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R esearch
(Published online:
04-05-2015)
1.
Seroprevalence and comparison of different
serological tests for brucellosis detection in small ruminants
- Dashrath B. Sadhu, H. H. Panchasara, H. C. Chauhan, D. R.
Sutariya, V. L. Parmar and H. B. Prajapati
Veterinary World, 8(5): 561-566
doi:
10.14202/vetworld.2015.561-566
Dashrath B. Sadhu:
Department of Veterinary Medicine, Livestock Research Station,
Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar, Dantiwada, Gujarat, India; dr.dashrathsadhu@gmail.com
H. H.
Panchasara:
Department of Veterinary Medicine, Livestock Research Station,
Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar, Dantiwada, Gujarat, India; harshadlrs@yahoo.co.in
H. C.
Chauhan:
Department of Animal Biotechnology, College of Veterinary Science
& Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural
University, Sardarkrushinagar, Dantiwada, Gujarat, India;
dr.chauhan123@gmail.com
D. R.
Sutariya:
Department of Veterinary Medicine, Livestock Research Station,
Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar, Dantiwada, Gujarat, India; dr.dren087@gmail.com
V. L.
Parmar:
Department of Veterinary Medicine, Livestock Research Station,
Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar, Dantiwada, Gujarat, India; viki07290@gmail.com
H. B.
Prajapati: Department of Veterinary Medicine, Livestock
Research Station, Sardarkrushinagar Dantiwada Agricultural
University,
Sardarkrushinagar, Dantiwada, Gujarat, India;
hit.vet305@gmail.com
Received:
16-12-2014, Revised: 22-03-2015, Accepted: 30-03-2015, Published
online: 04-05-2015
Corresponding author:
Dashrath B. Sadhu, e-mail: dr.dashrathsadhu@gmail.com
Citation: Sadhu DB,
Panchasara HH, Chauhan HC, Sutariya DR, Parmar VL, Prajapati HB
(2015) Seroprevalence and comparison of different serological
tests for brucellosis detection in small ruminants, Veterinary
World 8(5):561-566.
Abstract
Aim:
The aim was to study the
seroprevalence and efficacy of the different serological tests
used for detection of antibody against
Brucella species in small
ruminants of Banaskantha district of North-Gujarat.
Materials and Methods: Total
1000 serum samples comprising of 485 from sheep and 515 from goat
tested for detection of antibodies against the
Brucella
species by three different serological tests
viz., Rose
bengal plate test (RBPT), Standard tube agglutination test (STAT),
and Indirect Enzyme-linked immunosorbent assay (I-ELISA).
Results: The seroprevalence of
brucellosis in small ruminants was 11.30%, 11.10%, and 8.80% by
RBPT, STAT, and I-ELISA, respectively. The seroprevalence of
brucellosis was found to be higher in sheep than goats. The
sensitivity of RBPT was found slight more than STAT, but the
specificity of both tests was same. In this study, the overall
agreement of RBPT and STAT with I-ELISA was found 92.50% and
92.30% in small ruminants, respectively.
Conclusion: I-ELISA was a
better serological test as compared to RBPT and STAT in the sense
of sensitivity, specificity, and rapidity and it could be
advocated for screening of brucellosis in sheep and goats.
Keywords:
brucellosis, seroprevalence, serological test, small ruminant.
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