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R esearch
(Published online: 23-03-2015)
22.
Detection of Salmonella spp. from
chevon, mutton and its environment in retail meat shops in Anand
city (Gujarat), India -
P. P. Makwana, J. B.
Nayak, M. N. Brahmbhatt and J. H. Chaudhary
Veterinary World, 8(3): 388-392
doi:
10.14202/vetworld.2015.388-392
P. P.
Makwana:
Department of Veterinary Public Health and Epidemiology, Anand
Veterinary College, Anand Agricultural University, Anand, Gujarat,
India;
Paresh.makwana1989@gmail.com
J. B.
Nayak:
Department of Veterinary Public Health and Epidemiology, Anand
Veterinary College, Anand Agricultural University, Anand, Gujarat,
India;
jbn_anand@yahoo.com
M. N.
Brahmbhatt:
Department of Veterinary Public Health and Epidemiology, Anand
Veterinary College, Anand Agricultural University, Anand, Gujarat,
India;
mnbrahmbhatt2003@yahoo.com
J. H.
Chaudhary: Department of Veterinary Public Health and
Epidemiology, Anand Veterinary College, Anand Agricultural
University, Anand, Gujarat, India;
jitvph007@gmail.com
Received: 18-11-2014, Revised: 01-02-2015, Accepted: 10-02-2015,
Published online: 23-03-2015
Corresponding author:
P.P. Makwana, e-mail: Paresh.makwana1989@gmail.com
Citation:
Makwana PP, Nayak JB,
Brahmbhatt MN, Chaudhary JH (2015) Detection of Salmonella
spp. from chevon, mutton and its environment in retail meat shops
in Anand city (Gujarat), India, Veterinary World 8(3):
388-392.
Abstract
Aim:
The aim of this study was (i) To attempt isolation and
identification of Salmonella species from samples. (ii)
Serotyping of Salmonella isolates. (iii) Detection of
virulence factor associated genes by polymerase chain reaction (PCR).
Materials and Methods: A total of 284 samples comprised of
chevon and mutton (112 samples each) as well as 60 samples (20
each of retail meat shops environment samples viz.
Butchers’ hands, knives and log swabs) were collected from the
retail meat shops in and around Anand City under aseptic
precautions. Rappaport-vassiliadis soy bean meal broth and
tetrathionate broth was used for the enrichment of all the samples
and inoculation was done on brilliant green agar and xylose lysine
deoxycholate agar. This was followed by the confirmation of
isolates using biochemical tests. For the serotyping, isolates
were sent to the National Salmonella and Escherichia Centre,
Central Research Institute, Kasauli, Himachal Pradesh. Detection
of virulence genes was performed by PCR technique using previously
reported primer.
Result: Of 284 meats and retail meat shops
environment samples, 13 (4.58%) samples were found positive for
Salmonella. It was interesting to know that incidence of
Salmonella was more in mutton (6.25%) than chevon (3.57%). In
case of meat shop environmental samples 1 (5.00%) sample observed
positive for Salmonella separately among the butchers’
hands and knives swabs (Each of 20 samples) examined. Out of 13,
eleven isolates detected as Salmonella Typhimurium,
whereas only two isolates were detected as Salmonella
Enteritidis. All Salmonella isolates possess invA
and stn genes, whereas nine isolates had a presence of
spvR gene while only five of the isolates revealed the
presence of spvC gene as shown by in vitro detection
of virulence genes by PCR.
Conclusion: Therefore, might be suggested that the good
hygiene practices and effective control measures should be taken
to encourage clean meat production with prolonged shelf-life.
Keywords: food safety, meat, prevalence,
Salmonella spp, serotype.
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