Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916

ISSN (Print): 0972-8988


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License

( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online: 28-03-2015)

28. Comparative antibiogram of coagulase-negative Staphylococci (CNS) associated with subclinical and clinical mastitis in dairy cows - B. K. Bansal, D. K. Gupta, T. A. Shafi and S. Sharma

Veterinary World, 8(3): 421-426



   doi: 10.14202/vetworld.2015.421-426


B. K. Bansal: Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 1410 04, Punjab, India;

D. K. Gupta: Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 1410 04, Punjab, India;

T. A. Shafi: Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 1410 04, Punjab, India;

S. Sharma: Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 1410 04, Punjab, India;


Received: 01-11-2014, Revised: 19-02-2015, Accepted: 01-03-2015, Published online: 28-03-2015


Corresponding author: D. K. Gupta, e-mail:

Citation: Bansal BK, Gupta DK, Shafi TA, Sharma S (2015) Comparative antibiogram of coagulase-negative Staphylococci associated with subclinical and clinical mastitis in dairy cows, Veterinary World 8(3): 421-426.

Aim: The present study was planned to determine the in vitro antibiotic susceptibility of coagulase-negative Staphylococci (CNS) strains isolated from clinical and subclinical cases of mastitis in dairy cows. Antibiotic sensitivity profile will be helpful to recommend early therapy at the field level prior to availability of CST results.

Materials and Methods: The milk samples from cases of clinical mastitis received in Mastitis Laboratory, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana and those of subclinical mastitis collected during routine screening of state dairy farms, were subjected to microbial culture. Identification of CNS organisms was done by standard biochemical tests. Antibiotic sensitivity testing, based on 30 antibiotics belonging to 12 groups, was done on 58 randomly selected CNS isolates (clinical isolates: 41, subclinical isolates: 17).

Results: Isolates were highly susceptible to chloramphenicol (98.3%), gentamicin (93.1%), streptomycin (91.4%), linezolid (91.4%), ceftixozime (87.9%), cloxacillin (86.2%), clotrimazole (86.2%), bacitracin (86.2%), enrofloxacin (84.5%) and ceftrioxone + tazobactum (70.7%), while resistance was observed against amoxicillin (77.6%), penicillin (75.9%), ampicillin (74.1%) and cefoperazone (51.7%). Overall, isolates from clinical cases of mastitis had a higher resistance than subclinical isolates.

Conclusion: CNS isolates were susceptible to chloramphenicol, gentamicin and streptomycin, while higher resistance was recorded against routinely used penicillin group.

Keywords: antibiotic, coagulase-negative staphylococci, cows, culture and mastitis.

1. Thorberg, B.M., Dnielsson-Tham, E.L., Emanuelson, U. and Waller, P. (2009) Bovine subclinical mastitis caused by different types of coagulase-negative staphylococci. J. Diary. Sci., 92: 4962-4970.
2. Denis, M., Lacy-Hulbert, S.J., Buddle, B.M., Williamson, J.H. and Wedlock, D.N. (2011) Streptococcus uberis-specific T cells are present in mammary gland secretions of cows and can be activated to kill S. uberis. Vet. Res. Community, 35: 145-156.
3. Bansal, B.K. and Gupta, D.K. (2009) Economic analysis of bovine mastitis in India and Punjab- A review. Indian J. Dairy Sci., 62(5): 337-45.
4. Honkanen-Buzalski, T., Myllys, V. and Pyorala, S. (1994) Bovine clinical 338 mastitis due to coagulase-negative staphylococci and their susceptibility to antimicrobials. J. Vet. Med., 41: 344.
5. Taponen, S., Supré, K., Piessens, V., Van Coillie, E., De Vliegher, S. and Koort, J. (2012) Staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis. Int. J. Syst. Evol. Microbiol., 62: 61-65.
6. Supre, K., Haesebrouck, F., Zadoks, R.N., Vaneechoutte, M., Piepers, S. and De-Vliegher S. (2011) Some coagulase-negative Staphylococcus species affect udder health more than others. J. Dairy Sci., 94: 2329-2340.
7. Pyorala, S. and Taponen, S. (2009) Coagulase-negative staphylococci emerging mastitis pathogens. Vet. Microbiol., 134: 3-8.
8. Taponen, S., Koort, J., Bjorkroth, J., Saloniemi, H. and Pyorala, S. (2007) Bovine intra-mammary infections caused by coagulase-negative staphylococci may persist throughout lactation according to amplified fragment length polymorphism-based analysis. J. Dairy Sci., 90: 3301-3307.
9. Waller, K.P., Aspa, A., Nyman, A., Persson, Y. and Andersson, U.G. (2011) CNS species and antimicrobial resistance in clinical and subclinical bovine mastitis. Vet. Microbiol., 152: 112-116.
10. National Mastitis Council Inc. (1990) Microbiological Procedures for the Diagnosis of Bovine Udder Infections. National Mastitis Council Inc., Arlington, VA, USA.
11. Quinn, P.J., Carter, M.E., Markey, B.K. and Carter, G.R. (2000) Clinical Veterinary Microbiology. Mosby-Year Book Europe Limited, London.
12. Pitkala, A., Haveri, M., Pyorala, S., Myllys, V. and Honkanen-Buzalski, T. (2004) Bovine mastitis in Finland 2001- Prevalence, distribution of bacteria, and antimicrobial resistance. J Dairy Sci., 87(8): 2433-2441.
13. Tenhagen, B.A., Koster, G., Wallman, J. and Heuwieser, W. (2006) Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. J. Dairy Sci., 89: 2542-2551.
14. Ferguson, J.D., Azzaro, G., Gambina, M. and Licitra, G. (2007) Prevalence of mastitis pathogens in Ragusa, Sicily, from 2000 to 2006. J. Dairy Sci., 90: 5798-5813.
15. Nam, H.M., Kim, J.M., Lim, S.K., Jang, K.C. and Jung, S.C. (2010) Infectious aetiologies of mastitis on Korean dairy farms during 2008. Res. Vet. Sci., 88: 372-374.
16. Piessens, V., Van-Coillie, E., Verbist, B., Supré, K., Braem, G., Van-Nuffel, A., De-Vuyst, L., Heyndrickx, M. and De-Vliegher, S. (2011) Distribution of coagulase-negative Staphylococcus species from milk and environment of dairy cows differs between herds. J. Dairy Sci., 94: 2933-2944.
17. Abrahmsen, M., Persson, Y., Kanyima, M.B. and Bage, R. (2012) Prevalence of subclinical mastitis in dairy farms in urban and peri-urban areas of Kampala, Uganda. Trop. Anim. Health Prod., 46(1): 99-105.
PMid:23955012 PMCid:PMC3895220
18. Byarugaba, D.K., Nakavuma, J.L., Vaarst, M. and Laker, C. (2008) Mastitis occurrence and constraints to mastitis control in smallholder dairy farming systems in Uganda. Livest. Res. Rural Dev., 20: 1-3.
19. Haftu, R., Habtamu, T., Getachew, G. and Kalayou, S. (2012) Prevalence, bacterial causes, and antimicrobial susceptibility profile of mastitis isolates from cows in large-scale dairy farms of Northern Ethiopia. Trop. Anim. Health Prod., 44: 1765-1771.
20. Karimuribo, E.D., Fitzpatrick, J.L., Bell, C.E., Swai, E.S., Kambarage, D.M., Ogden, N. H., Bryant, M.J. and French, N.P. (2006) Clinical and subclinical mastitis in smallholder dairy farms in Tanzania: Risk, intervention and knowledge transfer. Prev. Vet. Med., 74: 83-98.
21. Gianneechini, R., Concha, C., Rivero, R., Delucci, I. and Moreno Lopez, J. (2002) Occurrence of clinical and sub-clinical mastitis in dairy herds in the West Littoral region in Uruguay. Acta Vet. Scand., 43: 221-230.
PMid:12831175 PMCid:PMC1764198
22. Gentilini, E., Denamiel, G., Betancor, A., Rebuelto, M., Rodriguez, M. and De-Torrest, R.A. (2002) Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis in Argentina. J. Dairy Sci., 85: 1913-1917.
23. Rajala-Schultz, P.J., Torres, A.H., Degraves, F.J., Gebreyes, W.A. and Patchanee, P. (2009) Antimicrobial resistance and genotypic characterization of coagulase-negative staphylococci over the dry period. Vet. Microbiol., 134: 55-64.
24. Kenar, B., Kuyucuoğlu, Y. and Şeker, E. (2012) Antibiotic susceptibility of coagulase-negative staphylococci isolated from bovine subclinical mastitis in Turkey. Pak. Vet. J., 32(3): 390-393.
25. Odd, G.B. and Maeland, J.A. (1997) Mechanisms of methicillin resistance instaphylococci. AMPIS, 105: 264-276.
26. Archer, G.L. and Scott, J. (1991) Conjugative transfer genes in staphylococcal isolates from the United States. Antimicrob. Agents Chemother., 35: 2500-2504.
PMid:1810183 PMCid:PMC245420
27. Myllys, V., Asplund, K., Brofeldt, E., Hirvela-Koski, V., Honkanen-Buzalski, T., Junttila, J., Kulkas, L., Myllykangas, O., Niskanen, M., Saloniemi, H., Sandholm, M. and Saranpaa, T. (1998) Bovine mastitis in Finland in 1988 and 1995–changes in prevalence and antimicrobial resistance. Acta. Vet. Scand., 39: 119-126.
28. Dhakal, I.P., Dhakal, P., Koshihara, T. and Nagahata, H. (2007) Epidemology and Bacteriology survey of buffalo mastitis in Nepal. J Vet. Med. Sci., 69: 1241-1245.
29. Sumathia, B.R., Veeregowda, B.M. and Gomes, A.R. (2008) Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Vet. World, 1(8): 237-238.
30. Anakalo, S., Gathoni, A., Tura, G. and Milcah, M. (2004) Prevelence of bovine mastitis amongst small holder dairy herds in Kenya. Isr. J. Vet. Med., 59: 1-2.
31. Alekish, M.O., Al-Qudah, K.M. and Al-Saleh, A. (2013) Prevalence of antimicrobial resistance among bacterial pathogens isolated from bovine mastitis in northern Jordan. Rev. Méd. Vét., 164(6): 319-326.
32. Luthje, P. and Schwarz, S. (2006) Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide-lincosamide resistance phenotypes and genotypes. J. Antimicrob. Chemother, 57: 966-969.
33. Mahami, T., Odonkor, S., Yaro, M. and Adu-Gyamfi, A. (2011) Prevalence of antibiotic resistant bacteria in milk sold in Accra. Int. Res. J. Microbiol., 2(4): 126-132.
34. Kalmus, P., Aasmae, B., Karssin, A., Orro, T. and Kask, K. (2011) Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia. Acta Vet. Scand., 53(1): 4.
PMid:21299911 PMCid:PMC3041692