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R esearch
(Published online:
15-05-2015)
10.
Genetic polymorphism of toll-like receptors
4 gene by polymerase chain reaction-restriction fragment length
polymorphisms, polymerase chain reaction-single-strand
conformational polymorphism to correlate with mastitic cows
- Pooja H. Gupta, Nirmal A. Patel, D. N. Rank and C. G. Joshi
Veterinary World, 8(5): 615-620
doi:
10.14202/vetworld.2015.615-620
Pooja
H. Gupta:
Department of Biochemistry, B. A. College of Agriculture, Anand
Agriculture University, Anand, Gujarat, India;
guptapooja333@gmail.com
Nirmal
A. Patel:
Department of Animal Genetics and Breeding, College of Veterinary
Science and Animal Husbandry, Anand, Gujarat,
India;
nirmal823@gmail.com
D. N.
Rank:
Department of Animal Genetics and Breeding, College of Veterinary
Science and Animal Husbandry, Anand, Gujarat,
India;
dnrank@gmail.com
C. G.
Joshi: Department of Animal Biotechnology, College of
Veterinary Science and Animal Husbandry, Anand, Gujarat,
India;
cgjoshi@aau.in
Received:
20-12-2014, Revised: 05-04-2015, Accepted: 15-04-2015, Published
online: 15-05-2015
Corresponding author:
Pooja H. Gupta, e-mail: guptapooja333@gmail.com
Citation:
Gupta PH, Patel NA,
Rank DN, Joshi CG (2015) Genetic polymorphism of toll-like
receptors 4 gene by polymerase chain reaction-restriction
fragment length polymorphisms, polymerase chain reaction-single-strand
conformational polymorphism to correlate with mastitic cows,
Veterinary World 8(5):615-620.
Abstract
Aim:
An attempt has been made to
study the toll-like receptors 4 (TLR4) gene polymorphism from
cattle DNA to correlate with mastitis cows.
Materials and Methods: In
present investigation, two fragments of TLR4 gene named T4CRBR1
and T4CRBR2 of a 316 bp and 382 bp were amplified by polymerase
chain reaction (PCR), respectively from Kankrej (22) and Triple
cross (24) cattle. The genetic polymorphisms in the two
populations were detected by a single-strand conformational
polymorphism in the first locus and by digesting the fragments
with restriction endonuclease Alu
I in the second one.
Results: Results showed that
both alleles (A and B) of two loci were found in all the two
populations and the value of polymorphism information content
indicated that these were highly polymorphic. Statistical results
of χ2
test indicated that two polymorphism
sites in the two populations fit with Hardy–Weinberg equilibrium
(p˂0.05). Meanwhile, the effect of polymorphism of TLR4 gene on
the somatic cell score (SCS) indicated the cattle with allele a in
T4CRBR1 showed lower SCS than that of allele B (p<0.05). Thus, the
allele A might play an important role in mastitis resistance in
cows.
Conclusion: The relationship
between the bovine mastitis trait and the polymorphism of TLR4
gene indicated that the bovine TLR4 gene may play an important
role in mastitis resistance.
Keywords:
cattle, mastitis, restriction fragment length polymorphisms,
single-strand conformational polymorphism, somatic cell score,
toll-like receptors 4 gene.
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