yahoo.com
T.
Nurhajati:
Department of Animal Husbandry, Faculty of Veterinary Medicine,
Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya,
Indonesia;
tri_nurhajati@yahoo.com
K.
Supranianondo:
Department of Animal Husbandry, Faculty of Veterinary Medicine,
Airlangga University, Jl. Mulyorejo, Campus C Unair,
Surabaya,
Indonesia; koesnotosp@yahoo.com
A. B.
Yulianto: Faculty of Veterinary Medicine, Wijaya Kusuma
Surabaya University, Jl. Dukuh Kupang Barat XVI/1 Surabaya,
Indonesia;
bernyjuliantomiroen@gmail.com
Received:
12-11-2014, Revised: 27-01-2015, Accepted: 05-02-2015, Published
online: 21-03-2015
Corresponding author:
W. P. Lokapirnasari, e-mail: wp_lokapirnasari@yahoo.com
Citation:
Lokapirnasari WP,
Nazar DS, Nurhajati T, Supranianondo K, Yulianto AB (2015)
Production and assay of cellulolytic enzyme activity of
Enterobacter cloacae WPL 214 isolated from bovine rumen fluid
waste of Surabaya abbatoir, Indonesia, Veterinary World 8(3):
367-371.
Abstract
Aim:
This study aims to produce and assay cellulolytic enzyme
activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-Dglucanase,
and β-glucosidase, at optimum temperature and optimum pH)
of Enterobacter cloacae WPL 214 isolated from bovine rumen
fluid waste of Surabaya Abbatoir, Indonesia.
Materials and Methods: To produce enzyme from a single colony
of E. cloacae WPL 214, 98 × 10 10 CFU/ml
of isolates was put into 20 ml of liquid medium and incubated in a
shaker incubator for 16 h at 35°C in accordance with growth
time and optimum temperature of E. cloacae WPL 214. Further on,
culture was centrifuged at 6000 rpm at 4°C for 15 min. Pellet was
discarded while supernatant containing cellulose enzyme activity
was withdrawn to assay endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase,
and β-glucosidase.
Results: Cellulase enzyme of E. cloacae WPL 214
isolates had endoglucanase activity of 0.09 U/ml, exoglucanase of
0.13 U/ml, and cellobiase of 0.10 U/ml at optimum temperature 35°C
and optimum pH 5.
Conclusion: E. cloacae WPL 214
isolated from bovine rumen fluid waste produced cellulose enzyme
with activity as cellulolytic enzyme of endo-(1,4)-β-D-glucanase,
exo-(1,4)-β-D-glucanase and β-glucosidase.
Keywords: endo-(1,4)-β -D-glucanase, exo-(1,4)-β-D-glucanase,
β-glucosidase., Enterobacter cloacae WPL 214.
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