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              Research 
              
(Published online: 28-03-2015) 
              
              27.
              
              Study on hematological alterations induced 
              by amphistomosis in buffaloes - Vandip. D. Chauhan, P. 
              V. Patel, Jigar J. Hasnani, Suchit S. Pandya, Sunanda Pandey, 
              Dhaval V. Pansuriya and Vijayata Choudhary 
              
              Veterinary World, 8(3): 417-420   
              
   
                
                
doi: 
              10.14202/vetworld.2015.417-420   Vandip. 
              D. Chauhan: 
              Department of Veterinary Parasitology, College of Veterinary 
              Science and Animal Husbandry, Anand Agricultural University, Anand, 
              Gujarat, India; drvandip@gmail.com P. V. 
              Patel: 
              Department of Veterinary Parasitology, College of Veterinary 
              Science and Animal Husbandry, Anand Agricultural University, Anand, 
              Gujarat, India; pvpatel2110@gmail.com Jigar 
              J. Hasnani: 
              Department of Veterinary Parasitology, College of Veterinary 
              Science and Animal Husbandry, Anand Agricultural University, Anand, 
              Gujarat, India; jhasnani@gmail.com Suchit 
              S. Pandya: 
              Department of Veterinary Parasitology, College of Veterinary 
              Science and Animal Husbandry, Anand Agricultural University, Anand, 
              Gujarat, India; pandyasuchit@gmail.com Sunanda 
              Pandey: 
              Department of Veterinary Pathology, College of Veterinary Science 
              and Animal Husbandry, Anand Agricultural University, Anand, 
              Gujarat, India; drsunandapandey@gmail.com Dhaval 
              V. Pansuriya: 
              Department of Veterinary Surgery and Radiology, College of 
              Veterinary Science and Animal Husbandry, Navsari Agricultural 
              University, Navsari, Gujarat, India; dhaval.pansuria@gmail.com 
              Vijayata Choudhary: 
              
              Department of Veterinary Parasitology, College of Veterinary 
              Science and Animal Husbandry, Anand Agricultural University, Anand, 
              Gujarat, India; dr.viz.vet@gmail.com   
              Received: 26-12-2014, Revised: 15-02-2014, Accepted: 23-02-2015, 
              Published online: 28-03-2015   
              
              
              Corresponding author: 
              
              V. D. Chauhan, e-mail: drvandip@gmail.com 
 
              Citation:Chauhan VD, Patel PV, 
              Hasnani JJ, Pandya SS, Pandey S, Pansuriya DV, Choudhary V (2015). 
              Study on hematological alterations induced by amphistomosis in 
              buffaloes, Veterinary World 8(3):417-420. 
 
              Abstract 
 Aim:
              The study was undertaken to compare the alterations in the 
              hematological parameters in buffaloes suffering from Amphistomosis 
              with normal buffaloes and to correlate it with the subclinical 
              infection that is hard to diagnose. 
              Materials and Methods: Blood samples from 50 amphistomes 
              infected as well as 50 non-infected buffaloes from slaughter 
              houses were taken into vacutainer tubes containing ethylene 
              diamine tetraacetic acid for estimation of various hematological 
              parameters by Automatic Analyzer Hema-2062 manufactured by 
              Analytical Technologies Ltd.  
              Result: There was a significant reduction in 
              the mean hemoglobin, total leukocyte count, total erythrocyte 
              count and packed cell volume and significant increase in the 
              neutrophils count and eosinophil count of infected buffaloes as 
              compared to the non-infected buffaloes respectively. 
              Conclusion: Amphistomosis is characterized by severe 
              neutrophilia, eosinophilia, and anemia. Anemia of high intensity 
              along with hepatic damage can lead to the death of the animal in 
              severe cases. Alterations in the Hematological parameters can be 
              used as an indicator to diagnose and check the severity of 
              amphistomosis especially in young ones and in subclinical 
              infection. 
              Keywords: amphistomosis, hematology, 
              eosinophilia, anemia, neutrophilia. 
 
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