Veterinary World

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Research (Published online: 23-03-2015)

23. Development of on package indicator sensor for real-time monitoring of meat quality - Vivek Shukla, G. Kandeepan and M. R. Vishnuraj

Veterinary World, 8(3): 393-397



   doi: 10.14202/vetworld.2015.393-397


Vivek Shukla: Division of Livestock Products Technology, Indian Veterinary Research Institute, Bareilly, Izatnagar, Uttar Pradesh, India;

G. Kandeepan: Division of Livestock Products Technology, Indian Veterinary Research Institute, Bareilly, Izatnagar, Uttar Pradesh, India;

M. R. Vishnuraj: Division of Livestock Products Technology, Indian Veterinary Research Institute, Bareilly, Izatnagar, Uttar Pradesh, India;


Received: 17-11-2014, Revised: 04-02-2015, Accepted: 13-02-2015, Published online: 23-03-2015


Corresponding author: G. Kandeepan, e-mail:

Citation: Shukla V, Kandeepan G, Vishnuraj MR (2015) Development of on package indicator sensor for real-time monitoring of meat quality, Veterinary World, 8(3): 393-397.

Aim: The aim was to develop an indicator sensor for real-time monitoring of meat quality and to compare the response of indicator sensor with meat quality parameters at ambient temperature.

Materials and Methods: Indicator sensor was prepared using bromophenol blue (1% w/v) as indicator solution and filter paper as indicator carrier. Indicator sensor was fabricated by coating indicator solution onto carrier by centrifugation. To observe the response of indicator sensor buffalo meat was packed in polystyrene foam trays covered with PVC film and indicator sensor was attached to the inner side of packaging film. The pattern of color change in indicator sensor was monitored and compared with meat quality parameters viz. total volatile basic nitrogen, D-glucose, standard plate count and tyrosine value to correlate ability of indicator sensor for its suitability to predict the meat quality and storage life.

Results: The indicator sensor changed its color from yellow to blue starting from margins during the storage period of 24 h at ambient temperature and this correlated well with changes in meat quality parameters.

Conclusions: The indicator sensor can be used for real-time monitoring of meat quality as the color of indicator sensor changed from yellow to blue starting from margins when meat deteriorates with advancement of the storage period. Thus by observing the color of indicator sensor quality of meat and shelf life can be predicted.

Keywords: buffalo meat, indicator sensor, meat quality, total volatile basic nitrogen.

1. Smigic, N., Djekic, I., Tomasevic, I., Miocinovic, J. and Gvozdevonic, R. (2012) Implications of food safety measures on microbiological quality of raw and pasteurized milk. Food Control, 25: 728-731.
2. Duncan, T.V. (2011) Application of nanotechnology in food packaging and food safety: Barrier materials, antimicrobials and sensors. J. Colloid Interface Sci., 363: 1-24.
3. European Commission. (2009) EU Guidance to Commission Regulation on Active and Intelligent Materials and articles Intended to Come into Contact with Food. European Commission.
4. Vanderroost, M., Ragaert, P., Devlieghere, F. and Meulenaer, B.D. (2014) Intelligent food packaging: The next generation. Trends Food Sci. Technol., 39: 47-62.
5. Vivancos, J.L., Manez, R.M., Lorente, I. and Gracia, E. (2014) A chromogenic sensor array for boiled marinated turkey. Sens. Actuators B Chem.,190: 326-333.
6. Pereira, V.C. Jr, Arruda, I.N.Q. and Stefani, R. (2014) Active chitosan/PVAfilms with anthocyanins from Brassica oleraceae (Red Cabbage) as time temperature indicators for application in intelligent food packaging. Food Hydrocoll., 43: 180-188.
7. Wanihsuksombat, C, Hongtrakul, V. and Suppakul, P. (2010) Development and characterization of a prototype of a lactic acid-based time-temperature indicator for monitoring food product quality. J. Food. Eng., 100: 427-434.
8. Rukchon, C., Nopwinyuwong, A., Trevanich, S., Jinkarn, T. and Suppakul, P. (2014) Development of food spoilage indicator for monitoring freshness of skinless chicken breast. Talanta, 130: 547-554.
9. Xiao-Wei, H., Xia-Bo, Z., Ji-Yong, S., Yanin, G., Jie-Wen, Z., Jianchun, Z. and Limin, H. (2014) Determination of pork spoilage by colorimetric gas sensor array based on natural pigments. Food Chem., 145: 549-555.
10. Rukchon, C., Trevanich, S., Jinkarn, T. and Suppaku P. (2011) Volatile compounds as quality indicators of fresh chicken & possible application in intelligent packaging. In: 12th Asian Food Science Conference, Bangkok, Thailand.
11. Kuswandi, B., Jayus, Larasati, T.S., Abdullah, A. and Heng, L.Y. (2012) Real time monitoring of Shrimp spoilage using on package sticker sensor based on natural dye of curcumin. Food Anal. Methods, 5: 882-888.
12. Smolander, M., Hurme, E., Latva-Kala, K., Luoma, T., Alakomi, H.L. and Ahvenainen, R. (2002) Myoglobin based indicators for the evaluation of freshness of unmarinated broiler cuts. Innov. Food Sci. Emerg. Technol., 3: 277-85.
13. Wallach, F.H. and Hollis, N.H. (2012) Methods and devices for detecting microbial spoilage in food products. US patent No. 6495368.
14. Khalil, G.E., Putnam, D.L. and Hubbard, T.W. (2010) Ammonia detection and measurement device, US Patent Application No. 2010/0330, 692.
15. Pearson, D. (1968) Methods related to protein breakdown. J. Sci. Food Agric., 19: 366-369.
16. Strange, E.D., Benedict, R.C., Smith, J.L. and Swift, C.E. (1977) Evaluation of rapid test for monitoring alteration in meat quality during storage. J. Food Protect., 40: 843-847.
17. Washko, M.E. and Rice, E.W. (1961) Determination of glucose by an improved enzymatic procedure. Clin. Chem., 7, 542-545.
18. APHA. (2001) Compendium of methods for the microbiological examination of foods. 4th ed. Washington DC: American Public Health Association.
19. ICMSF (1980) Microorganisms in Foods. 2nd ed. International Commission on Microbiological Specification for Food, New Jersey, United States.
20. Byun, J.S., Min, J.S., Kim, I.S., Kim, J.W., Chung, M.S. and Lee, M. (2003) Comparison of indicator of microbial quality of meat during aerobic cold storage. J. Food Protect., 66, 1733-1737.
21. Rokka, M., Eerola, S., Smolander, M., Alakomi, H.L. and Ahvenainen, R. (2004) Monitoring of the quality of modified atmosphere packaged broiler chicken curs stored in different temperature conditions. Biogenic amines as quality-indicating metabolites. Food Control, 15: 601-607.
22. Nychas, G.J.E, Skandamis, P.N., Tassou, C.C. and Koutsoumanis, K.P. (2008) Meat spoilage during distribution. Meat Sci., 78: 77-89.