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Research (Published online: 21-03-2015)

19. Production and assay of cellulolytic enzyme activity of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya abbatoir, Indonesia - W. P. Lokapirnasari, D. S. Nazar, T. Nurhajati, K. Supranianondo and A. B. Yulianto

Veterinary World, 8(3): 367-371

 

 

   doi: 10.14202/vetworld.2015.367-371

 

W. P. Lokapirnasari: Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya, Indonesia; wp_lokapirnasari@yahoo.com

D. S. Nazar: Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya, Indonesia; dady_sn_drh@yahoo.com

T. Nurhajati: Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Campus C Unair, Surabaya, Indonesia; tri_nurhajati@yahoo.com

K. Supranianondo: Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Campus C Unair,

Surabaya, Indonesia; koesnotosp@yahoo.com

A. B. Yulianto: Faculty of Veterinary Medicine, Wijaya Kusuma Surabaya University, Jl. Dukuh Kupang Barat XVI/1 Surabaya, Indonesia; bernyjuliantomiroen@gmail.com

 

Received: 12-11-2014, Revised: 27-01-2015, Accepted: 05-02-2015, Published online: 21-03-2015

 

Corresponding author: W. P. Lokapirnasari, e-mail: wp_lokapirnasari@yahoo.com


Citation: Lokapirnasari WP, Nazar DS, Nurhajati T, Supranianondo K, Yulianto AB (2015) Production and assay of cellulolytic enzyme activity of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya abbatoir, Indonesia, Veterinary World 8(3): 367-371.



Aim: This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-Dglucanase, and β-glucosidase, at optimum temperature and optimum pH) of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia.

Materials and Methods: To produce enzyme from a single colony of E. cloacae WPL 214, 98 1010 CFU/ml of isolates was put into 20 ml of liquid medium and incubated in a shaker incubator for 16 h at 35C in accordance with growth time and optimum temperature of E. cloacae WPL 214. Further on, culture was centrifuged at 6000 rpm at 4C for 15 min. Pellet was discarded while supernatant containing cellulose enzyme activity was withdrawn to assay endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase.

Results: Cellulase enzyme of E. cloacae WPL 214 isolates had endoglucanase activity of 0.09 U/ml, exoglucanase of 0.13 U/ml, and cellobiase of 0.10 U/ml at optimum temperature 35C and optimum pH 5.

Conclusion: E. cloacae WPL 214 isolated from bovine rumen fluid waste produced cellulose enzyme with activity as cellulolytic enzyme of endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase and β-glucosidase.

Keywords: endo-(1,4)-β -D-glucanase, exo-(1,4)-β-D-glucanase, β-glucosidase., Enterobacter cloacae WPL 214.



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